Cover: Epifluorescence image showing the Giardia lamblia microtubule cytoskeleton in a CRISPRi knockdown of the disc-associated protein DAP5188. The microtubule cytoskeleton, including the ventral disc, eight flagella and median body, is visualised in the knockdown strain through expression of mNeonGreen-tagged β-tubulin (green). See article by C. Nosala et al. (jcs227355).
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CELL SCIENCE AT A GLANCE
Membrane trafficking in the retinal pigment epithelium at a glance
Summary: This Cell Science at a Glance article focuses on membrane trafficking events in retinal pigment epithelial cells, which are uniquely specialised to fulfil the multiple functions of this epithelium in supporting vision.
Diverse cellular functions of barrier-to-autointegration factor and its roles in disease
Summary: BAF (BANF1) orchestrates key cellular processes including cell division, repair of nuclear ruptures, and anti-viral intrinsic immunity.
The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways
Summary: Transient interaction between RhoU and ITSN2 on a subset of vesicles regulates cargo recycling.
Disc-associated proteins mediate the unusual hyperstability of the ventral disc in Giardia lamblia
Highlighted Article: Identification of over 50 disc-associated proteins associated with the ventral disc in G. lamblia, two of which mediate microtubule hyperstability or limit microtubule dynamics.
Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1
Summary: CTR1 (SLC31A1) is the only known mammalian importer of copper. We show that CTR1 is a retromer complex cargo protein, and that retromer is required for cellular sensitivity to extracellular copper.
The lncRNA MEG3 mediates renal cell cancer progression by regulating ST3Gal1 transcription and EGFR sialylation
Summary: The lncRNA MEG3 mediates renal cell cancer progression. MEG3 regulates ST3Gal1 expression through interaction with the c-Jun transcription factor, with downstream effects on EGFR sialylation and PI3K–AKT signaling.
Lymphocytes perform reverse adhesive haptotaxis mediated by LFA-1 integrins
Summary: Like mesenchymal cells, lymphocytes perform adhesive haptotaxis, although adhesion is dispensable for amoeboid migration. Moreover, unlike mesenchymal cells, lymphocytes display a unique ability to follow decreasing gradients of adhesion.
The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment
Highlighted Article: An acidic patch in the kinetochore-targeting domain of CENP-E is required for association with the C terminus of BubR1 at kinetochores to facilitate chromosome alignment during mitosis.
BMPR1A is necessary for chondrogenesis and osteogenesis, whereas BMPR1B prevents hypertrophic differentiation
Summary: GDF5 mutants were used to investigate the role of BMPR1A and BMPR1B in chondrogenesis and osteogenesis. We found that BMPR1A is necessary for chondrogenesis and osteogenesis, and BMPR1B prevents hypertrophy.
The Na+ pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalization
Summary: Coincidence detection of phosphorylated and ubiquitylated cargo-specific regions is used for epsin target recognition among multiple ubiquitylated proteins in yeast.
TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis
Highlighted Article: ER transmembrane protein TMEM147 impacts on cellular cholesterol homeostasis via physical and functional interactions with LBR and DHCR, two key sterol reductases in cholesterol biosynthesis.
The structure and symmetry of the radial spoke protein complex in Chlamydomonas flagella
Highlighted Article: A 3D structure of the Chlamydomonas flagella radial spoke was obtained using single particle cryo-EM. Combined with cross-linking mass spectrometry and biophysics data, this allowed localization of components in the radial spoke head and neck regions.
Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast
Highlighted Article: The absence of Pex24 or Pex32, two Hansenula polymorpha ER proteins, disturbs peroxisome–ER contact sites and leads to multiple peroxisomal defects, which can be restored using an artificial tether protein.
JCS Journal Meeting 2023: Imaging Cell Dynamics
Our 2023 Journal Meeting on ‘Imaging Cell Dynamics’ will be held from 14-17 May 2023 in Lisbon, Portugal. Due to popular demand, we can currently only accept applications for online attendance. Apply now to attend this meeting virtually. Registration deadline: 31 March.
Call for papers: Cell and Tissue Polarity
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
Editorial: Publishing where it matters
Editor-in-Chief Michael Way outlines Journal of Cell Science’s plans for the upcoming year and introduces Seema Grewal as our new Executive Editor.
preLights 5th Birthday webinar
preLights, our preprint highlighting service, is celebrating its 5th birthday this year. To mark the occasion, join us online on 14 March 2023 at 16:00 GMT for a discussion, led by four preLights alumni, on how to identify and navigate the challenges and opportunities while shaping your career as an early-career researcher.
Cell Scientists to Watch
As a community-focused journal, Journal of Cell Science is keen to support the next generation of cell biologists. Check out Cell Scientists to Watch, our interview series featuring talented researchers who have recently set up their own labs.