Issues

Summary: Deep-learning methods are introduced for users, highlighting accessible methods for 3D nucleus analysis and encouraging interdisciplinary research between computer scientists and biologists.

Summary: Dopey proteins regulate membrane trafficking and play crucial roles from morphogenesis to neural function, but remain poorly understood. This Review discusses these fascinating enigmas.

Summary: Multi-nucleate Dictyostelium cells divide by unilateral cleavage furrows that progress and expand according to a pattern of cortexillin and myosin II that is determined by microtubule asters at the spindle poles.

Summary: VSMCs are stimulated by platelet microvesicles via PRKAA after vascular injury, and Compound C treatment is a potential therapy for neointimal hyperplasia.

Summary: Targeted RNAi or overexpression of Peroxin genes in Drosophila fat body or cells revealed peroxisome independent roles in lipid storage, with Pex14 causing the strongest phenotypes.

Summary: Phosphorylated paxillin and phosphorylated FAK form patterns in focal adhesions, creating local signaling hubs in a regulated manner.

Summary: The secreted protein kinase VLK is released from hepatocytes and protects the liver from perivascular fibrosis and inflammation.

Summary: DOCK family GEFs are master regulators of cellular organization. We discovered that tandem C2 domains in the superfamily members enable GEF clustering at specialized sites of full activation.

Summary: Histone variant macroH2A1.1 binding to the TSS of genes dependent on Pol II pausing stimulates transcription by promoting Pol II release in a human triple-negative breast cancer cell model.

Highlighted Article: Cryo-electron tomographic reconstruction of neuronal growth cone subdomains reveals distinctive F-actin and microtubule cytoskeleton architectures and modulation at molecular detail.

Summary: Deregulation of vesicular trafficking by upregulated flotillins involves sphingosine kinase 2, and contributes to high AXL levels and to epithelial-to-mesenchymal transition in breast cancer cells.

Highlighted Article: The oligopeptide transporter Slc15A is a novel macropinocytosis regulator in Dictyostelium and part of a positive feedback mechanism, coupling nutrient status and macropinocytosis.

Highlighted Article: Thrombin and protease-activated receptor agonists induce dynamic blebbing in breast cancer cells, which can be attenuated by contact-mediated compression, and activation of the mechanosensitive ion channel Piezo1.

Summary: Analyses of mouse embryos and cardiomyocytes differentiated from human embryonic stem cells show that SIRT1 regulates cardiomyocyte alignment during development, in part through chemotaxis.