ABSTRACT
Immune responses involve many types of leukocytes that traffic to the site of injury, recognize the insult and respond appropriately. Imaging of the immune system involves a set of methods and analytical tools that are used to visualize immune responses at the cellular and molecular level as they occur in real time. We will review recent and emerging technological advances in optical imaging, and their application to understanding the molecular and cellular responses of neutrophils, macrophages and lymphocytes. Optical live-cell imaging provides deep mechanistic insights at the molecular, cellular, tissue and organism levels. Live-cell imaging can capture quantitative information in real time at subcellular resolution with minimal phototoxicity and repeatedly in the same living cells or in accessible tissues of the living organism. Advanced FRET probes allow tracking signaling events in live cells. Light-sheet microscopy allows for deeper tissue penetration in optically clear samples, enriching our understanding of the higher-level organization of the immune response. Super-resolution microscopy offers insights into compartmentalized signaling at a resolution beyond the diffraction limit, approaching single-molecule resolution. This Review provides a current perspective on live-cell imaging in vitro and in vivo with a focus on the assessment of the immune system.
Introduction
The complexity of the immune system makes the imaging of various immune cells as a whole demanding but also worthwhile. Although non-optical methods, such as positron emission tomography (PET) and magnetic resonance imaging (MRI) exist (Willmann et al., 2008), their resolution is not at the single-cell level. Hence, this Review will be limited to optical approaches with higher resolution that have been developed in the last few years. We will place the focus on the understanding of immune cells and their signaling at the subcellular and molecular level using new and emerging microscopic techniques. The starting point for this Review are three recent reviews that cover multiphoton and confocal live-cell imaging (Germain et al., 2012; McArdle et al., 2016; Pittet et al., 2018). We will cover advances in imaging of the immune system towards a subcellular and molecular understanding through the application of total internal reflection fluorescence (TIRF) and quantitative dynamic footprinting (qDF) microscopy, Förster resonance energy transfer (FRET) imaging, intravital imaging, light-sheet microscopy (LSM) and super-resolution microscopy.
Imaging methods
TIRF and qDF microscopy
The contact region between a cell and its substrate is of great biological importance. A large number of molecular events occur at the cellular surface, where cells exchange materials and information with the surrounding environment. Examples include leukocyte rolling, adhesion (Ley et al., 2007), ligand–receptor interaction, T cell receptor (TCR) engagement (Dustin and Choudhuri, 2016), molecule transport, and exocytotic and endocytotic processes (Wu et al., 2014). TIRF microscopy is a technique that is best suited to study these membrane-associated processes at the cell–surface interface. Thus, TIRF is a high-resolution live-cell imaging method. However, TIRF is not suitable for in vivo studies, because it requires a glass–cell interface that does not exist in vivo.
TIRF microscopy was first introduced by Daniel Axelrod (Axelrod, 1981). It allows visualization of cell and substrate contacts within a thin region of a specimen (∼200 nm from a coverslip). This is achieved by selectively illuminating and exciting fluorophores in a thin layer of the specimen in the immediate vicinity of the glass–water interface using an evanescent wave. The evanescent wave is generated when the incident light is totally reflected at the glass–water interface, because the electromagnetic fields cannot be discontinuous. The critical angle is the smallest angle of incidence that yields total reflection. The main purpose of TIRF in biology is to look at only the cell surface at the glass–water interface. TIRF completely eliminates the background fluorescence further into the cell.
One of the evolved versions of TIRF is based on variable angle TIRF (Stock et al., 2003), also called quantitative dynamic footprinting (qDF) (Fig. 1A) (Sundd et al., 2012, 2010). qDF was developed to image the footprints of live leukocytes rolling, adhering, spreading or migrating on a glass coverslip. qDF is diffraction-limited in the x- and y-axes, but affords single-digit nanometer resolution in the z-axis. Reasonable temporal resolution of better than one frame per second (fps) has been achieved (Sundd et al., 2012, 2010). Up to three fluorochromes have been imaged simultaneously (Fan et al., 2016; Sundd et al., 2011), thus allowing for simultaneous analysis of three molecular events. qDF is suitable for imaging small 3D membrane structures, such as microvilli, tethers and slings in living immune cells (Sundd et al., 2012, 2010). qDF has also been combined with super-resolution microscopy to achieve high resolution in x, y and z planes, showing that T cell receptors (TCRs) are concentrated in the microvilli of T cells (Jung et al., 2016). However, unlike qDF, super-resolution microscopy usually entails fixed specimens and long data collection times, which are unfavorable for live-cell imaging.
qDF combined with a homogeneous binding assay (Chigaev and Sklar, 2011) allows for reporting of dynamic antibody binding to molecules in the membrane of live cells. For example, antibodies reporting the conformation of integrin β2 have been introduced into flow chambers to show β2 integrin activation in live cells that move under shear stress (Fan et al., 2016). Here, exposure of human neutrophils to the chemokine IL-8 induces a β2 integrin conformation shift to a high-affinity form as reported by an activation-specific antibody (Fig. 1). qDF imaging revealed that β2 integrins can achieve this high-affinity conformation by first extending and then rearranging the β2 I-like domain (the canonical pathway of integrin activation) or by attaining high affinity first and extending subsequently (Fan et al., 2016). qDF is also suitable to track fluorescent protein (FP) reporter constructs to map proteins near the plasma membrane. Indeed, we (L.W. and K.L.) are currently using FP-fused kindlin-3 to analyze its recruitment to the plasma membrane during neutrophil rolling and arrest.
