Sirtuin 2 (SIRT2) is an NAD-dependent sirtuin deacetylase that regulates microtubule and chromatin dynamics, gene expression, cell cycle progression as well as nuclear envelope reassembly. Recent proteomic analyses have identified Golgi proteins as SIRT2 interactors, indicating that SIRT2 may also play a role in Golgi structure formation. Here we show that SIRT2 depletion causes Golgi fragmentation and impairs Golgi reassembly at the end of mitosis. SIRT2 interacts with the Golgi reassembly stacking protein GRASP55 in mitosis when GRASP55 is highly acetylated on K50. Expression of wild-type and the K50R acetylation-deficient mutant of GRASP55, but not the K50Q acetylation-mimetic mutant, in GRASP55 and GRASP65 double knockout cells, rescued the Golgi structure and post-mitotic Golgi reassembly. Acetylation-deficient GRASP55 exhibited a higher self-interaction efficiency, a property required for Golgi structure formation. These results demonstrate that SIRT2 regulates Golgi structure by modulating GRASP55 acetylation levels.
SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly
Present address: Bio-Medical Center, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China
Currently Viewing Accepted Manuscript - Newer Version Available
Xiaoyan Zhang, Andreas Brachner, Eva Kukolj, Dea Slade, Yanzhuang Wang; SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly. J Cell Sci 2019; jcs.232389. doi: https://doi.org/10.1242/jcs.232389
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