Export of secretory cargo from the ER occurs in vesicles bearing the COPII coat complex. Increasing evidence indicates that the process is regulated by phosphorylation. However, the protein kinases involved have remained unclear. Now, on p. 3839, David Stephens and colleagues report that PCTAIREs - a branch of the cyclin-dependent kinase family - interact directly with COPII and modulate cargo transport. To identify regulators of COPII function, they used two-hybrid screening with the human COPII subunit Sec23 as bait. One positive clone encoded a fragment of PCTAIRE-3, one of three PCTAIRE isoforms. The authors then used co-immunoprecipitation studies in cell lysates to show that recombinant PCTAIRE-1 and PCTAIRE-3 can both interact with Sec23. Finally, they demonstrate that specifically inhibiting PCTAIRE kinase activity causes gross changes in the organization and function of the early secretory pathway. Stephens and colleagues therefore conclude that PCTAIRE plays a role in this pathway by regulating COPII function.
Traffic police: PC TAIRE and COPII
Traffic police: PC TAIRE and COPII. J Cell Sci 1 September 2005; 118 (17): e1705. doi:
Download citation file:
Advertisement
Cited by
Reasons to submit to Journal of Cell Science

There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.
Call for papers: Cell and Tissue Polarity
-PolarityCFP.png?versionId=5161)
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
The Forest of Biologists

The Forest of Biologists is a biodiversity initiative created by The Company of Biologists, with support from the Woodland Trust. For every Research and Review article published in Journal of Cell Science a native tree is planted in a UK forest. In addition to this we are protecting and restoring ancient woodland and are dedicating these trees to our peer reviewers. Visit our virtual forest to learn more.
Propose a new Workshop for 2025

Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
FocalPlane Image Competition 2023

FocalPlane’s 2023 image competition is underway, in celebration of the community site’s third birthday. Click here for submission details and information on how to showcase your favourite image.