... Microinjection HRP In the mouse, gastrulation begins approximately midway through embryonic day (E) 6 (for reviews, see Tam and Behringer, 1997 ; Tam et al., 1993 ). At this time, the embryo consists of a single layer of primitive ectoderm (epiblast) covered by a layer of primitive endoderm...

... domain just C-terminal to the homeodomain, and a five amino acid GIRAW sequence at the C terminus ( Newman and Krieg, 1998 ). Homeobox genes Heart development Microinjection Xenopus laevis XNkx2-3 XNkx2-5 ...

... directly resulted in the elimination of precursor CNCC. Therefore, to determine whether Msx2 directs the apoptotic elimination of precursor CNCC in rhombomeres, chick embryos were microinjected with recombinant adenovirus and incubated in ovo for 12 hours until HH stage 10 – 11. Subsequently, individual...

... the normal developmental potential of neural crest cells by performing in situ cell lineage analysis in the mouse by microinjecting lysinated rhodamine dextran (LRD) into individual dorsal neural tube cells in the trunk. Labeled progeny derived from single cells were found in the neural tube, dorsal root...

... and the blastopore was located at the site of the future anus of the nauplius larva. In order to trace cell lineages and determine axial relationships, single 2and 4-cell-stage blastomeres were microinjected with rhodamine-dextran. The results showed that the mesendoderm cells which initiated gastrulation were...

... autofluorescence yolk microinjection Lucifer Yellow Rhodamine video enhancement During embryogenesis of the nematode C. elegans characteristic signs of tissue-specific differentiation develop. One prominent differentiation marker is a bluish autofluorescence under UV illumination, which appears...

... growth gastrulation microinjection HRP The epiblast or primitive ectoderm of the mouse embryo is the sole source of all the fetal tissues, both somatic and germline, and it also forms the amnion ectoderm and all the extraembryonic mesoderm ( Gardner and Papaionnou, 1975 ; Gardner and Rossant...

... of localization. Here, we test the developmental potential of migrating trunk neural crest cells by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells as they migrate through the somite. By two days after injection, the LRD-labelled clones contained from 2 to 67 cells, which were...

... State University, Terre Haute, Indiana 47809, USA © 1990 by Company of Biologists 1990 microinjection mosaicism RSV-LTR transgenes variegated expression zebrafish The production of transgenic organisms has been of great value in the study of the genetic basis of embry onic...

... transcriptional activation transgenic mice microinjection c-myc adenovirus chloramphenicol acetyltransferase β -galactosidase luciferase Differentiated cells (2.4 × 10 6 HL60 and 4.4 × 10 6 NIH/3T3) were washed twice in cold PBS by centrifuging for 5 min at 300g (4°C) and discarding...

... to populate their derivatives in a ventral-to-dorsal order, with the latest emigrating cells migrating exclusively along the dorsolateral pathway. © 1989 by Company of Biologists 1989 Dil microinjection pigment cells cell migration neural crest vital dye The neural crest is a population...

...Patrick H. Krone; John J. Heikkila ABSTRACT The expression of microinjected chimeric genes containing Drosophila hsp 70 and Xenopus hsp 70 and hsp 30 promoters linked to the reporter gene coding for bacterial chloramphenicol acetyltransferase (CAT) was examined during early development of Xenopus...

... segments. Such embryos can be ‘rescued’, i.e. restored to normal development, by microinjection of cytoplasm or RNA from unirradiated donor embryos. Most of the rescued embryos look completely normal and many of them hatch spontaneously. The rescuing activity decreases from the anterior to the posterior...

... to the metaphase. We show here that non-hormone-stimulated starfish oocytes, induced to mature by microinjection of MPF-containing cytosol taken from hormone-stimulated oocytes, produce normal bipin-naria larvae once fertilized. The same result was obtained when donor oocytes were induced to mature...

... be integrated into the fish genome, that the resulting transgenic fish are mosaic and that some of these mosaic individuals give rise to stable lines of transgenic fish. 23 02 1988 © 1988 by Company of Biologists 1988 germ-line transgenic zebrafish microinjection mosaicism DNA...

...Roberta R. Franks; Barbara R. Hough-Evans; Roy J. Britten; Eric H. Davidson A method is described for microinjection of cloned DNA into the zygote nucleus of Lytechinus variegatus . Eggs of this species are unusually transparent, facilitating visual monitoring of the injection process. The initial...

...Kirstie A. Lawson; Roger A. Pedersen; Sara Van De Geer ABSTRACT The fate of the embryonic endoderm (generally called visceral embryonic endoderm) of prestreak and early primitive streak stages of the mouse embryo was studied in vitro by microinjecting horseradish peroxidase into single axial...

... and microinjection of heterologous proteins using Hyalophora follicles. By microinjecting fluorescently labelled acidic and basic proteins into the nurse cells or oocyte of vitellogenic Drosophila follicles, we failed to obtain evidence for charge-dependent migration of these molecules. We have also analysed...

...Ulrich Scheer Copyright © 1986 by Company of Biologists 1986 nucleus amphibian oocyte microinjection antibodies lampbrush chromosomes transcription RNA polymerases chromatin actin. The chromatin constituents associated with transcriptionally active genes can be studied...

...D. A. Melton; M. R. Rebagliati Copyright © 1986 by Company of Biologists 1986 anti-sense RNA frog egg oocyte microinjection mRNA. In general, it is difficult to identify genes that play critical roles in developmental decisions in vertebrates because it is not possible...