Cover: How to ship cells without dry-ice. Image shows a primary rat astrocyte fully recovered after 72 h of transit in agarose at ambient temperature. It is immunostained with anti-GFAP antibodies (green), and counterstained with Rhodamine–phalloidin, to highlight F-actin (red), and DAPI, to show the DNA (blue). See article by S. P. Wheatley and D. N. Wheatley (jcs238139).
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CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Nutrient regulation of mTORC1 at a glance
Summary: A summary of how extracellular and intracellular signals feed into the mTOR pathway, how the lysosome acts as an mTOR signaling hub, and how downstream signaling controls autophagy and lysosome biogenesis.
New insights into transcriptional reprogramming during cellular stress
Summary: A Review of recent developments in understanding the major cell-autonomous stress-responsive transcriptional regulatory pathways, including the activation of enhancers and complex changes in chromatin organization.
Rap1 and membrane lipids cooperatively recruit talin to trigger integrin activation
Summary: Talin requires cooperative Rap1 binding to its F0 and F1 domains and lipid binding to the F2 domain for its recruitment to adhesion sites to trigger integrin activation.
Reversible solidification of fission yeast cytoplasm after prolonged nutrient starvation
Summary: After prolonged quiescence, fission yeast cell populations switch state to immobilise subcellular components much more profoundly than cells experiencing acute energy depletion.
P-cadherin-induced decorin secretion is required for collagen fiber alignment and directional collective cell migration
Highlighted Article: P-cadherin-induced decorin expression allows collagen fiber alignment and subsequent activation of the β-PIX/Cdc42 axis to promote directional collective cell migration.
Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1
Highlighted Article: Phospho-regulation of the INCENP STD motif acts as a novel switch that is key to Aurora B activity modulation, chromosome alignment and cytokinesis.
Cul3 regulates cyclin E1 protein abundance via a degron located within the N-terminal region of cyclin E
Highlighted Article: Cyclin E is targeted by Cul3 for proteasomal degradation via a newly identified degron located within the N-terminal domain of cyclin E, a region that is often missing in cancer cells.
SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly
Summary: GRASP55 is highly acetylated on lysine 50 during mitosis, and its deacetylation by SIRT2 at mitotic exit promotes Golgi assembly through facilitating GRASP55 self-interaction.
TOOLS AND RESOURCES
Unintended perturbation of protein function using GFP nanobodies in human cells
Highlighted Article: Functionalized GFP-binding nanobodies (dongles) can extend the use of GFP-tagged proteins. Here, dongles that allow knocksideways are demonstrated, but they are limited by perturbation of the target protein caused by dongle binding alone.
Transporting cells over several days without dry-ice
Summary: An easy dry-ice-free method of sending cultured cells across the globe at ambient temperatures.
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Call for papers: Cell and Tissue Polarity
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FocalPlane Image Competition 2023
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