There was an error in J. Cell Sci. (2022) 135, jcs259046 ().
The article reported the identification of a novel temperature-sensitive fission yeast mutant pkd2-B42 and its characterization that revealed the function of Pkd2 in cell growth. In it, the coding sequence of the pkd2 gene in this mutant was determined, and the authors identified eight missense mutations at the time (Fig. 1A; Table S1).
However, the authors now report that there is an additional insertion mutation in the pkd2 coding sequence of pkd2-B42, which was missed in the previous sequencing reactions. The insertion of a single guanine nucleotide (G) at the 2020 bp position of the coding sequence also changes the pkd2 sequence from GGA to GGGA in pkd2-B42. This results in two additional missense mutations at the amino acid residues of 675 and 676, followed by a premature stop codon, in the open reading frame of pkd2-B42.
The authors have informed us that these additional mutations do not change the nature of pkd2-B42 as a temperature-sensitive mutant, nor change the phenotype of this pkd2 mutant (because the number of mutations in pkd2-B42 is irrelevant to its genetic and cell biology characterization described in the study). Therefore, the authors conclude that this new finding does not change any of the conclusions reached in the paper.
The correct Fig. 1A is shown below, and has been updated in the PDF and online versions. Table S1 has also been updated to include these additional mutations:
A novel temperature-sensitive pkd2 mutant, pkd2-B42. (A) Schematic of the predicted topology of wild-type Pkd2p. The mutated residues in pkd2-B42 are shown as red asterisks. The length is 676 amino acids owing to a truncation of the C-terminus. Numbers denote the nine transmembrane helices.
A novel temperature-sensitive pkd2 mutant, pkd2-B42. (A) Schematic of the predicted topology of wild-type Pkd2p. The mutated residues in pkd2-B42 are shown as red asterisks. The length is 676 amino acids owing to a truncation of the C-terminus. Numbers denote the nine transmembrane helices.
A novel temperature-sensitive pkd2 mutant, pkd2-B42. (A) Schematic of the predicted topology of wild-type Pkd2p. The mutated residues in pkd2-B42 are shown as red asterisks. Numbers denote the nine transmembrane helices.
The authors apologise for this error and any inconvenience caused.
