Issues

Summary: A summary of the various super-resolution microscopy techniques that are now part of the cell biologist's toolkit.

Summary: Nuclear myosins perform roles in critical processes such as transcription, genome organisation and DNA repair. We discuss how the biochemical and biophysical properties of myosins lend themselves to nuclear processes.

Summary: Clathrin-mediated endocytosis can be inhibited by mechanical factors leading to the formation of frustrated clathrin-coated structures that can fulfill new functions.

Summary: A novel inhibitory mechanism of glucocorticoid (GC) action on cell migration is dependent on the α-tubulin deacetylase HDAC6, which has implications for the design of novel GCs with improved therapeutic benefit.

Summary: Tumor-associated mutations of the lysosomal ubiquitin ligase RNF167-a could facilitate lysosomal exocytosis and might be responsible for plasma membrane repair in tumor cells.

Summary: Endothelial-secreted laminin-511 and α6 integrins promote endothelial morphogenesis by regulating expression of the chemokine receptor CXCR4. Depletion of α6 integrins from established tubes causes loss of tube integrity and laminin-511.

Summary: The flagellum attachment zone protein FAZ27 interacts with two flagellar proteins to maintain trypanosome cells in the trypomastigote form. Depletion of FAZ27 results in the formation of epimastigote-like cells.

Summary: The Cdc42 guanine-nucleotide-exchange factor Cdc24 interacts with septins during bud site assembly. This interaction assists in recruiting septins and stabilizing Cdc24 at the incipient bud site.

Summary: BMP4 is a novel prognostic factor for gastric cancer that modulates epithelial–mesenchymal transition to promote invasion and metastasis by increasing ID1 expression.

Highlighted Article: The small GTPase Rab32 localizes to lysosomes and supports cellular metabolism and growth as a novel regulator of the mechanistic target of rapamycin complex 1 (mTORC1).

Summary: Abl1 plays a crucial role in connecting Src family kinases to PI3K activation. Src/Hck-mediated Abl1 Tyr488 phosphorylation orchestrates PIK3CB signaling and the assembly of macrophage podosomes.

Summary: A simple and effective cell-free assay can be used for the detection, analysis and quantification of DNA repair mechanisms.

Highlighted Article: Chromosome segregation requires the spindle apparatus in both mitosis and meiosis. We report that kinesin-14 family proteins and microtubule dynamics function differently in mitosis and meiosis.

Summary: Centrosomal proteins regulate cell fate and are involved in ciliopathies. C3G, a protein essential for mammalian development, is a novel centrosomal protein that regulates centrosome duplication and primary cilium length.

Highlighted Article: Loss of Tetrahymena thermophila Poc5 reveals an important role for this centrin-binding protein in basal body maturation, which also impacts basal body production and ciliogenesis.

Summary: Osh6 transports phosphatidylserine to the plasma membrane. The membrane localization and lipid transfer activity of Osh6 is regulated by the tail region of the ER–PM tether protein Ist2.

Highlighted Article: γ-tubulin ring complexes are templates for microtubule nucleation and are composed of γ-tubulin and γ-tubulin complex proteins (GCPs). GCPs 4, 5 and 6 form a stable sub-complex that mediates assembly of the full complex.

Highlighted Article: A workflow using light-sheet microscopy to evaluate morphologic phenotypes in 3D cell cultures enables high-throughput studies of dynamic biological processes, target gene characterization and drug target candidate screens in vitro.