FRET imaging
FRET requires (1) a substantial overlap (more than 30%) between the emission spectrum of the donor and the excitation spectrum of the acceptor; (2) that both excitation spectra must be separated enough to allow independent excitation and photobleaching (Fig. 2A); and (3) that the donor and acceptor fluorophores must be in a favorable spatial orientation and close proximity. Cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) are an established pair of donor and acceptor fluorescent proteins (Bajar et al., 2016; Lam et al., 2012). They can be engineered into the same protein, and so can also serve as force probes (Grashoff et al., 2010) or probes for biochemical events (O'Shaughnessy et al., 2019).
FRET is outstanding in probing subcellular signaling because it can measure signaling with high spatial and temporal resolution in live cells. FRET can be performed with almost any type of fluorescent microscope. It works deep in the tissue, at low numerical aperture, and even in flow cytometry, where immune cells ‘fly’ by the excitation laser and detection photomultiplier tubes (PMTs) in a small fraction of a second (Fan et al., 2016; Lefort et al., 2009; Stadtmann et al., 2013). FRET multiphoton microscopy permits intravital imaging with deep tissue penetration and represents an emerging area of in vivo analysis of signaling events under physiological conditions (Pittet and Weissleder, 2011; Radbruch et al., 2015).
The methods to quantify the FRET efficiency include primarily (1) fluorescence intensity-based analysis, (2) fluorescence intensity-independent lifetime analysis, and (3) anisotropy-based analysis (Shrestha et al., 2015). Thus, FRET efficiency can be detected as a ratio (for example, FRET:CFP) or shortening of the donor fluorescence lifetime in FLIM analysis (Shrestha et al., 2015). Based on practical considerations, some general approaches have demonstrated their potential in studying biological samples, including two-color emission ratio imaging, acceptor photobleaching and FLIM-FRET (Box 1).
Two-color emission ratio imaging, also known as sensitized emission
This is a popular method applied to probing protein–protein interactions or intramolecular biosensing in both microscopy and flow cytometry. Samples are illuminated at the donor excitation wavelength, and fluorescence is collected in both donor and acceptor (FRET) channels. The fluorescence intensity of the acceptor channel divided by the donor channel is defined as the FRET index. Ratiometric imaging can be practiced on a regular wide-field or confocal fluorescence microscope. Since the ratiometric imaging is intensity dependent, separating FRET from non-FRET signal can often be a major challenge. Controls and corrections for fluorescence cross-talk, bleed-through and photobleaching are required. A constant ratio of the donor to acceptor concentrations is important for reliable ratiometric FRET imaging. In an intramolecular FRET biosensor, the sensing domain is sandwiched by the donor and acceptor fluorophores. Thus, the expression level of donor to acceptor fluorophores is perfectly 1:1. One good example is the cGMP biosensor (see Fig. 2B) (Russwurm et al., 2007).
Acceptor photobleaching
This method measures increased donor emission when the acceptor is photobleached. Acceptor photobleaching is based on destroying acceptor fluorophores, which naturally abolishes FRET. After acceptor photobleaching, the donor intensity increases, since no FRET energy is transferred to the acceptor. This method is therefore straightforward, but destructive (single-time measurement) and thus not suitable for capturing molecular dynamics.
FLIM-FRET
The fluorescence lifetime (τ) is defined as the average time that a molecule remains in an excited state prior to emitting a photon and returning to the ground state. The presence of acceptor molecules within the local environment of the donor that permits energy transfer will influence the fluorescence lifetime of the donor. By measuring the donor lifetime in the presence and the absence of acceptor one can estimate the distance between the donor- and acceptor-labeled proteins.
FRET has also been used to investigate integrin activation. A spatial separation of the integrin αL and β2-subunit cytoplasmic tail has been detected during activation of K562 myeloid cells transfected with integrin αL–CFP and integrin β2–YFP (Kim et al., 2003). Here, acceptor photobleaching FRET was performed to assess the conformational changes that are reflected by the distance between the cytoplasmic tails of the two integrin subunits. In the resting state, these domains were found close to each other. A loss of FRET upon integrin activation was interpreted as spatial separation between integrin αL and β2 (Kim et al., 2003).
