The vesicle coat protein COPI is at the heart of trafficking through the Golgi, but its precise roles are still the subject of some debate. The recruitment of COPI involves ADP-ribosylation factors (ARFs), which cycle from inactive to active states with the help of ARF-activating guanine nucleotide exchange factors (GEFs). On p. 3929, Elizabeth Sztul and colleagues investigate the role of COPI in Golgi structure and function by knocking down the ARF-GEF GBF1. They show that GBF1 is the major recruiter of COPI in the cell. Surprisingly, preventing recruitment of COPI to membranes does not cause the complete collapse of the secretory pathway but does cause selective tubulation of the cis-Golgi. Equally unexpected is the finding that, although depletion of GBF1 blocks the transport of transmembrane proteins, it does not affect the trafficking of soluble secretory proteins. The authors therefore suggest that GBF1-mediated COPI recruitment is required for trafficking of select types of cargo – transmembrane proteins in particular – through specific stages of the secretory pathway.
COPIng with selection
COPIng with selection. J Cell Sci 15 November 2007; 120 (22): e2205. doi:
Download citation file:
Advertisement
Cited by
Introducing our new Editors
We welcome three new Editors to Journal of Cell Science - Robert Parton, Richa Rikhy and Simon Cook. You can read more about them in the Editorial from our Editor-in-Chief Michael Way.
2024 Journal Meeting 'Diversity and Evolution in Cell Biology'
Registration is open for our 2024 Journal Meeting Diversity and Evolution in Cell Biology, which aims to bring together evolutionary biologists and cell biologists investigating diverse aspects of cellular physiology. Final registration deadline: 3 May 2024.
Workshop: Physics of the Early Embryonic Cell Divisions
Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place 11-14 November 2024. Application deadline: 17 May.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.
Propose a new Workshop for 2026
We are now accepting proposals for our 2026 Workshops programme. We aim to be responsive to the community and open to novel initiatives, so if you have a new idea for a biological workshop that you feel would work well, please apply. Applications deadline: 19 July 2024.