Issues

Summary: Histone variants change the structural properties of nucleosomes by wrapping more or less DNA, altering nucleosome stability or carrying out specialized functions.

Summary: This Review dissects what microscope settings should be included in a publication and the reasons they are essential for scientific reproducibility and data verification.

Summary: This Review dissects what image processing parameters should be included in a publication, and the reasons they are essential for scientific reproducibility and data verification.

Summary: This Review focuses on forces generated by pericytes, solid and fluid mechanical forces exerted on pericytes, and the mechanobiological responses of pericytes to these forces.

Summary: p65 localization to the nucleus acts as a molecular switch to reverse therapy-induced senescence in GBM cells; therefore, use of p65 inhibitors in combination with senotherapeutics can induce permanent senescence of GBM cells.

Highlighted Article: We propose a mechanism by which vimentin tunes collagen-dependent cell migration by modulating β1 integrin activation and clustering through a paxillin–talin molecular complex at focal adhesions.

Summary: The constitutively phosphorylated H2A-S129E mutant shows enhanced chromatin dynamics due to structural modifications, leading to efficient checkpoint and NHEJ repair, even in the absence of Rad9.

Summary: The COPII coat protein SEC24A appears to have a moonlighting function in mediating contacts between mitochondria and ER.

Summary: A microtubule polymerising factor, Orbit, localises on centrioles and determines centriole length by antagonising Klp10A in Drosophila spermatocytes.

Summary: The voltage-gated sodium channel β2 subunit associates with cholesterol-rich lipid rafts by means of S-palmitoylation, which may contribute to establishing its polarized cell surface localization.

Summary: A new form of nuclear movement in fission yeast, termed microtubule-independent nuclear movement, that is dependent on actin cables and on proteins involved in ER–plasma membrane contacts is described.

Summary: Transgenic and expression analyses reveal a complex set of molecular features that function together as address labels to target opsin to the outer segment.

Highlighted Article: Depletion of Hoka, a novel septate junction protein, from the Drosophila midgut results in the disruption of septate junctions, intestinal barrier dysfunction, stem cell overproliferation and epithelial tumors.

Summary: A nuclear export sequence in Nup214 may help to localize the protein to the cytoplasmic side of the nuclear pore complex.

Highlighted Article: The mRNA-binding protein THOC4 modulates mRNA stability of TFEB for the maintenance of energy homeostasis during prolonged starvation.

Summary: DROSHA is part of the primary response to DNA damage and is recruited at double-strand breaks in an MRN-dependent manner. DROSHA fosters NHEJ repair and inhibits HR by controlling 53BP1 recruitment.

Highlighted Article: The lamin A/C H222P laminopathy mutation provides insights into lamin substructure organization.

Highlighted Article: Unexpected retention of p120 (and E-cadherin) in lung metastases from ductal breast cancer highlights prominent roles in late stage events associated with seeding and/or colonization of distant metastatic sites.

Summary: Previous reports of axon initial segment Ranbp2 are shown to be based on NF186-cross-reacting antibodies and the intrinsic ability of an N-terminal fragment of Ranbp2 to bind to stable microtubules.

Summary: Comparative transcriptomics and metabolomics analyses of two anatomically different regions of human bone marrow revealed them to be functionally distinct hematopoietic stem cell niches.

Summary: Clarification of the relationship between two tension-regulated proteins, LIMD1 and TRIP6, and identification of a role for TRIP6 in regulating the organization of the actin cytoskeleton.

Summary: Methods of releasing calcium from lysosomes are limited but characterization of the effects of GPN in primary cultured human fibroblasts confirmed that it probably targets acidic organelles.