The formation of the immune synapse during antigen presentation is another area that has been intensely investigated using FRET methods. The adaptive immune response is initiated when the TCR is engaged by antigenic peptides bound by major histocompatibility complex (pMHC) proteins that are expressed on the surface of antigen-presenting cells (APCs). The interface between an APC and a lymphocyte is called immunological synapse (or immune synapse). The TCR–pMHC complex is surrounded by co-stimulatory and adhesion molecules on the membranes of both cells (Dustin and Choudhuri, 2016). Using flow cytometry-based FRET assays with fluorescein isothiocyanate (FITC) and tetramethylrhodamine (TRITC) as FRET pairs and conjugated monoclonal antibodies against the surface molecules [i.e. cluster of differentiation 3 (CD3), CD4 and CD45], FRET was detected between the CD3–TCR co-receptor complex and CD4, but not between CD3–TCR and CD45 when CD4+ T cells were activated using anti-CD3 monoclonal antibodies (mAbs) (Mittler et al., 1989). These experiments demonstrated that CD4 molecules on the surface of a murine T-cell hybridoma rapidly associate with the murine TCR–CD3 complex after crosslinking of the TCR complex with anti-CD3 mAbs. By using flow cytometric analysis to measure FRET between fluorochrome-labeled CD4 and CD3 molecules, it was determined that the association of the two receptors requires CD3 crosslinking and is dependent on the expression of the cytoplasmic domain of the CD4 molecule (Collins et al., 1992). It was later shown that the movement of CD3 to CD4 further depends on the tyrosine kinase Lck and the cytoplasmic domain of CD4 (Collins et al., 1992; Philipsen et al., 2017; Stirnweiss et al., 2013). These data are consistent with the idea that CD4 plays an active role in CD3-mediated signal transduction in T cells, whereas CD45 has an inhibitory role in TCR signaling (Dustin and Choudhuri, 2016).
Both acceptor photobleaching and ratiometric FRET were applied to investigate the association between CD3ζ and CD4 in the context of agonist or antagonist binding (Zal et al., 2002). Unlike photobleaching FRET microscopy, the ratiometric approach is compatible with 3D and time-lapse experiments. In this study, CFP (donor) was fused with CD3ζ, and YFP (acceptor) was fused to CD4. The authors detected an increase of FRET efficiency between CD3ζ–CFP and CD4–YFP in response to the agonist peptide presentation by MHC II. Thus, recognition of agonist pMHC complexes triggers an intermolecular interaction between CD4 and TCR, which is detectable across the T-hybridoma–APC contact area (Zal et al., 2002).
FLIM-FRET has been used to measure TCR clustering (Ma et al., 2017); to that end, the authors first developed membrane-anchored, single-molecule FRET pairs that underwent intermolecular FRET at high molecular density. This cluster sensor (named CliF) utilized the sensitivity of FRET to detect membrane protein clusters irrespective of the presence of non-clustered molecules. FLIM-FRET measured the increased clustering of TCR–CD3ζ upon T cell activation, which was correlated with cluster density (Ma et al., 2017).
One primary application of FRET is to use intramolecular biosensors to monitor activities of signaling molecules in cells in vivo. An emerging trend is to do this using intravital imaging; this has been facilitated by recent advances in the development of improved FRET biosensors with high FRET efficiency, good specificity and excellent response kinetics (Bajar et al., 2016; Greenwald et al., 2018; Lam et al., 2012). Mice have been engineered to express FRET-based biosensors, and intravital FRET imaging has been applied to study signaling molecules, such as cGMP (Thunemann et al., 2013; Wen et al., 2018), cAMP (Sprenger et al., 2015), Ca2+ (Yang et al., 2018), protein kinase A (PKA) and extracellular signal-regulated kinase 1/2 (ERK1/2) activity (Mizuno et al., 2014).
Confocal and multiphoton microscopy for intravital imaging
Studying immune cells in their native environment is the key benefit of intravital imaging. Intravital imaging is a very old technique, going back to the invention of the microscope (van Leeuwenhoek, 1719), but major advances included the introduction of fluorescence microscopy around 1980 (Sanderson et al., 2014), confocal microscopy (Minsky, 1988) and multiphoton microscopy (Larson, 2011). These methods are now well established and are commercially available from many manufacturers, and will not be covered here. Limitations include finite tissue transparency, light absorption and scattering, and the trauma associated with surgical access (McArdle et al., 2016). The use of intravital imaging to monitor cellular behavior has already been reviewed extensively (Germain et al., 2012; McArdle et al., 2016; Pittet et al., 2018). We will focus in this section on the advances towards understanding the molecular signaling by using biosensors.
One type of biosensing system based on single-color fluorescent proteins has been developed to detect molecular signaling events, such as Ca2+ mobilization (Zariwala et al., 2012). The GFP-based Ca2+ indicator GCaMP6 has been used to monitor T cell activation in vivo (Moseman et al., 2016). The single fluorescence-based method is easy to use, can generate very good signals and has good time resolution. However, it is sensitive to tissue movement and focal plane changes; thus, imaging must be performed on a relatively static object. In comparison, two-color ratiometric FRET imaging has many advantages, because the artifacts caused by tissue movement can be removed. For example, FRET-based Ca2+ biosensors have allowed high resolution functional tracking of T lymphocytes (Thestrup et al., 2014).
