Polarized transport is essential for constructing multiple plasma membrane domains in the cell. Drosophila photoreceptors are an excellent model system to study the mechanisms of polarized transport. Rab11 is the key factor regulating the post-Golgi transport of rhodopsin 1 (Rh1), a photoreceptive protein, to the rhabdomere, a photoreceptive plasma membrane. Here, we found that neuronal Synaptobrevin (nSyb) colocalizes with Rab11 on the trans-side of Golgi stacks and post-Golgi vesicles at the rhabdomere-base, and nSyb-deficiency impairs rhabdomeric transport and induces accumulation of Rh1 and vesicles in the cytoplasm; this is similar to the effects of Rab11 loss. These results indicate that nSyb acts as a post-Golgi SNARE toward rhabdomeres. Surprisingly, in Rab11-, Rip11-, and nSyb-deficient photoreceptors, illumination enhances cytoplasmic accumulation of Rh1 colocalizing with Rab11, Rabenosyn5, nSyb, and Arrestin 1 (Arr1). Arr1 loss but not Rab5 dominant negative (Rab5DN) protein expression inhibits the light enhanced cytoplasmic Rh1 accumulation. Rab5DN inhibits the generation of Rh1 containing multi-vesicle bodies rather than Rh1 internalization. Overall, these results indicate that exocytic Rh1 mingle with endocytosed Rh1 and are then transported together to rhabdomeres.
Functions of neuronal Synaptobrevin in the post-Golgi transport of rhodopsin in Drosophila photoreceptors
These authors contributed equally to the work
- Award Group:
- Funder(s): Precursory Research for Embryonic Science and Technology
- Award Id(s): 25-J-J4215
- Funder(s):
- Award Group:
- Funder(s): Core Research for Organelle Diseases in Hiroshima University, Takeda Science Foundation, Ohsumi Frontier Science Foundation, Core Research for Evolutional Science and Technology
- Award Id(s): 15K07050, 19K06663
- Funder(s):
- Award Group:
- Funder(s): KAKENHI 508
- Award Id(s): 19K06566
- Funder(s):
- Award Group:
- Funder(s): JST SPRING
- Award Id(s): JPMJSP2132
- Funder(s):
- Award Group:
- Funder(s): Core Research for Evolutional Science and Technology
- Funder(s):
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Hitomi Yamashita, Yuka Ochi, Yumi Yamada, Shogo Sasaki, Tatsuya Tago, Takunori Satoh, Akiko K. Satoh; Functions of neuronal Synaptobrevin in the post-Golgi transport of rhodopsin in Drosophila photoreceptors. J Cell Sci 2022; jcs.260196. doi: https://doi.org/10.1242/jcs.260196
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