... reduced the number of B1a cells, MZBs, ILC2s and Tregs, both in situ and upon transplantation, indicating an intrinsic and essential role for IL7Rα. Surprisingly, reciprocal transplants of wild-type HSCs showed that an IL7Rα −/− environment selectively impaired reconstitution of TLCs when compared...

... demonstrated by the induction of the striatal transcription factors CTIP2, GSX2 and FOXP2. Crucially, these human LGE progenitors readily differentiate into postmitotic neurons expressing the striatal projection neuron signature marker DARPP32, both in culture and following transplantation in the adult...

... month,some cells express MBP. ( K ) Three months after transplantation, the grafted cells, as revealed by hNA and MBP, preferentially localize in the corpus callosum (CC, outlined). ( L ) All of the human cells are positive for OLIG2. ( M ) The human cells exhibit multiple MBP + processes. ( N...

... and generated offspring following spermatogonial transplantation into the testes of infertile recipient mice, indicating that they are functionally normal. These results demonstrate that activation of the PI3K-Akt pathway plays a central role in the self-renewal division of spermatogonial stem cells. Studies...

... is sufficient to induce a fully functional testicular soma capable of supporting complete spermatogenesis in the XX body. Here, we demonstrate that the testicular somatic environment of XX/Sry males is defective in supporting the later phases of spermatogenesis. Spermatogonial transplantation analyses using XX...

... a single progenitor to theoretically form sufficient cells for∼4×10 7 adult brains. AHNPs longitudinally maintain the ability to generate both glial and neuronal cell types in vivo and in vitro,and are amenable to genetic modification and transplantation. These findings suggest an unprecedented degree...

..., and the maturation process, from epiblast cells through PGCs to postnatal spermatogonia, can occur in the postnatal testicular environment. Primordial germ cell transplantation techniques will also provide a novel tool to assess the developmental potential of PGCs, such as those manipulated in vitro or recovered...

.... We used two cell labels, wheat germ agglutinin gold conjugates(WGA-Au) ( Smits-van Prooije et al.,1986 ; Chan and Tam,1988 ; Trainor and Tam,1995 ) and DiI, to label the premigratory neural crest by direct microinjection or by orthotopic transplantation of a fragment of labelled neural crest tissue...

... BCRP1/ABCG2 Transplantation Spermatogenesis is a step-wise process that requires coordination of mitosis, meiosis and differentiation. Numerous knockout and spontaneously mutated murine models exhibit altered spermatogenesis, underscoring the complexity of homeostatic control in this lineage...

...Anne Holz; Barbara Bossinger; Thomas Strasser; Wilfried Janning; Robert Klapper As in many other organisms, the blood of Drosophila consists of several types of hemocytes, which originate from the mesoderm. By lineage analyses of transplanted cells, we specified two separate anlagen that give rise...

... -1349. Sadaghiani, B. and Theibaud, C. H. ( 1987 ). Neural crest development in the Xenopus laevis embryo, studies by interspecific transplantation and scanning electron microscopy. Dev. Biol . 124 , 91 -110. Saldivar, J., Krull, C., Krumlauf, R., Ariza-McNaughton, L. and Bronner-Fraser...

...Robert Klapper; Sandra Heuser; Thomas Strasser; Wilfried Janning In order to reveal syncytia within the visceral musculature of Drosophila melanogaster , we have combined the GAL4/UAS system with the single-cell transplantation technique. After transplantation of single cells from UAS-GFP donor...

... from an early stage (Stainier,D. Y. R., Weinstein, B. M., Detrich, H. W. R., Zon, L. I. and Fishman, M. C. (1995) Development 121, 3141-3150). cloche mutants lack endocardium, as well as head and trunk endothelium, and nearly all blood cells. Cell transplantation studies have revealed...

...@sci.himeji-tech.ac.jp ) 27 10 1999 27 11 1999 © 1999 by Company of Biologists 1999 Regeneration Blastema formation Dorsoventral interaction Planarian Chimera Transplantation The planarian is remarkable in its ability to regenerate. Even a small fragment cut from...

... Transplantation Vasculogenesis Yolk sac The chorioallantoic placenta of eutherian mammals is critical for fetal development and growth during gestation. While much effort has been devoted to investigating the roles of the mature placenta, study of differentiation of two of its component structures...

...Edith C. Kordon; Gilbert H. Smith ABSTRACT Any epithelial portion of a normal mouse mammary gland can reproduce an entire functional gland when transplanted into an epithelium-free mammary fat pad. Mouse mammary hyperplasias and tumors are clonal dominant populations and probably represent...

... at the rostral and caudal extremities of the graft to indicate the limits of the transplant after healing. For somite grafting, the somites were dissected surgically from a quail donor and grafted in a slit performed lateral to r4-6 or r1-3. The operated embryos were fixed in 4% formaldehyde : 2 Mm EGTA...

... by transplantation of midline cells as well as by homotopic expression of single-minded , the master gene for midline development. Furthermore, ectopic midline cells are able to induce enhanced expression of some lateral CNS cell markers. We thus conclude that the CNS midline plays an important role...

..., which is expressed in the ventricular zone, in cortical development. Embryos homozygous for a mutation of Pax-6 (Small eye; Sey) had abnormalities suggesting defective migration of late-born cortical precursors. When late-born Sey/Sey precursors were transplanted into wild-type embryonic rat cortex...

... hematopoietic recovery in lethally irradiated mice transplanted with purified Thy-1.1lo Lin −Sca-1+ hematopoietic stem cells . Blood 83 , 3758 – 79 . 10.1182/blood.V83.12.3758.3758 Uchida , N. and Weissman , I. L. ( 1992 ). Searching for hematopoietic stem cells: evidence that Thy...