Issues

Summary: We discuss perspectives on the mechanisms of formation of Wnt- and Hedgehog-containing extracellular vesicles, and their functions in development and various human pathologies.

Summary: New structural and biophysical studies have shed much light on the physical mechanism of ESCRT-mediated membrane remodeling and scission.

Summary: This Review explores the emerging role of the biophysical properties of the basement membrane in regulating critical aspects of vascular health and disease.

Summary: In this Review, we highlight recent work identifying non-transcriptional mechanisms crucial to regulating the core mammalian clock proteins and their role in circadian rhythmicity.

Summary: In vitro F-actin gliding assays and TIRF microscopy reveal that myosin flattens the Arp2/3-dependent actin branches up to the point of them breaking, and reduces the probability that new branches are formed.

Summary: Complex phosphoregulation of the S. pombe contractile ring protein Fic1 influences polarity establishment and the transition from a unicellular state into a state with invasive pseudohyphal growth.

Summary: Cells remodel respiratory complex organization depending on metabolic status. Here, using a two-dimensional complexome profiling strategy, we propose a biphasic mechanism for this remodelling during proteostasis stress.

Summary: Investigation of intraflagellar transport in several trypanosome developmental stages extracted from tsetse flies provides new insights into mechanisms controlling flagellum length.

Highlighted Article: The β1 integrin interactor Rabgap1 promotes recycling of active β1 integrins from endosomes to enhance cell migration on 2D surfaces as well as cell invasion into a 3D matrix.

Summary: A generic Hox peptide works as an unconventional nuclear export signal (NES) to control Hox nuclear export and developmental autophagy in the Drosophila larval fat body.

Highlighted Article: Transitions in matrix stiffness create oscillatory patterns of endothelial monolayer barrier disruption and cell contractility.

Summary: Testing the targeting and tolerance of CRISPR effectors and gRNAs in mammalian cell lines towards the design of functional MitoCRISPR tools.

Highlighted Article: Using endogenous tagging and interaction proteomics, we identified the ER-associated lipid droplet biogenesis factor ACSL3 as a GABARAPL2-binding partner that serves as novel regulator of the enigmatic UFM1 conjugation pathway.

Summary: APC/C^Cdh1 ubiquitylates both borealin, via a non-canonical degron motif, and Aurora B to terminate activity of the CPC in G1 phase. Disruption of this ubiquitylation reduces the efficiency of DNA replication.

Summary: The non-fusogenic Sec22b–Stx1 SNARE complex contributes to plasma membrane expansion by interacting with the E-Syt lipid transfer proteins at ER–PM contact sites.

Summary: An imbalance of microtubule polyglutamylation leads to the disintegration of the posterior cytoskeleton and alterations of the swimming behaviour from a directional into a non-directional, diffusive mode in Trypanosoma brucei.

Summary: The mitochondrial fission protein dynamin-related protein 1 (DRP1) plays an essential role in early transcriptional reprogramming during beige and brown adipocyte differentiation.

Summary: A new methodology to synchronize RPE-1 cells in mitosis for large-scale biochemical and proteomics studies.