Mammalian PEX16 has been considered essential for generating and maintaining peroxisomal membranes. This view is based primarily on the finding that fibroblasts from several PEX16-deficient patients are devoid of peroxisomal structures, but can form peroxisomes upon expression of PEX16. However, unlike these patient-derived cells, pex16 mutants in other model organisms contain partially functional peroxisomes. Here, we report that PEX16-KO cells derived from three mammalian cultured cell lines are comprised of cells containing a fewer number of enlarged peroxisomes and cells lacking peroxisomes. We also suggest that PEX16 accelerates the process by which peroxisome-less cells form peroxisomal membranes and subsequently establish mature peroxisomes, independently on its ability to mediate peroxisomal targeting of PEX3. Nevertheless, PEX16 is not absolutely required for this process. Moreover, a well-known patient-derived PEX16 mutant inhibits the de novo formation of peroxisomal membranes. Our findings suggest that although PEX16 is undoubtedly important for optimal peroxisomal membrane biogenesis, mammalian cells may be able to form peroxisomes de novo and maintain the organelles without the aid of PEX16.
De novo formation and maintenance of mammalian peroxisomes in the absence of PEX16
Present address: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK
- Award Group:
- Funder(s): Japan Society for the Promotion of Science
- Award Id(s): JP26116007
- Funder(s):
- Award Group:
- Funder(s): Takeda Science Foundation
- Funder(s):
- Award Group:
- Funder(s): Naito Foundation
- Funder(s):
- Award Group:
- Funder(s): Japan Foundation for Applied Enzymology
- Funder(s):
- Award Group:
- Funder(s): Novartis Foundation (Japan) for the Promotion of Science
- Funder(s):
Currently Viewing Accepted Manuscript - Newer Version Available
- Split-screen
- Views Icon Views
- Open the PDF for in another window
-
Article Versions Icon
Versions
- Version of Record 09 May 2022
- Accepted Manuscript 19 April 2022
- Share Icon Share
-
Tools Icon
Tools
- Search Site
Yuichi Yagita, Yuichi Abe, Yukio Fujiki; De novo formation and maintenance of mammalian peroxisomes in the absence of PEX16. J Cell Sci 2022; jcs.258377. doi: https://doi.org/10.1242/jcs.258377
Download citation file:
Advertisement
Imaging Cell Architecture and Dynamics
We are still welcoming submissions for our upcoming Special Issue: Imaging Cell Architecture and Dynamics. This issue will be coordinated by two Guest Editors: Lucy Collinson (The Francis Crick Institute, UK) and Guillaume Jacquemet (University of Turku, Finland). Extended submission deadline: 29 March 2024.
Journal of Cell Science - more than just a journal
People who know JCS well will know that we're more than just a journal and that our community – the cell biology community – really is at the heart of everything we do. Read the full Editorial by Editor-in-Chief Michael Way and Executive Editor Seema Grewal.
2024 Journal Meeting 'Diversity and Evolution in Cell Biology'
Registration is open for our 2024 Journal Meeting Diversity and Evolution in Cell Biology, which aims to bring together evolutionary biologists and cell biologists investigating diverse aspects of cellular physiology. Submit your abstract by 5 April. Final registration deadline: 3 May 2024.
Workshop: Roles of Lipids in Nuclear Homeostasis and Genome Stability
Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place 14-17 October 2024. Application deadline: 19 April.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.