Summary: A review of how the dynamic nature of the mitochondrial network is important for maintenance of the mitochondrial genome.
The laminin-binding integrins regulate nuclear factor κB-dependent epithelial cell polarity and inflammation
Highlighted Article: Laminin-binding integrins play a role in maintaining epithelial cell polarity and decrease pro-inflammatory cytokine secretion by regulating NF-κB-dependent signaling.
Force-induced changes of α-catenin conformation stabilize vascular junctions independently of vinculin
Summary: There are novel antibody epitopes at the actin-binding domain of α-catenin that correlate with high affinity binding and are exposed in junction-stabilizing VE-cadherin–α-catenin fusion proteins.
Summary: We developed and validated a computational model of PORCN that elucidates the catalytic mechanism, disease-associated mutations and function of inhibitors of this MBOAT protein.
Highlighted Article: Preventing cofilin activation at meiosis resumption in oocytes leads to microvillar actin overgrowth and depletion of cytoplasmic F-actin.
The tumor-suppressive long noncoding RNA DRAIC inhibits protein translation and induces autophagy by activating AMPK
Summary: The tumor-suppressive lncRNA DRAIC represses protein translation and stimulates autophagy through the activation of AMP-activated protein kinase (AMPK).
A single-molecule localization microscopy method for tissues reveals nonrandom nuclear pore distribution in Drosophila
Summary: Description of a simple line scanning microscope for super-resolution imaging of tissues using DNA-PAINT. This reveals a non-random distribution of nuclear pores in most Drosophila cell-types.
IP6K1 upregulates the formation of processing bodies by influencing protein-protein interactions on the mRNA cap
Summary: IP6K1, independent of its enzyme activity, engages in protein-protein interactions on the mRNA cap to promote translational suppression and upregulate the formation of processing bodies.
Cell cycle progression and transmitotic apoptosis resistance promote escape from extrinsic apoptosis
Summary: Cancer cells can escape extrinsic apoptosis by approaching mitosis, where transiently high Mcl-1 expression prevents caspase-triggered apoptosis execution.
Crosstalk between the calcineurin and cell wall integrity pathways prevents chitin overexpression in Candida albicans
Summary: The pathogenic fungus Candida can limit the overproduction of chitin in its cell wall to prevent loss of fitness via a calcineurin-dependent mechanism.
Summary: WTS-1, the C. elegans LATS homolog, synergistically maintains endosomal actin homeostasis by restraining PTRN-1-mediated F-actin assembly and facilitating UNC-60A-mediated F-actin disassembly, thereby sustaining endocytic recycling.
DUSP6 regulates radiosensitivity in glioblastoma by modulating the recruitment of phosphorylated DNAPKcs at DNA double-strand breaks
Summary: DUSP6 is upregulated in recurrent glioblastoma and has cytoplasmic and nuclear localization. DUSP6 inhibition radiosensitizes glioblastoma cells via impaired recruitment of p-DNAPKcs, increasing progression-free survival in vivo.
Highlighted Article: ALG2 is a regulating factor for STING trafficking from endoplasmic reticulum to perinuclear compartments, and Ca2+-binding ability is crucial for the function of ALG2 in this process.
Frizzled3 inhibits Vangl2–Prickle3 association to establish planar cell polarity in the vertebrate neural plate
Summary: Frizzled3 induces Vangl2 phosphorylation and promotes PCP in Xenopus neuroectoderm by inhibiting the formation of the anterior Vangl2–Prickle3 complex.
TOOLS AND RESOURCES
Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system
Highlighted Article: We established conditional knockout cell lines for Rab1 and Rab5, and found that loss of Rab1 induced perinuclear accumulation of early endosomes and that Rab5 was dispensable for lysosome biogenesis.
Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system
Summary: A genetically encoded phycocyanobilin biosynthesis system and plasmid series for use of phycocyanobilin as an iRFP chromophore enables bright near-infrared fluorescence imaging in fission yeast.