Issues
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Cover image
Cover Image
Cover: The image depicts a mouse oocyte at around metaphase-I of the meiotic cell cycle. The oocyte was fixed and stained for actin filaments (magenta), phosphorylated ezrin/radixin/moesin (green), and chromosomes (orange). Before release from prophase arrest, the oocyte was injected with a cRNA encoding LIMK1, in order to force cofilin phosphorylation and inactivation. The culture medium was also supplemented with 100 µM CK-666 in order to inhibit the Arp2/3 complex. These treatments promoted extensive elongation of membrane microvilli and their cytoplasmic actin rootlets, while the cytoplasmic F-actin mesh was depleted. See article by A. Bourdais et al. (jcs259237).
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RESEARCH HIGHLIGHTS
FIRST PERSON
MEETING REPORT
REVIEW
The role of mitochondrial dynamics in mtDNA maintenance
Summary: A review of how the dynamic nature of the mitochondrial network is important for maintenance of the mitochondrial genome.
RESEARCH ARTICLES
The laminin-binding integrins regulate nuclear factor κB-dependent epithelial cell polarity and inflammation
Highlighted Article: Laminin-binding integrins play a role in maintaining epithelial cell polarity and decrease pro-inflammatory cytokine secretion by regulating NF-κB-dependent signaling.
Force-induced changes of α-catenin conformation stabilize vascular junctions independently of vinculin
Summary: There are novel antibody epitopes at the actin-binding domain of α-catenin that correlate with high affinity binding and are exposed in junction-stabilizing VE-cadherin–α-catenin fusion proteins.
Structural model of human PORCN illuminates disease-associated variants and drug-binding sites
Summary: We developed and validated a computational model of PORCN that elucidates the catalytic mechanism, disease-associated mutations and function of inhibitors of this MBOAT protein.
Cofilin regulates actin network homeostasis and microvilli length in mouse oocytes
Highlighted Article: Preventing cofilin activation at meiosis resumption in oocytes leads to microvillar actin overgrowth and depletion of cytoplasmic F-actin.
The tumor-suppressive long noncoding RNA DRAIC inhibits protein translation and induces autophagy by activating AMPK
Summary: The tumor-suppressive lncRNA DRAIC represses protein translation and stimulates autophagy through the activation of AMP-activated protein kinase (AMPK).
A single-molecule localization microscopy method for tissues reveals nonrandom nuclear pore distribution in Drosophila
Summary: Description of a simple line scanning microscope for super-resolution imaging of tissues using DNA-PAINT. This reveals a non-random distribution of nuclear pores in most Drosophila cell-types.
IP6K1 upregulates the formation of processing bodies by influencing protein-protein interactions on the mRNA cap
Summary: IP6K1, independent of its enzyme activity, engages in protein-protein interactions on the mRNA cap to promote translational suppression and upregulate the formation of processing bodies.
Cell cycle progression and transmitotic apoptosis resistance promote escape from extrinsic apoptosis
Summary: Cancer cells can escape extrinsic apoptosis by approaching mitosis, where transiently high Mcl-1 expression prevents caspase-triggered apoptosis execution.
Crosstalk between the calcineurin and cell wall integrity pathways prevents chitin overexpression in Candida albicans
Summary: The pathogenic fungus Candida can limit the overproduction of chitin in its cell wall to prevent loss of fitness via a calcineurin-dependent mechanism.
WTS-1/LATS regulates endocytic recycling by restraining F-actin assembly in a synergistic manner
Summary: WTS-1, the C. elegans LATS homolog, synergistically maintains endosomal actin homeostasis by restraining PTRN-1-mediated F-actin assembly and facilitating UNC-60A-mediated F-actin disassembly, thereby sustaining endocytic recycling.
DUSP6 regulates radiosensitivity in glioblastoma by modulating the recruitment of phosphorylated DNAPKcs at DNA double-strand breaks
Summary: DUSP6 is upregulated in recurrent glioblastoma and has cytoplasmic and nuclear localization. DUSP6 inhibition radiosensitizes glioblastoma cells via impaired recruitment of p-DNAPKcs, increasing progression-free survival in vivo.
ALG2 regulates type I interferon responses by inhibiting STING trafficking
Highlighted Article: ALG2 is a regulating factor for STING trafficking from endoplasmic reticulum to perinuclear compartments, and Ca2+-binding ability is crucial for the function of ALG2 in this process.
Frizzled3 inhibits Vangl2–Prickle3 association to establish planar cell polarity in the vertebrate neural plate
Summary: Frizzled3 induces Vangl2 phosphorylation and promotes PCP in Xenopus neuroectoderm by inhibiting the formation of the anterior Vangl2–Prickle3 complex.
TOOLS AND RESOURCES
Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system
Highlighted Article: We established conditional knockout cell lines for Rab1 and Rab5, and found that loss of Rab1 induced perinuclear accumulation of early endosomes and that Rab5 was dispensable for lysosome biogenesis.
Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system
Summary: A genetically encoded phycocyanobilin biosynthesis system and plasmid series for use of phycocyanobilin as an iRFP chromophore enables bright near-infrared fluorescence imaging in fission yeast.
Imaging Cell Architecture and Dynamics
We are still welcoming submissions for our upcoming Special Issue: Imaging Cell Architecture and Dynamics. This issue will be coordinated by two Guest Editors: Lucy Collinson (The Francis Crick Institute, UK) and Guillaume Jacquemet (University of Turku, Finland). Extended submission deadline: 29 March 2024.
Journal of Cell Science - more than just a journal
People who know JCS well will know that we're more than just a journal and that our community – the cell biology community – really is at the heart of everything we do. Read the full Editorial by Editor-in-Chief Michael Way and Executive Editor Seema Grewal.
2024 Journal Meeting 'Diversity and Evolution in Cell Biology'
Registration is open for our 2024 Journal Meeting Diversity and Evolution in Cell Biology, which aims to bring together evolutionary biologists and cell biologists investigating diverse aspects of cellular physiology. Submit your abstract by 5 April. Final registration deadline: 3 May 2024.
Workshop: Roles of Lipids in Nuclear Homeostasis and Genome Stability
Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place 14-17 October 2024. Application deadline: 19 April.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.