Two-photon FRET microscopy has been used to visualize activity changes of protein kinases in neutrophils during neutrophil recruitment to the inflamed intestine of transgenic mice expressing FRET biosensors that contained CFP and YFP (Mizuno et al., 2014). Ratiometric FRET imaging showed that ERK1/2 activity increased during neutrophil recruitment to the inflamed intestine in vivo, while high PKA activity suppressed neutrophil migration (Mizuno et al., 2014).
However, even in mechanically stable preparations, the tissue through which both excitation and emission signals must travel can cause problems because of absorption, scattering and autofluorescence. Because transmission of light with longer wavelengths (like YFP) in biological tissues is higher than that of light with shorter wavelength (like CFP), the apparent FRET efficiency calculated by the conventional ratiometric method becomes higher when the emitted light originates from deeper tissues. In addition, tissue autofluorescence undermines the net FRET efficiency (Sebestyén et al., 2002). Both problems can be solved by assessing FRET with FLIM. Because the lifetime of a fluorescence protein does not rely on the fluorescence intensity, the measurement is not affected by the transmission or light scattering in non-uniform environments. The detection of lifetime is accurate as long as the emitted light remains detectable at the PMT.
The FLIM system is compatible with multiphoton microscopy (Becker, 2012). In biosensor mice for the small GTPase RhoA (RhoA-FRET), FLIM-FRET imaging has been performed in highly motile cells, such as neutrophils. RhoA activity was monitored in swarming neutrophils in vivo in a laser ablation-induced tissue damage model in the mouse ear (Nobis et al., 2017). For each neutrophil, the RhoA activity was mapped from the front to the rear of the cell, relative to the direction of motion, indicating that RhoA is activated during neutrophil migration. Similar FRET-based biosensor mice have also been used for the evaluation of drug targeting efficacy in vivo (Nobis et al., 2017, 2018, 2013). The downside of FLIM-FRET is the lack of speed (more than 20 s per section) (Becker, 2012).
Light-sheet microscopy
LSM (Huisken et al., 2004; Mickoleit et al., 2014; Scherz et al., 2008; Siedentopf and Zsigmondy, 1902) offers relief from the light toxicity, low tissue penetration and close working distance of conventional confocal microscopy. LSM became practical with the advent of fast charge-coupled device (CCD) cameras (Huisken et al., 2004). While LSM is widely used for cleared tissues (Dodt et al., 2007), it is also suitable for live-cell imaging in transparent organisms and tissues, such as zebrafish, embryos or the mouse eye. Unlike other types of microscopy, the excitation is usually generated by a continuous wave laser and a cylindrical lens (Buytaert et al., 2012; Buytaert and Dirckx, 2009; Santi et al., 2009), and the excitation and emission pathways in LSM are orthogonal to each other. The resolution of LSM is diffraction limited, but acquisition is extremely fast (tens of milliseconds), thus limiting light toxicity and allowing high frame rates. LSM evolved to digitally scanned LSM with the use of a Gaussian laser beam (Keller et al., 2008), which enabled using two-photon excitation that is suitable for imaging deeper tissues (Truong et al., 2011). Four-lens systems have been developed to improve registration (Krzic et al., 2012). In addition, structured illumination reduced out-of-focus light (Huisken and Stainier, 2007).
A typical application of LSM in live-cell imaging is tracking genetically fluorescent cells in the zebrafish heart (Ding et al., 2017; Lee et al., 2016; Packard et al., 2017). Cell tracking is useful for studying development, inflammation and immunity. LSM has demonstrated the dynamics of cardiac valves during the cardiac cycle (Lee et al., 2013). Using a rainbow fluorescent protein system, the source of new cardiomyocytes was identified in mice at birth (Sereti et al., 2018). LSM is inherently 3D, allowing the imaging of an entire mouse heart in 60 s. Another study has demonstrated vascular calcification in the Apoe−/− mouse model of atherosclerosis (Hsu et al., 2018). Lungs and eyes have also been imaged by LSM. For example, LSM was employed to characterize fungal growth and the local immune response at cellular resolution in the context of whole lungs (Amich et al., 2020). LSM has not been used in live mice except in the eye, which is naturally clear (Prahst et al., 2019 preprint). More recently, LSM has been utilized for live-cell imaging of signaling events in cells transfected with a FRET biosensor for activity of the small GTPase Rap1; this allowed for a visualization of the rapid 3D dynamics of Rap1 in vesicles and cell protrusions (O'Shaughnessy et al., 2019).
Recent developments in LSM include adaptive optics to compensate for distortions (Liu et al., 2018a; Masson et al., 2015; Wilding et al., 2016), and the use of two-photon Bessel beams for single-cell imaging by LSM (Planchon et al., 2011). A very recent development is the advent of single-objective LSM (Millett-Sikking et al.; https://github.com/AndrewGYork/high_na_single_objective_lightsheet/tree/0.2.1). Unlike the conventional LSM, the single-objective LSM provides compatibility with standard confocal microscopy, better sample accessibility, reduced phototoxicity and improved volumetric imaging performance.
