Issues
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Cover image
Cover Image
Cover: Stabilization of focal adhesions within an endothelial cell after combined inhibition of MAP4K4 and Arg by the tyrosine kinase inhibitor bosutinib. Integrin β1 is depicted in green, F-actin in purple and the nucleus is stained blue. See article by L. Botros et al. (jcs240077).
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RESEARCH HIGHLIGHTS
STICKY WICKETS
FIRST PERSON
CELL SCIENCE AT A GLANCE
The flipside of the TOR coin – TORC2 and plasma membrane homeostasis at a glance
Summary: A review of studies on target of rapamycin complex 2 in budding yeast suggesting that the primordial function of this kinase is to maintain the biophysical properties of the plasma membrane in a narrow, optimal window.
REVIEWS
Recent insights into peroxisome biogenesis and associated diseases
Summary: Peroxisome biogenesis and disease: VDAC2-BAK mediating Catalase export, a new mutation of Pex26 in PBD, a GTP provider DNNAMO1, and impaired BDNF-TrkB signaling in PBDs.
Non-caveolar caveolins – duties outside the caves
Summary: In this Review, we revisit the non-canonical roles of caveolins in a variety of cellular contexts including liver, brain, lymphocytes, cilia and cancer cells, as well as consider insights from invertebrate systems.
SHORT REPORT
Efficient genome editing by CRISPR-Mb3Cas12a in mice
Summary: CRISPR-Mb3Cas12a can target a broader range of sequences in murine zygotes compared to AsCas12a and LbCas12a, and has a lower frequency of large on-target insertions or deletions than seen with Cas9 and high overall knock-in efficiency.
RESEARCH ARTICLES
Actin waves transport RanGTP to the neurite tip to regulate non-centrosomal microtubules in neurons
Summary: The small G-protein Ran is an important non-centrosomal microtubule regulator in neurons, and the active GTP-bound Ran is transported by actin waves to its target region.
The microtubule end-binding affinity of EB1 is enhanced by a dimeric organization that is susceptible to phosphorylation
Summary: Phosphorylation of the linker domain causes EB1 to adopt a more curved structural conformation and disrupts the high-affinity binding of EB1 to microtubule growing ends.
Regulation of kinesin-1 activity by the Salmonella enterica effectors PipB2 and SifA
Summary: The kinesin-1 motor protein plays a key role in controlling the membrane exchanges necessary for the intracellular survival of Salmonella. The complexity of kinesin-1 regulation by Salmonella effector proteins is highlighted.
Sex differences in the proliferation of pulmonary artery endothelial cells: implications for plexiform arteriopathy
Summary: Human pulmonary artery endothelial cells from male and female donors differ in their proliferative potential, a finding that could explain the higher incidence of pulmonary arterial hypertension in women.
β1 integrin-mediated signaling regulates MT1-MMP phosphorylation to promote tumor cell invasion
Summary: Analysis of MT1-MMP phosphorylation and cell surface expression reveals a role for β1 integrin signaling during invadopodia formation and cell invasion.
Bosutinib prevents vascular leakage by reducing focal adhesion turnover and reinforcing junctional integrity
Summary: Combined inhibition of Arg and MAP4K4 by bosutinib reduces turnover of integrin-based focal adhesions. This provides robust protection against inflammation-induced vascular leakage.
CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division
Highlighted Article: CLIC4 regulates remodeling of the sub-plasma-membrane actomyosin network within the cytokinetic furrow by recruiting MST4 kinase and regulating ezrin phosphorylation, thus regulating cortex stiffness and blebbing.
EML4–ALK V3 oncogenic fusion proteins promote microtubule stabilization and accelerated migration through NEK9 and NEK7
Summary: EML4–ALK fusions are major drivers of lung cancer. Particular fusion variants act through a novel actionable pathway involving NEK7 and NEK9, promoting cell migration and potentially influencing metastatic progression.
Histone H1 eviction by the histone chaperone SET reduces cell survival following DNA damage
Highlighted Article: SET removes histone H1 from chromatin. When SET is depleted, the resulting reduction in histone H1 removal leads to enhanced resistance to various types of DNA lesions.
SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network
Summary: Nutrition starvation stress induces a spatiotemporal interaction of SNAP29 with the cytokeratin network, which mediates the assembly of CTP synthase filaments along the cytokeratin network to modulate enzymatic activity.
Evidence for macromolecular crowding as a direct apoptotic stimulus
Summary: Apoptosis was induced by osmotic shrinkage while keeping monovalent ions at fixed concentrations. It was found that shrinkage itself, rather than potassium loss or cytoskeleton-mediated effects, is responsible for apoptosis.
Induction of ligand promiscuity of αVβ3 integrin by mechanical force
Highlighted Article: Most integrins can bind several ligands. We show how one integrin, αVβ3, selects between two different ligands, fibronectin and vitronectin, based on force-regulated conformational changes in αVβ3 integrin.
5-Aza-2′-deoxycytidine advances the epithelial–mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements
Summary: Hypomethylation by 5-Aza-CdR upregulates the SIPA1 expression and promotes epithelial–mesenchymal transition in breast cancer cells, possibly increasing the risk of cancer cell metastasis.
A DNA polymerization-independent role for mitochondrial DNA polymerase I-like protein C in African trypanosomes
Highlighted Article: A dual functioning DNA polymerase in African trypanosomes supports DNA replication and distribution of mitochondrial DNA progeny.
Introducing our new Editors
We welcome three new Editors to Journal of Cell Science - Robert Parton, Richa Rikhy and Simon Cook. You can read more about them in the Editorial from our Editor-in-Chief Michael Way.
2024 Journal Meeting 'Diversity and Evolution in Cell Biology'
Registration is open for our 2024 Journal Meeting Diversity and Evolution in Cell Biology, which aims to bring together evolutionary biologists and cell biologists investigating diverse aspects of cellular physiology. Final registration deadline: 3 May 2024.
Workshop: Physics of the Early Embryonic Cell Divisions
Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place 11-14 November 2024. Application deadline: 17 May.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.
Propose a new Workshop for 2026
We are now accepting proposals for our 2026 Workshops programme. We aim to be responsive to the community and open to novel initiatives, so if you have a new idea for a biological workshop that you feel would work well, please apply. Applications deadline: 19 July 2024.