...), no antibody (lane 4) and normal rabbit IgG (lane 5). Fig. 5. Neurod1 and p27Kip1 are direct Cux2 targets in the embryonic nervous system. Complexes isolated from E12.5 mouse brains were immunoprecipitated with either anti-Cux2 or anti-RNA polymerase II antibodies followed by reverse cross-linking of protein...

.... † Author for correspondence (e-mail: nsegil@hei.org ) * These authors contributed equally to this work 9 5 2006 © 2006. 2006 p27Kip1 Cell cycle Development Morphogenesis Inner ear Cochlea Organ of Corti Mouse Cell proliferation and differentiation are coordinated...

... -297. Chen, P. and Segil, N. ( 1999 ). p27(Kip1) links cell proliferation to morphogenesis in the developing organ of Corti. Development 126 , 1581 -1590. Cheng, M., Sexl, V., Sherr, C. J. and Roussel, M. F. ( 1998 ). Assembly of cyclin D-dependent kinase and titration of p27Kip1 regulated...

... primordium in the cochlea is marked by p27Kip1 and the zone of non-proliferating cells (ZNPC). Timed-mated pregnant animals were injected with BrdU at E12.5 (A,B), E13.5 (C,D) and E14.5 (E,F), and sacrificed 8 hours later. Adjacent sections from each stage were stained with antibody to either BrdU (A,C,E...

... for wild type). (C,D) Percentages of cells expressing cyclin A (C) and p27kip1 (D). Wild-type cells show no significant change in the number of cells expressing cyclin A and p27kip1 over the culture period (r2=0.07 for wild-type data in C and 0.22 for wild-type data in D). There is a significant decrease...

... the number of cell divisions to growth arrest and differentiation. A direct prediction of this model is that an increase in the number of cell divisions would result in a delayed onset of differentiation. Since the cell cycle inhibitor p27Kip1 is an essential component of the machinery leading...

... . 10.1126/science.280.5366.1069 Durand , B. , Fero , M. L. , Roberts , J. M. and Raff , M. C. ( 1998 ). p27Kip1 alters the response of cells to mitogen and is part of a cell-intrinsic timer that arrests the cell cycle and initiates differentiation . Curr. Biology 8 , 431...