CELL SCIENCE AT A GLANCE
Summary: A review of studies on target of rapamycin complex 2 in budding yeast suggesting that the primordial function of this kinase is to maintain the biophysical properties of the plasma membrane in a narrow, optimal window.
Summary: Peroxisome biogenesis and disease: VDAC2-BAK mediating Catalase export, a new mutation of Pex26 in PBD, a GTP provider DNNAMO1, and impaired BDNF-TrkB signaling in PBDs.
Summary: In this Review, we revisit the non-canonical roles of caveolins in a variety of cellular contexts including liver, brain, lymphocytes, cilia and cancer cells, as well as consider insights from invertebrate systems.
Summary: CRISPR-Mb3Cas12a can target a broader range of sequences in murine zygotes compared to AsCas12a and LbCas12a, and has a lower frequency of large on-target insertions or deletions than seen with Cas9 and high overall knock-in efficiency.
Summary: The small G-protein Ran is an important non-centrosomal microtubule regulator in neurons, and the active GTP-bound Ran is transported by actin waves to its target region.
The microtubule end-binding affinity of EB1 is enhanced by a dimeric organization that is susceptible to phosphorylation
Summary: Phosphorylation of the linker domain causes EB1 to adopt a more curved structural conformation and disrupts the high-affinity binding of EB1 to microtubule growing ends.
Summary: The kinesin-1 motor protein plays a key role in controlling the membrane exchanges necessary for the intracellular survival of Salmonella. The complexity of kinesin-1 regulation by Salmonella effector proteins is highlighted.
Sex differences in the proliferation of pulmonary artery endothelial cells: implications for plexiform arteriopathy
Summary: Human pulmonary artery endothelial cells from male and female donors differ in their proliferative potential, a finding that could explain the higher incidence of pulmonary arterial hypertension in women.
Summary: Analysis of MT1-MMP phosphorylation and cell surface expression reveals a role for β1 integrin signaling during invadopodia formation and cell invasion.
Bosutinib prevents vascular leakage by reducing focal adhesion turnover and reinforcing junctional integrity
Summary: Combined inhibition of Arg and MAP4K4 by bosutinib reduces turnover of integrin-based focal adhesions. This provides robust protection against inflammation-induced vascular leakage.
CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division
Highlighted Article: CLIC4 regulates remodeling of the sub-plasma-membrane actomyosin network within the cytokinetic furrow by recruiting MST4 kinase and regulating ezrin phosphorylation, thus regulating cortex stiffness and blebbing.
EML4–ALK V3 oncogenic fusion proteins promote microtubule stabilization and accelerated migration through NEK9 and NEK7
Summary: EML4–ALK fusions are major drivers of lung cancer. Particular fusion variants act through a novel actionable pathway involving NEK7 and NEK9, promoting cell migration and potentially influencing metastatic progression.
Highlighted Article: SET removes histone H1 from chromatin. When SET is depleted, the resulting reduction in histone H1 removal leads to enhanced resistance to various types of DNA lesions.
SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network
Summary: Nutrition starvation stress induces a spatiotemporal interaction of SNAP29 with the cytokeratin network, which mediates the assembly of CTP synthase filaments along the cytokeratin network to modulate enzymatic activity.
Summary: Apoptosis was induced by osmotic shrinkage while keeping monovalent ions at fixed concentrations. It was found that shrinkage itself, rather than potassium loss or cytoskeleton-mediated effects, is responsible for apoptosis.
Highlighted Article: Most integrins can bind several ligands. We show how one integrin, αVβ3, selects between two different ligands, fibronectin and vitronectin, based on force-regulated conformational changes in αVβ3 integrin.
5-Aza-2′-deoxycytidine advances the epithelial–mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements
Summary: Hypomethylation by 5-Aza-CdR upregulates the SIPA1 expression and promotes epithelial–mesenchymal transition in breast cancer cells, possibly increasing the risk of cancer cell metastasis.
A DNA polymerization-independent role for mitochondrial DNA polymerase I-like protein C in African trypanosomes
Highlighted Article: A dual functioning DNA polymerase in African trypanosomes supports DNA replication and distribution of mitochondrial DNA progeny.