Issues

Summary: This Commentary documents recent advances concerning the two kinetochore-based force-generating mechanisms that drive mitotic chromosome congression in vertebrate cells.

Highlighted Article: Depending on the physical environment of the cell, the nuclear volume can change dramatically. Cytoskeletal filaments and motors are involved in regulating nuclear volume.

Summary: Obscurin is a titin-like protein in Drosophila muscle, which has two pseudokinase domains. These bind ligands essential for the correct assembly of the filament lattice.

Highlighted Article: The mRNA encoding certain tail-anchored proteins is directly localized to the surface of the endoplasmic reticulum, facilitating the insertion of newly synthesized proteins into the membrane.

Summary: The inwardly rectifying K⁺ channel KCNK1 negatively regulates osteoclast differentiation.

Highlighted Article: Chlamydia trachomatis is an obligate intracellular pathogen. Here, we describe how the Chlamydia virulence protein IPAM interacts with the host protein CEP170 to reprogram host MT organization.

Summary: Src-mediated phosphorylation on Y100 and Y1065 is a prerequisite for vinculin activation, and controls cytoskeletal mechanics by regulating force transmission between the actin cytoskeleton and focal adhesion proteins.

Highlighted Article: The EGFR ligand betacellulin contains a cytoplasmic basolateral-sorting motif. Mistrafficking of betacellulin, but not other EGFR ligands, induces EGFR-dependent lateral lumens in polarized MDCK cells.

Summary: It had previously been understood that Akt activation occurs solely at the plasma membrane. We show that PtdIns(3,4,5)P₃-dependent in situ activation of Akt can occur in subcellular compartments.

Highlighted Article: Interfering with lysine demethylase LSD1 function severely affects nuclear envelope and pore complex reformation at the end of mitosis, indicating that changes on the chromatin landscape regulate these processes.

Summary: The NMDA receptor and LRP1 function as co-receptors in Schwann cells, activating cell signaling in response to protein ligands and promoting cell survival and migration.

Summary: Src regulates the KCNQ2 channel through a dual rearrangement of cytosolic termini that underlies upregulation of single-channel gating and a reduction in the number of functional channels.

Summary: Mild caspase activation in cells subjected to non-lethal doses of stress generates an N-terminal p120 RasGAP fragment that inhibits NF-κB activity.