Super-resolution microscopy
Fluorescence microscopy is a powerful tool for imaging immune cells. However, its spatial resolution is limited by the physics of diffraction. This limitation is caused by the optical spot size in the lateral dimensions (in xy, d=λ/2NA), which is determined by the wavelength of the light being observed (λ) and the numerical aperture (NA) of the objective. In conventional fluorescence microscopy, the best lateral resolution is ∼200–300 nm. The extent of the focal spot is even larger (≥500 nm, and often more) in the axial direction (in z).
The diffraction limit was overcome by the invention of multiple microscopic techniques (Fig. 3), such as stimulated emission depletion (STED) (Hell and Wichmann, 1994; Klar and Hell, 1999; Klar et al., 2000), structured illumination microscopy (SIM) (Gustafsson, 2000; Heintzmann and Cremer, 1999), single-molecule localization microscopy (SMLM), which includes stochastic optical reconstruction microscopy (STORM) (Rust et al., 2006), photo-activated localization microscopy (PALM) (Betzig, 1995; Betzig et al., 2006; Dickson et al., 1997; Hess et al., 2006), points accumulation for imaging in nanoscale topography (PAINT) (Sharonov and Hochstrasser, 2006), and expansion microscopy (ExM) (Chen et al., 2015). In the past few years, STED, SIM, STORM and PALM super-resolution microscopes have all become commercially available and thus have been extensively used to investigate the distribution and function of different molecules found on a variety of leukocytes.
Most studies published to-date apply super-resolution microscopy to fixed samples, but here, we focus on super-resolution imaging of live-cell samples (Cox, 2015; Godin et al., 2014). The major challenge in super-resolution live-cell imaging are speed, buffer and light toxicity. In super-resolution imaging (∼50 nm resolution), cells, cell processes or tracked molecules that move faster than 50 nm per acquisition frame will not be imaged accurately. Thus, faster super-resolution imaging techniques, such as SIM (at least 9.3 Hz) (Turcotte et al., 2019), Airyscan (30 Hz) (Huff, 2016) and STED (12.5 Hz for 2.9×2.9 μm) (Yang et al., 2014), are more suitable for live-cell imaging. SIM is the most commonly used super-resolution imaging technique for live-cell imaging (Chen et al., 2018; Fiolka et al., 2012; Turcotte et al., 2019). SIM has a good imaging speed, can use any labels used in conventional live-cell imaging, and has tolerable light toxicity, similar to conventional live-cell imaging. Airyscan is also widely used for live-cell imaging (Kolossov et al., 2018; Mishra et al., 2019; Scipioni et al., 2018) and here has similar properties compared to SIM. The limitation of both SIM and Airyscan is that the improvement in resolution compared to confocal imaging is small (less than a factor of two). Thus, SIM and Airyscan are at the lower end of super-resolution imaging. STED is attractive for live-cell imaging, because it has much better resolution (70 to 90 nm) compared to SIM and Airyscan. Accordingly, STED has been used in multiple live-cell imaging studies (Bottanelli and Schroeder, 2018; Heine et al., 2018; Kilian et al., 2018; Mishina and Belousov, 2017). However, the major limitation of STED live-cell imaging is phototoxicity. To some extent, this can be improved by adjusting the imaging conditions (Kilian et al., 2018). SMLM has the highest resolution and has been used in studies of live-cell imaging, for instance STORM (Jones et al., 2011; Shim et al., 2012), PALM (Deschout et al., 2016; Shroff et al., 2008), PAINT (Giannone et al., 2013). However, at least 1000 frames of raw data are required for the reconstruction of a single frame of the image. Thus, the temporal resolution is limited as one fluorescent molecule may generate multiple different localizations within one image. Live-cell STORM imaging is also limited by probes that can ‘blink’ in physiological media, because STORM buffer is toxic for live cells. A few dyes such as DiI, Mito-Tracker, ER-Tracker and Lyso-Tracker have been used in live-cell STORM (Shim et al., 2012). PALM is commonly used for single-molecule tracking in live cells (Katz et al., 2017; Katz et al., 2019), which can show the tracks of the sparse photo-activated molecules, but not all labeled molecules. Often, the surrounding structures are acquired using diffraction-limited microscopy (Katz et al., 2017; Katz et al., 2019). Phototoxicity is a problem for live-cell STORM and PALM imaging, as STORM needs a high-power laser, while PALM uses a 405 nm laser to trigger the photoactivable fluorescent proteins.
Overall, super-resolution microscopy techniques are able to break the diffraction limit to improve the resolution by a factor of two to ten. By using super-resolution imaging, investigators can thus visualize small structures of the cell organelles and cytoskeleton in more detail. Combining STORM with molecular modeling has achieved single-molecule resolution for integrins, including the distribution of molecule nanoclusters, co-localizations and spatial distribution patterns (Fan et al., 2019).
