While influenza kills about a half million people each year, even after excluding pandemics, there is only one set of antiviral drugs: neuraminidase inhibitors. Using a new approach utilizing giant unilamellar vesicles and infectious X-31 influenza virus and testing for the newly identified pore intermediate of membrane fusion, we observed ∼30 - 87 % poration as a function of lipid composition. Testing the hypothesis that spontaneous curvature (SC) of the lipid monolayer controls membrane poration, our Poisson model and Boltzmann energetic considerations suggest a transition from a leaky to a non-leaky fusion pathway depending on the SC of the target membrane. When the target membrane SC is below∼-0.20 nm−1 fusion between influenza virus and target membrane is predominantly non-leaky while above that fusion is predominantly leaky, suggesting that HA catalyzed topological conversion of target membranes during fusion is associated with a loss of membrane integrity.
Lipid-dependence of target membrane stability during influenza viral fusion
Currently Viewing Accepted Manuscript - Newer Version Available
Sourav Haldar, Elena Mekhedov, Chad D. McCormick, Paul S. Blank, Joshua Zimmerberg; Lipid-dependence of target membrane stability during influenza viral fusion. J Cell Sci 2018; jcs.218321. doi: https://doi.org/10.1242/jcs.218321
Download citation file:
Advertisement
Introducing our new Editors
We welcome three new Editors to Journal of Cell Science - Robert Parton, Richa Rikhy and Simon Cook. You can read more about them in the Editorial from our Editor-in-Chief Michael Way.
2024 Journal Meeting 'Diversity and Evolution in Cell Biology'
Registration is open for our 2024 Journal Meeting Diversity and Evolution in Cell Biology, which aims to bring together evolutionary biologists and cell biologists investigating diverse aspects of cellular physiology. Final registration deadline: 3 May 2024.
Workshop: Physics of the Early Embryonic Cell Divisions
Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place 11-14 November 2024. Application deadline: 17 May.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.
Propose a new Workshop for 2026
We are now accepting proposals for our 2026 Workshops programme. We aim to be responsive to the community and open to novel initiatives, so if you have a new idea for a biological workshop that you feel would work well, please apply. Applications deadline: 19 July 2024.