...; David A. Prober; Matt van de Rijn; Jared R. Leadbetter; Dianne K. Newman; Carol Readhead; Marianne E. Bronner; Barbara Wold; Rusty Lansford; Tatjana Sauka-Spengler; Scott E. Fraser; Niles A. Pierce In situ hybridization methods are used across the biological sciences to map mRNA expression within intact...

... is disorganized in the Olig2-KO dorsal thalamus, and initial elongation of TCAs is retarded in the Olig2-KO forebrain. Microarray analysis demonstrated upregulation of several axon guidance molecules, including Epha3 and Epha5 , in the Olig2-KO basal forebrain. In situ hybridization showed that the prethalamus...

... ) * These authors contributed equally to this work 14 2 2005 ©2005. 2005 Planarians Regeneration Neoblasts Stem cells RNAi Cell proliferation Pumilio PUF proteins Confocal microscopy TEM Cytofluorimetry In situ hybridization Post-transcriptional regulation Stem cells...

... close to the floor plate, whereas LRN migrate and cross the floor plate ventro-marginally to locate contralaterally to their birthplace. We first delineated the cartography of Rho GTPases expression by in situ hybridization on hindbrain cryosections. ION were identified through their expression...

.... Evolutionary distances were calculated using Kimura’s equation ( Nei and Koehn, 1983 ), and used for phylogenetic tree construction by the Neighbor-joining method. Sequences were obtained from the EMBL/ GenBank. All sense and antisense digoxigenin-labeled RNA probes used for in situ hybridization...

... primitive streak. * Author for correspondence (e-mail: Schoenwolf@med.utah.edu ) 18 3 1999 4 5 1999 © 1999 by Company of Biologists 1999 Cardiac mesoderm Chick embryos Gastrulation Gene expression Heart In situ hybridization Neurulation Nieuwkoop center...

... . 10.1038/374360a0 New , D. A. T. ( 1955 ). A new technique for the cultivation of the chick embryo in vitro . J. Embryol. exp. Morph . 3 , 326 – 331 . Nieto , M. A. , Patel , K. and Wilkinson , D. G. ( 1996 ). In situ hybridization analysis of chick embryos in whole...

... repeats, DNA-binding domains, SH3-binding motifs and nuclear localization signals, are highly conserved. Northern blot analyses detect a single message of 8.9 and 5.8 kilo base (kb) from both cardiac and skeletal muscle of chick and mouse, respectively. In situ hybridization reveals that the cXin gene...

.... Fig. 8. Medium Acyl-CoA dehydrogenase (MCAD) mRNA expression in the mouse embryo. MCAD antisense riboprobe was used for in situ hybridization on: (A) mRNA is enriched in the day 13.5 embryonic heart; (B) MCAD mRNA expression in the wild-type ventricles and (C) in the heart of a RXRα mutant...

... Hensen’s node In situ hybridization Mesoderm Neurulation Nieuwkoop Center Regeneration Regulation The embryonic axis is progressively formed during vertebrate development through a series of inductive and suppressive cell-cell interactions and cellular movements. A striking example...

.../+ crosses. β-actin probe was provided by Janet Rossant. Both the Pax-3 and Pax-6 probes were the same as used for in situ hybridization. The assay was conducted as described by Ausubel et al. (1987) , using 10 µg of total cerebellar RNA and 5×105 cpm of probe RNA in each reaction. Quantitation...

.... Within the early gastrula Organizer, the genes Xbra, goosecoid, noggin and xNR3 are expressed differently in the animal-vegetal and superficial-deep dimensions. In situ hybridization and lineage labelling define distinct regions of the dorsal marginal zone. By the end of gastrulation, dorsal axial...

... somite defect neural tube defect in situ hybridization homologous recombination mouse RBP-Jк is a unique transcription factor that does not contain any of the known DNA-binding protein motifs such as zinc finger, helix turn helix, helix loop helix and leucine zipper ( Matsunami et al., 1989...

..., by northern analysis and in situ hybridization with 33 P-labelled antisense probes to each of the three mouse zona mRNAs, we have determined that the expression of each mouse zona gene is restricted to the oocyte. ZP2 transcripts, but not ZP1 or ZP3, are detected in resting (15 μm diameter) oocytes, and all...

... of the mammary secretory phenotype. By in situ hybridization, we have established evidence for asynchrony in milk protein gene expression among alveolar cells showing large variations in the intensity of hybridization among adjacent cells. The asynchrony of maturation of epithelial cells within a given alveolus...

... with the TGFα in situ hybridization, Dr Robert Tarrone for statistical analysis, and Dr Mirian Anver, Shirley Hale and Barbara Kasprzak for help with tissue preparation and histopathological analysis. The authors thank Greg Oblak for technical assistance. S. T. is grateful to the following for support: Medical...

...A. Feijen; M. J. Goumans; A. J. M. van den; Eijnden-van Raaij ABSTRACT Using in situ hybridization we have studied the localization of the messenger RNAs encoding the inhibin/activin subunits (α, βA, βB), the activin-binding protein follistatin and activin receptors (IIA, IIB) in mouse embryos...

... 19 06 1994 © 1994 by Company of Biologists 1994 GATA-2 Xenopus embryo activin noggin haematopoiesis ventral mesoderm animal cap in situ hybridization Despite the progress which has been made in understanding the control of haematopoiesis in adult animals...

... embryo fibroblast-conditioned medium, and stained tendons on frozen sections of chicken embryos. A cDNA probe specific for mRNA encoding repeat ‘C’ was used for in situ hybridization. This probe hybridized in a subset of the embryonic tissues labelled with a universal tenascin probe, including tendons...

... address: Department of Surgery, Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, MA 02115, USA 18 11 1993 © 1994 by Company of Biologists 1994 in situ hybridization promoter usage tissue-specific parental imprinting IGF2 H19 human development A subset...