Immune cells imaged by super-resolution microscopy
Super-resolution microscopy has been used in studying the functions and molecular distributions of different immune cells, and has provided molecular details of many biological processes, including neutrophil recruitment (Fan et al., 2016; Kolaczkowska and Kubes, 2013; Ley et al., 2018; Margraf et al., 2019; Mócsai et al., 2015), exocytosis (Cassatella et al., 2019; Johnson et al., 2016b; Ley et al., 2018; Mollinedo, 2019), phagocytosis (Colucci-Guyon et al., 2011; Davies et al., 2019; Kambara et al., 2018; Manfredi et al., 2018; Matlung et al., 2018; Nordenfelt and Tapper, 2011) and NETosis, which involves the release of neutrophil extracellular traps (NETs) (Ali et al., 2019; Clark et al., 2007; Hsu et al., 2019; Manfredi et al., 2018; Neubert et al., 2018; Perdomo et al., 2019; Sollberger et al., 2018).
A recent study used STORM to investigate the spatial distribution and reorganization of CD44 (a selectin ligand) expressed on KG1a cells, an acute myelogenous leukemia cell line similar to neutrophil/monocyte progenitors, during their rolling under shear stresses of 1 dyn cm−2 (AbuZineh et al., 2018). Their results suggest that the spatial reorganization of CD44 and the actin cytoskeleton is the result of the concerted effect of E-selectin–ligand interactions, external shear stress and spatial clustering of the selectin ligands (AbuZineh et al., 2018).
The transition of rolling to arrest is an important step for immune cell recruitment. In neutrophils, β2 integrins undergo two major conformational changes during activation (Fan and Ley, 2015): the resting state has a bent extracellular domain and low-affinity headpiece (denoted E−H−). In the canonical switchblade model (Lefort and Ley, 2012; Luo et al., 2007; Moore et al., 2018; Nordenfelt et al., 2016, 2017) of integrin activation, an E−H− integrin will extend first (denoted E+H−), and then acquire the high-affinity conformation (denoted E+H+). Alternative models, such as the deadbolt model have been suggested. In this model, the β-tail domain CD loop regulates the H+ regardless of E+ (Arnaout et al., 2007; Gupta et al., 2007). By using qDF microscopy with conformation-reporting antibodies, we observed an integrin conformation with a bent extracellular domain and a high-affinity headpiece (E−H+) and its transition to the E+H+ conformation on primary live neutrophils (Fan et al., 2016). Furthermore, by combining molecular modeling-based spatial simulation and STORM, we recently developed a technique called SuperSTORM, which allowed us to visualize integrin activation at the single-molecule level and to identify the number, distribution and arrangement (clustering) of three active conformations (E+H−, E−H+ and E+H+) of β2 integrins (Fan et al., 2019).
Another study addressed the role of the tetraspanin CD37 in neutrophil arrest and showed that CD37 regulates β2 integrin-mediated adhesion and migration in neutrophils (Wee et al., 2015). STORM imaging revealed that CD37 and the β2 integrin do not co-cluster significantly in human neutrophils. Combined with their observation of actin polymerization and Rac-1 activation deficiencies in CD37-knockout cells, the authors conclude that CD37 promotes neutrophil adhesion and recruitment by facilitating cytoskeletal functions downstream of integrin-mediated adhesion (Wee et al., 2015).
Real-time SIM imaging revealed the release of exosomes (CD63–GFP) as vesicular trails from cells during the initial stages of migration (Szatmary et al., 2017), which may release leukotriene B4 to boost the recruitment of other neutrophils. SIM has also been used to image the actin cytoskeleton of HL-60 cells (Gustafsson et al., 2008; Millius and Weiner, 2009) and neutrophils (Tanaka et al., 2017), as well as to determine the distribution of ADP-ribosylation factor 1 (ARF1) and Ras-related C3 botulinum toxin substrate 1 (Rac1) during HL-60 migration (Mazaki et al., 2017), the distribution of Rac1 and RhoA during neutrophil migration (Tanaka et al., 2017), and interactions between neutrophils and platelets (Bennewitz et al., 2017).
Super-resolution STORM imaging has been used to study neutrophil endosome maturation (He et al., 2016), exocytosis (Johnson et al., 2016a) and migration (Ramadass et al., 2019). Munc13-4 associated with syntaxin 7, indicating a role of Munc13-4 in regulating late endosomal maturation, endosomal signaling and Toll-like receptor 9 (TLR9)-initiated cellular responses (He et al., 2016). STORM imaging also showed that Munc13-4 is associated with the small GTPase Rab11; thus, Munc13-4 can regulate the trafficking and docking of Rab11-positive vesicles at the cell membrane, which is critical for the killing of bacteria by neutrophils (Johnson et al., 2016a). The trafficking protein synaptotagmin-like 1 (SYTL1, also known as JCF1) appears to regulate the localization of Rac1-GFP during neutrophil chemotaxis, which is important for neutrophil recruitment during inflammation or infections (Ramadass et al., 2019).
