CELL SCIENTISTS TO WATCH
The Autophagy, Inflammation and Metabolism Center international eSymposium – an early-career investigators’ seminar series during the COVID-19 pandemic
Summary: We report on the Autophagy, Inflammation and Metabolism Center eSymposium 2020 for early career investigators (ECIs), describe its impact on ECI careers and provide a post-pandemic world meetings formula.
Downregulation of the Tem1 GTPase by Amn1 after cytokinesis involves both nuclear import and SCF-mediated degradation
Summary: The Tem1 GTPase, which is critical for mitotic exit and cytokinesis, is doubly inactivated at the end of mitosis by the Amn1 protein.
Tyrosine kinase-independent actions of DDR2 in tumor cells and cancer-associated fibroblasts influence tumor invasion, migration and metastasis
Summary: The collagen receptor DDR2, a receptor tyrosine kinase, exhibits tyrosine kinase-independent functions that influence paracrine regulation of breast tumor cell invasion and breast cancer metastasis.
A cell-based drug discovery assay identifies inhibition of cell stress responses as a new approach to treatment of epidermolysis bullosa simplex
Summary: High-content screening of an epidermolysis bullosa simplex cell model identifies inhibition of EGFR signalling as a mechanism that can reduce mutant keratin aggregate formation and improve intercellular cohesion.
The TPR- and J-domain-containing proteins DJC31 and DJC62 are involved in abiotic stress responses in Arabidopsis thaliana
Highlighted Article: DJC31 and DJC62 localize to the ER where they interact with cytosolic HSP70 and HSP90. Their loss in Arabidopsis results in sensitivity to osmotic stress and abscisic acid.
Fission yeast TOR complex 1 phosphorylates Psk1 through an evolutionarily conserved interaction mediated by the TOS motif
Summary: By analyzing S6 kinase in fission yeast, we have demonstrated that the TOR signaling (TOS) motif-mediated substrate recognition by TOR complex 1 is conserved from yeast to humans.
Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay
Summary: We describe an imaging-based assay to detect protein–protein interactions in fission yeast that can be used for cytosolic proteins, nuclear proteins and components of multiprotein complexes.
Cdc42 reactivation at growth sites is regulated by local cell-cycle-dependent loss of its GTPase-activating protein Rga4 in fission yeast
Highlighted Article: Resumption of polarized Cdc42 activation at growth sites depends on a cell-cycle cue. This is facilitated by the loss of a Cdc42 GAP, Rga4, from growth sites at the end of cell division.
Summary: The assembly of the AP1 adaptor complex is a highly orchestrated process controlled by AAGAB.
TOOLS AND RESOURCES
Highlighted Article: We demonstrate that ptychographic hard X-ray tomography is a powerful scanning tool that can image retina tissue at sub-200 nm resolution for analysis of neural degeneration processes.
Highlighted Article: We describe an assay that enables live-cell imaging of the autophagic turnover and degradation of inducible protein aggregates in conjugation with key players in the autophagy pathway.
Summary: An open-source Python library for accessing a range of hardware devices used in microscopy.