Issues
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Cover image
Cover Image
Cover: Three-dimensional reconstruction of a laboratory-generated human blood-vessel-on-a-chip. A human umbilical vein endothelial cell monolayer lines the vessel lumen, which is generated in a collagen matrix. Cell–cell junctions are labelled to show VE-cadherin in white, and nuclei are shown in blue. These vessels have a perfusable lumen lined with endothelial cells, forming a semi-permeable barrier. This system allows leukocyte transmigration to be studied in real time and in three dimensions. See article by A. C. I. van Steen, L. Kempers et al. (jcs258690).
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RESEARCH HIGHLIGHTS
FIRST PERSON
CELL SCIENTISTS TO WATCH
REVIEWS
Dynamic and cell-specific transport networks for intracellular copper ions
Summary: This Review describes accumulating evidence for diverse and cell-specific regulation of copper ion transporters that cells employ to adjust copper fluxes to their changing metabolic needs.
How cells tell up from down and stick together to construct multicellular tissues – interplay between apicobasal polarity and cell–cell adhesion
Summary: This Review introduces the main components of apicobasal polarity and cell–cell adhesion complexes and highlights the interdependence of these two networks in epithelia.
SHORT REPORT
A preferred sequence for organelle inheritance during polarized cell growth
Highlighted Article: Organelles are interconnected by contact sites, but they must be inherited from mother cells into buds during budding yeast mitosis. We report that this process occurs in a preferred sequence.
RESEARCH ARTICLES
Desmosome dualism – most of the junction is stable, but a plakophilin moiety is persistently dynamic
Highlighted Article: Desmosomes consist of a stable and a dynamic signalling moiety. This dualistic composition is maintained during adhesion maturation and after internalisation of whole desmosomes during cell scattering.
Fetuin-A secretion from β-cells leads to accumulation of macrophages in islets, aggravates inflammation and impairs insulin secretion
Summary: Fetuin-A secretion from β-cells promotes macrophage migration to the islets and also aggravates local inflammation in the islets, whereas secretions from M1 type macrophages impair insulin secretion.
Structural organization of the C1b projection within the ciliary central apparatus
Highlighted Article: We provide insight into the subunit organization and 3D structure of the C1b projection of the ciliary central apparatus and the mechanism by which it regulates dynein activity and ciliary beating.
The long non-coding RNA ET-20 mediates EMT by impairing desmosomes in breast cancer cells
Highlighted Article: The lncRNA ET-20, localized in antisense within the tenascin C gene, is upregulated during an EMT and in invasive breast cancer cell lines, and contributes to EMT by impairing desmosomes.
Proprotein convertase furina is required for heart development in zebrafish
Summary: A new layer of regulation of Notch1b proteins by Furina during zebrafish cardiac looping and trabeculation, and Furina-Notch1b axis mutations, provide new insight into human congenital heart disease.
PKC-ε regulates vesicle delivery and focal exocytosis for efficient IgG-mediated phagocytosis
Summary: Golgi-tethered PKC-ε regulates vesicle trafficking along microtubules and their fusion into the developing phagosome. Unexpectedly, the regulatory domain is sufficient for vesicle delivery.
The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair
Summary: De-ubiquitylating enzyme USP7 controls the HUWE1-induced negative feedback loop regulating signals within the Shoc2 signaling axis, and may play a role in the pathogenesis of NSLAH.
Dia- and Rok-dependent enrichment of capping proteins in a cortical region
Summary: Plus ends of actin filaments are enriched at cortical regions rich in Rho signaling in syncytial Drosophila embryos depending on the actin regulator Dia and Rho kinase.
His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation
Summary: Sorting of endocytic cargo to the multivesicular body is accompanied by endosomal maturation. Here, we provide a potential mechanism by which these two processes are linked.
FAM209 associates with DPY19L2, and is required for sperm acrosome biogenesis and fertility in mice
Summary: We report that a conserved novel gene, Fam209, is essential for sperm development in the mouse. The phenotype seen in the absence of Fam209 mimics that of defective sperm in some infertile men.
TOOLS AND RESOURCES
CZON-cutter – a CRISPR-Cas9 system for multiplexed organelle imaging in a simple unicellular alga
Summary: An engineered CRISPR-Cas9 system for simultaneous genome editing and organellar visualization in Cyanidioschyzon merolae can be used to investigate the function of any gene at organellar resolution.
Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix
Summary: Description of a functional hydrogel-based blood-vessel-on-a-chip model that enables detailed analysis of the complete leukocyte transendothelial migration process in real time.
CORRECTIONS
JCS Journal Meeting 2023: Imaging Cell Dynamics

Our 2023 Journal Meeting on ‘Imaging Cell Dynamics’ will be held from 14-17 May 2023 in Lisbon, Portugal. We have a limited number of spaces left so sign up now! Registration deadline: 31 March.
Call for papers: Cell and Tissue Polarity
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We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
Webinar: Increasing the visibility and impact of your research
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Would you like to increase the visibility and impact of your research and raise your profile internationally? If so, register for the very practical webinar we are running in association with HUBS on 23 February 2023.
Cell scientist to watch: Gautam Dey

We interviewed Gautam Dey, who became a group leader at EMBL in Heidelberg, Germany, in 2021. His lab investigates the fundamental organisational principles and evolutionary dynamics of the nuclear compartment across eukaryotes.
Mechanisms of eukaryotic transcription termination at a glance

Check out our latest Cell Science at a Glance article and accompanying poster for an overview of the current understanding about the mechanisms of transcription termination by the three eukaryotic RNAPs.