During NETosis, neutrophils can release three-dimensional meshworks (NETs) of chromatin (Brinkmann et al., 2004). Super-resolution STED microscopy has recently been used to image chromatin during the late stage of neutrophil NETosis (P2, before rupture) (Neubert et al., 2018). However, the authors observed no differences in NET structures between STED imaging and confocal imaging, from which they concluded that the chromatin mesh size is below the resolution of STED (i.e. 120 nm as claimed by the authors) (Neubert et al., 2018). In another study, SIM imaging was used to show that NETs can trap human immunodeficiency virus-1 (HIV-1) (Saitoh et al., 2012). This prevents HIV-1 from spreading and ensures high local concentrations of antiviral factors to eliminate HIV-1 (Saitoh et al., 2012). SIM imaging was also used to show the presence of myeloperoxidase (MPO), citrullinated histone H3 (H3Cit), protein arginine deiminases 4 (PAD4) and histone H2A–H2B in NET-forming neutrophils in samples from patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis, which is an unusual autoimmune disease caused by the release of MPO by neutrophils as an autoantigen in glomeruli (O'Sullivan et al., 2015). Populations of glycolipids change markedly during leukocyte differentiation, suggesting that these molecules are involved in biological functions. STED analysis showed that the lipid-raft marker phosphatidylglucoside and the glycosphingolipid lactosylceramide form different domains on plasma membranes of neutrophils (Ekyalongo et al., 2015), with phosphatidylglucoside preferentially expressed along the neutrophil differentiation pathway.
Different super-resolution techniques (SIM, STED and SMLM), have been used to image and compare microtubules in Drosophila macrophages (Wegel et al., 2016). All three techniques resolved well-separated microtubules, but not microtubule bundles (Wegel et al., 2016). Using super-resolution microscopy, the formation of podosome-like structures during Fc receptor-mediated phagocytosis could be observed (Ostrowski et al., 2019). Unlike conventional podosomes, these structures are short-lived and vectorial, expanding radially from the sites where phagocytic targets are initially engaged. Time-lapse SIM of phagocytosis of cultured microglia cells revealed different phases of macrophage phagocytic behavior, including initial active internalization, final digestion and autophagy (Fumagalli et al., 2019). Phagocytosis of Porphyromonas gingivalis by RAW 264.7 macrophages has also been assessed by SIM (Lam et al., 2016; Lenzo et al., 2016), showing that M1 and M2 macrophages have higher phagocytic capacity than naïve macrophages. Imaging of F-actin in wild-type (WT) and in myosin IXb (Myo9b)-knockout macrophages by SIM showed the loss of actin polarization in Myo9b-knockout cells and demonstrated the importance of Myo9b in regulating actin distribution and cell migration (Hanley et al., 2010). Another study also used SIM to image F-actin in WT and Cdc42-knockout macrophages, and showed that the defect in cell spreading is due to Cdc42 deficiency (Horsthemke et al., 2017). SIM was also used to evaluate the expression and distribution of ionized Ca2+-binding adaptor molecule 1 in macrophages in the cochlea, suggesting that the human auditory nerve is under the surveillance and possible neurotrophic stimulation of a well-developed resident macrophage system (Liu et al., 2018b). SIM also revealed that tunneling nanotubes (TNTs), membranous channels that connect cells, allow for the transfer of signals, vesicles and organelles (Hanna et al., 2017). Extracellular vesicles derived from oligodeoxynucleotide-stimulated macrophages can transfer and activate Cdc42 in the recipient cells and enhance immune responses (Zhang et al., 2019). SMLM imaging showed the distribution and clustering of Toll-like receptor 4 (TLR4) molecules on macrophages (Aaron et al., 2012; Kruger et al., 2017; Neumann et al., 2019). Lipopolysaccharide (LPS) stimulation increased the size of TLR4 clusters (Aaron et al., 2012). STORM imaging revealed nanoclusters of signal regulatory protein α (SIRPα), Fc gamma receptor I (FcγRI) and Fc gamma receptor II (FcγRII) on human macrophages (Lopes et al., 2017), and β2 integrins on human neutrophils (Fan et al., 2019). Nanoclusters of FcγRI, but not FcγRII, are constitutively associated with nanoclusters of SIRPα. Upon Fc receptor activation, Src family kinase signaling leads to segregation of FcγRI and SIRPα nanoclusters. The formation of cholesterol crystals in J774A.1 macrophage-like cells has also been followed by STORM imaging (Varsano et al., 2018). With regard to viral infection, PALM and STORM imaging have been used to visualize the process from cytoplasmic entry to HIV-1 to the integration of the reverse transcribed genome in macrophages (Peng et al., 2014). STED microscopy was used to identify cholesterol microdomains on the macrophage membrane, which can shed excess cholesterol (Jin et al., 2018). STED also showed the transfer of HIV-1 from macrophages to astrocytes (Russell et al., 2017). A STORM study showed that the number of histone H2A clusters in the nucleus is decreased in macrophages upon LPS stimulation (Nair et al., 2018), suggesting that histones may be released into the circulation by macrophages and promote distal inflammatory responses.
