During the first cell cycles of the early development, the chromatin of the embryo is highly reprogrammed alongside that embryonic genome starts its own transcription. The spatial organization of the genome is a major process that contributes to regulating gene transcription in time and space, however, it is poorly studied in the context of early embryos. To study the cause and effect link between transcription and spatial organization in embryos, we focused on the ribosomal genes, that are first silent and begin to transcribe during the 2-cell stage in the mouse. We demonstrated that ribosomal sequences and early unprocessed rRNAs are spatially organized in a very peculiar manner from the 2-cell to the 16-cell. Using drugs interfering with ribosomal DNA transcription, we show that this organization, totally different from somatic cells, depends on an active transcription of ribosomal genes and induces a unique chromatin environment that favors transcription of major satellite sequences after the 4-cell stage.
Transcription of rRNA in early mouse embryos promotes chromatin reorganization and expression of major satellite repeats
The two authors contributed equally to the work.
Present address: Laboratoire Biologie et Santé, Equipe Endocrinologie et Biologie de la Reproduction animale Université Félix Houphouët - Boigny / BP V34, Abidjan 01.
Present address: Molecular Biology, Department of Pathology, Medical Teaching Institution, Lady Reading Hospital (LRH-MTI) Peshawar Pakistan.
Present address: CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France.
Present address: INRAE, PAO, 37380,Nouzilly, France.
Present address: Université Paris Saclay, INRAE, AgroParisTech, GABI, 78350 Jouy-en-Josas, France.
Present address: Univ Lyon, Université Claude Bernard Lyon 1, Inserm, INRA, Stem Cell and Brain Research Institute U1208, USC1361, 69500 Bron, France.
- Award Group:
- Funder(s): Agence Nationale de la Recherche
- Award Id(s): ANR-10-LABX-73
- Funder(s):
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Martine Chebrout, Maïmouna Coura Koné, Habib U. Jan, Marie Cournut, Martine Letheule, Renaud Fleurot, Tiphaine Aguirre-Lavin, Nathalie Peynot, Alice Jouneau, Nathalie Beaujean, Amélie Bonnet-Garnier; Transcription of rRNA in early mouse embryos promotes chromatin reorganization and expression of major satellite repeats. J Cell Sci 2022; jcs.258798. doi: https://doi.org/10.1242/jcs.258798
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