Issues
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Cover image
Cover Image
Cover: Epifluorescence image showing the Giardia lamblia microtubule cytoskeleton in a CRISPRi knockdown of the disc-associated protein DAP5188. The microtubule cytoskeleton, including the ventral disc, eight flagella and median body, is visualised in the knockdown strain through expression of mNeonGreen-tagged β-tubulin (green). See article by C. Nosala et al. (jcs227355).
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RESEARCH HIGHLIGHTS
STICKY WICKET
FIRST PERSON
MEETING REPORT
CELL SCIENCE AT A GLANCE
Membrane trafficking in the retinal pigment epithelium at a glance
Summary: This Cell Science at a Glance article focuses on membrane trafficking events in retinal pigment epithelial cells, which are uniquely specialised to fulfil the multiple functions of this epithelium in supporting vision.
REVIEW
Diverse cellular functions of barrier-to-autointegration factor and its roles in disease
Summary: BAF (BANF1) orchestrates key cellular processes including cell division, repair of nuclear ruptures, and anti-viral intrinsic immunity.
RESEARCH ARTICLES
The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways
Summary: Transient interaction between RhoU and ITSN2 on a subset of vesicles regulates cargo recycling.
Disc-associated proteins mediate the unusual hyperstability of the ventral disc in Giardia lamblia
Highlighted Article: Identification of over 50 disc-associated proteins associated with the ventral disc in G. lamblia, two of which mediate microtubule hyperstability or limit microtubule dynamics.
Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1
Summary: CTR1 (SLC31A1) is the only known mammalian importer of copper. We show that CTR1 is a retromer complex cargo protein, and that retromer is required for cellular sensitivity to extracellular copper.
The lncRNA MEG3 mediates renal cell cancer progression by regulating ST3Gal1 transcription and EGFR sialylation
Summary: The lncRNA MEG3 mediates renal cell cancer progression. MEG3 regulates ST3Gal1 expression through interaction with the c-Jun transcription factor, with downstream effects on EGFR sialylation and PI3K–AKT signaling.
Lymphocytes perform reverse adhesive haptotaxis mediated by LFA-1 integrins
Summary: Like mesenchymal cells, lymphocytes perform adhesive haptotaxis, although adhesion is dispensable for amoeboid migration. Moreover, unlike mesenchymal cells, lymphocytes display a unique ability to follow decreasing gradients of adhesion.
The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment
Highlighted Article: An acidic patch in the kinetochore-targeting domain of CENP-E is required for association with the C terminus of BubR1 at kinetochores to facilitate chromosome alignment during mitosis.
BMPR1A is necessary for chondrogenesis and osteogenesis, whereas BMPR1B prevents hypertrophic differentiation
Summary: GDF5 mutants were used to investigate the role of BMPR1A and BMPR1B in chondrogenesis and osteogenesis. We found that BMPR1A is necessary for chondrogenesis and osteogenesis, and BMPR1B prevents hypertrophy.
The Na+ pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalization
Summary: Coincidence detection of phosphorylated and ubiquitylated cargo-specific regions is used for epsin target recognition among multiple ubiquitylated proteins in yeast.
TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis
Highlighted Article: ER transmembrane protein TMEM147 impacts on cellular cholesterol homeostasis via physical and functional interactions with LBR and DHCR, two key sterol reductases in cholesterol biosynthesis.
The structure and symmetry of the radial spoke protein complex in Chlamydomonas flagella
Highlighted Article: A 3D structure of the Chlamydomonas flagella radial spoke was obtained using single particle cryo-EM. Combined with cross-linking mass spectrometry and biophysics data, this allowed localization of components in the radial spoke head and neck regions.
Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast
Highlighted Article: The absence of Pex24 or Pex32, two Hansenula polymorpha ER proteins, disturbs peroxisome–ER contact sites and leads to multiple peroxisomal defects, which can be restored using an artificial tether protein.
Imaging Cell Architecture and Dynamics
We are still welcoming submissions for our upcoming Special Issue: Imaging Cell Architecture and Dynamics. This issue will be coordinated by two Guest Editors: Lucy Collinson (The Francis Crick Institute, UK) and Guillaume Jacquemet (University of Turku, Finland). Extended submission deadline: 29 March 2024.
Journal of Cell Science - more than just a journal
People who know JCS well will know that we're more than just a journal and that our community – the cell biology community – really is at the heart of everything we do. Read the full Editorial by Editor-in-Chief Michael Way and Executive Editor Seema Grewal.
2024 Journal Meeting 'Diversity and Evolution in Cell Biology'
Registration is open for our 2024 Journal Meeting Diversity and Evolution in Cell Biology, which aims to bring together evolutionary biologists and cell biologists investigating diverse aspects of cellular physiology. Submit your abstract by 5 April. Final registration deadline: 3 May 2024.
Workshop: Roles of Lipids in Nuclear Homeostasis and Genome Stability
Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place 14-17 October 2024. Application deadline: 19 April.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.