Of all immune cells, the imaging of lymphocytes is the most challenging, because (resting) lymphocytes are much smaller than other immune cells. Super-resolution microscopy has provided a superior means to investigate the function and regulation of lymphocytes, especially T lymphocytes. Using PALM imaging, the spatio-temporal dynamics of TCR complexes and linker for activation of T cells (Lat), a key adaptor molecule in the TCR signaling pathway, was determined in T cell membranes (Lillemeier et al., 2010). In quiescent T cells, both molecules existed in separate membrane domains (so-called protein islands) and these domains concatenated after T cell activation (Lillemeier et al., 2010). Their follow-up study provided evidence that TCR and CD4 molecules are clustered separately in resting T cells (Roh et al., 2015). Upon activation, TCR and CD4 begin to cluster together and develop into microclusters that then undergo a larger-scale redistribution to form supramolecular activation clusters (Roh et al., 2015). Another study also directly observed the organization of TCRs and their rearrangement on lymph node-resident T cells during an immune response by using STORM and SIM (Hu et al., 2016). They found that TCRs were preclustered into nanometer-scale protein islands, which were organized into larger membrane territories, and that T-cell activation induced the formation of microclusters (Hu et al., 2016). Using PALM-based single-molecule tracking, a cycle of recruitment, activation and release for Zeta-chain-associated protein kinase 70 (ZAP70) kinases was discovered at phosphorylated TCRs (Katz et al., 2017). Another study used STORM imaging to address the recruitment and phosphorylation of TCRζ, ZAP70, Lat and SLP76 (SH2-domain-containing leukocyte protein of 76 kDa), a key orchestrator of TCR signal transduction, in Jurkat T cells that were spread on CD3 antibody-coated substrate (Soares et al., 2013). A recent study challenged the view that TCRs are preclustered by using label-density-variation SMLM and STED (Rossboth et al., 2018); their findings suggest that TCRs are instead randomly distributed on the plasma membrane of resting antigen-experienced T cells (Rossboth et al., 2018). However, this interpretation is not fully supported by the images, which showed clustered TCRs in resting T cells and clustering disappeared after cells had spread. In non-spreading T cells, STORM combined with variable-angle total internal reflection microscopy showed that their TCRs are highly concentrated on microvilli (Jung et al., 2016). The authors interpreted this to mean that the immunological synapse may start to form at microvilli.
Similar to what was found in neutrophils as described above (Fan et al., 2019), integrin molecules are also important for the migration of T lymphocytes. Interferometric PALM and a constrained photoactivatable fluorescent protein fused to αLβ2 integrin was used to measure the displacement of the head of integrin LFA-1, as a result of its conformational change that is induced on the surface of migrating Jurkat T cells (Moore et al., 2018).
Recording cytoskeletal actin dynamics during the activation of resting T cells by STED microscopy (Fritzsche et al., 2017) showed that, following contact formation with activating surfaces, these cells sequentially rearrange their cortical actin across the entire cell, creating a previously unreported ramifying actin network above the immunological synapse. This network shows all the characteristics of an inward-growing transportation network and its dynamics correlate with T cell receptor rearrangements. SIM imaging has revealed the cortical actin and tubulin networks in cytotoxic T lymphocytes (CTLs) (Ritter et al., 2015). A super-resolution optical fluctuation imaging technique has been used to assess the CD4 clusters on the plasma membrane of Jurkat T cells (Lukeš et al., 2017). STORM imaging was also used to follow the infection of HIV to T cells (Roy et al., 2013). Finally, some super-resolution microscopy studies have focused on B lymphocytes. STORM identified B cell antigen receptor (BCR) microclusters on B cells (Maity et al., 2015). SIM and STORM revealed the distribution of the cytoskeletal protein vimentin in B cells (Tsui et al., 2018).
Concluding remarks
Imaging the immune system at the cell level has provided abundant information of how immune cells traffic and interact. Here, we highlight three key developments. With the advent of advanced FRET probes, it has become possible to image signaling events in live cells in real time. Deeper tissue penetration, 3D reconstruction and lower light toxicity has been achieved by light-sheet microscopy. Finally, imaging immune responses at the molecular level in subcellular domains and in real time is now on the horizon with the advent of various super-resolution approaches that are compatible with live-cell imaging. Further improvements in live-cell imaging by super-resolution microscopy will require more sensitive and faster detectors to reduce the light toxicity and increase acquisition speed.
Acknowledgements
We apologize to the authors whose work could not be cited because of space constraints.
Footnotes
Funding
Our work in this area was supported by grants from the National Institutes of Health, USA (HL078784 to K.L., R01HL145454 to Z.F.), a Postdoctoral Fellowship (19POST34450228 to L.W.) and a Career Development Award (18CDA34110426 to Z.F.) from the American Heart Association, USA. Deposited in PMC for release after 12 months.
References
Competing interests
The authors declare no competing or financial interests.