CELL SCIENCE AT A GLANCE
Summary: The features of chromosome folding and organization are important for proper nuclear function. This Cell Science at a Glance article reviews chromosome and genome organization dynamics through the cell cycle.
A cytoplasmic protein kinase couples engagement of Chlamydomonas ciliary receptors to cAMP-dependent cellular responses
Highlighted Article: A protein kinase in the cytoplasm is phosphorylated within 1 min after engagement of ciliary receptors during Chlamydomonas fertilization and is required to trigger the fusion-essential increase in cytoplasmic cAMP.
Analysis of monocyte cell tractions in 2.5D reveals mesoscale mechanics of podosomes during substrate-indenting cell protrusion
Summary: Using a buckling model, we extend the current description of local podosome protrusion and include a mechanical explanation for protrusion on the cell scale.
Summary: This study reports that suspended tumor cells respond to fluid shear stress through histone acetylation-mediated nuclear expansion, which protects CTCs from shear-induced apoptosis.
Summary: A chemical probe-based activity assay was used to show that inhibition of tripeptidyl peptidase II (TPPII) by serpinB2 regulates proteostasis in senescent cells.
Interactions of cytosolic tails in the Jen1 carboxylate transporter are critical for trafficking and transport activity
Summary: Interaction of cytosolic terminal regions in the Jen1 carboxylate transporter, belonging to the major facilitator superfamily, are critical for trafficking and transport activity.
Summary: We show that yeast ESCRT-III interacts with Vps68, an endosomal membrane protein. We propose that Vps68 and ESCRT-III cooperate in the abscission of intraluminal vesicles by weakening the lipid bilayer.
Highlighted Article: Characterization of new roles for retromer in C. elegans development and in the regulation of ciliogenesis in mammalian cells, suggesting a novel role for retromer in CP110 removal from the mother centriole.
TOOLS AND RESOURCES
Single-cell analysis reveals the Comma-1D cell line as a unique model for mammary gland development and breast cancer
Summary: Characterization at a single-cell level of the unique heterogeneity of the mammary epithelial Comma-1D cell line, which illustrates its use as an experimental model to study both differentiation and transformation.
Summary: General Image Analysis of Nuclei-based Images (GIANI) is a new plugin for the popular FIJI platform, designed for the automated analysis of 3D microscopy images of a wide range of sample types.