...Rocco J. Rotello; Pierre-Alain Fernandez; Junying Yuan ABSTRACT We have isolated a group of monoclonal antibodies that specifically recognize either apoptotic or engulfment cells in the interdigit areas of chicken hind limb foot plates, and throughout the embryo. Ten of these antibodies (anti...

.... , Vaudaux , P. E. and Skriver , L. ( 1979 ). Cytoskeletal proteins of the cell surface in Tetrahymena . I. Identification and localization of major proteins . Expl Cell Res . 123 , 311 – 320 . 10.1016/0014-4827(79)90473-7 cortical differentiation monoclonal antibodies protein...

... of monoclonal antibodies (Mabs) specific for the C μ and C v heavy Ig chain isotypes and for the light chain constituents shared by IgM and IgY molecules. Clusters of IgM- and of IgY-synthesizing lymphocytes, often located in separate sites, are first present in spleen sections of 7-week-old 25 mm larvae, about...

...Ian E. Crandall; Peter C. Newell ABSTRACT We have produced a series of monoclonal antibodies that recognize carbohydrate epitopes on cell surface glycoproteins of developing amoebae of Dictyostelium discoideum . The antibodies were found to have differential specificity for amoebae at different...

.... In an immunohistochemical study using collagen type-specific monoclonal antibodies, we asked whether type IX collagen, another ‘cartilage-specific’ collagen, is coexpressed along with type II at such interfaces. We confirmed that, in the matrix underlying a variety of cranial ectodermal derivatives and along the ventro...

...Keiji Itoh; Akiko Yamashita; Hiroshi Y. Kubota ABSTRACT Five kinds of monoclonal antibodies that are specific for the epidermis of Xenopus embryos were produced. Epidermis-specific antibodies were used to investigate the spatial and temporal expressions of epidermal antigens during embryonic...

...Michel Piovant; Philippe Léna We have generated a monoclonal antibody (Mab E1C) that recognizes the differentiated nervous system in Drosophila embryos. At the cellular blastoderm stage, Mab E1C behaves as a general ectodermal marker but, in subsequent stages, it also labels the mesoderm...

... in the wild-type strain WS380B; it is observed shortly after the advancing tip contacts the substratum. Stationary collars formed approximately every 12 min; they were matched with patterns revealed on the underside of slime trails with FITC-coupled monoclonal antibody MUD50. It is proposed that stationary...

.... Monoclonal antibodies were prepared against the isolated cytoplasm. Twelve monoclonal antibodies, identified by indirect immunofluorescence, stained the myoplasmic region. When unfertilized eggs were centrifuged, stratifying their mitochondria and some other cytoplasmic components, components identified...

...Izumi Mita-Miyazawa; Takahito Nishikata; Noriyuki Satoh ABSTRACT To obtain specific immunological probes for studying molecular mechanisms involved in the early embryonic development of ascidians, we have produced monoclonal antibodies directed against a homogenate of larvae of the ascidian...

... present address: Department of Biological Sciences, Columbia University, New York 10027, USA. Copyright © 1986 by Company of Biologists 1986 egg polarity oogenesis Xenopus laevis embryo yolk egg antigens yolk platelet compartments monoclonal antibodies cytoskeleton proteins...

...S. F. Godsave; B. H. Anderton; C. C. Wylie ABSTRACT Antibodies against various intermediate filament proteins have been used to follow cell differentiation in the early Xenopus embryo. Three new monoclonal antibodies against Xenopus cytokeratins raised against Triton-insoluble material from...

...Marian G. Langer; C. V. Sundarraj; Nirmala Sundarraj ABSTRACT Monoclonal antibodies, specific against cell surface differentiation antigens of human corneal epithelial cells, were developed using epithelial cells resected from human corneas as the immunogens. One of these antibodies reacted...

..., the molecule is present at a concentration of 2·5×10 3 molecules μ m -2 , which approximates HLA/ABC concentration on lymphocytes. By comparing the reactivity of four anti-XT-1 monoclonal antibodies, three of which elicit germ cell-germ cell adhesion, we have defined two distinct surface regions of the XT-l...

... fertilization. Indirect immunofluorescence studies with four monoclonal antibodies raised against carp spermatozoa revealed that monoclonal antibody WCS 29 stained the outer membranes of primordial germ cells in larvae from 3 days after fertilization. The monoclonal antibodies WCS 3 and 17 reacted...

...E. Lehtonen ABSTRACT Monoclonal antibodies were raised to detergent-extracted cytoskeleton preparations of mouse oocytes. In immunofluorescence microscopy, one of the antibodies, OCS-1, localizes exclusively to epithelial cells in frozen tissue sections, including various simple and stratified...

...Wai Chang Ho; Kathleen B. Bechtol ABSTRACT Four monoclonal antibodies, XT-I, MT-23, MT-24 and MT-29, that bind the XT-1-differentiation-antigen of male germ cells have been used to investigate the biological role of the XT-1-molecule of germ cells in short-term primary culture. Cultures from 10...

... are important topics of current research. The cytochemical approach with monoclonal antibodies in conjunction with glycosidases, as in the present study, provides a unique opportunity to visualize the in situ disposition of specific carbohydrate sequences in individual cells of the whole organism, and should...

... tissue. The regional binding patterns of a number of lectins and monoclonal antibodies specific for particular carbohydrate determinants are presented and their significance discussed. 1 Present address: Laboratory of Embryogenesis, National Institute for Medical Research, Mill Hill, London NW7...

... by some cultured human squamous cell carcinomas . Cell 25 , 627 – 635 . 10.1016/0092-8674(81)90170-7 Zanetti , M. RATCLIFFE , A. & Watt , F. M. ( 1985 ). Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulphate . J. Cell...