..., strategically designed nested primers, optimised for speci-ficity, were used for amplification from the cDNA equiva-lent of a single embryo. The products were then verified by restriction enzyme digestion and sequence analysis. In the second method, immunocytochemistry has been used to co-localise the expressed...

... retinoic acid mouse embryogenesis immunocytochemistry Our observations indicating tissue-dependent downregulation of TGF β 1 and total down-regulation of TGF β 2 in all embryonic tissues are in contrast to previous observations from one of our laboratories in which it was clearly demonstrated...

... of a developmental process that involves generation of multiple cell types. ‪ Author for correspondence 13 10 1991 © 1992 by Company of Biologists 1992 6-hydroxydopamine differentiation immunocytochemistry BUdR goldfish retinal regeneration. The retina of teleost fish has...

...Christopher S. Von Bartheld; Susan L. Patterson; Josef G. Heuer; Esther F. Wheeler; Mark Bothwell; Edwin W. Rubel ABSTRACT The expression of nerve growth factor receptors (NGFRs) was studied in the developing inner ear with in situ hybridization in chick embryos and with immunocytochemistry in rat...

... is prepositioned in mesoderm-forming regions and is actively involved in mesoderm induction in vivo . * Corresponding author 26 06 1991 © 1991 by Company of Biologists 1991 fibroblast growth factor immunocytochemistry Xenopus mesoderm induction In Xenopus laevis blastulae...

... immunocytochemistry with antibodies against bromodeoxyuridine, BrdU (a thymidine analogue) and against opsin to determine when during the mitotic history of a cell the expression of opsin first occurred, taking a double labelled cell to be evidence of commitment to the rod cell fate. We found that the first double...

.... Although mature villous endocrine cells stained for DBA, immature endocrine cells did not, either in the positive crypts of chimaeric mouse gut or in gut from C57B1 positive controls. Thus a second marker was chosen. This experiment combined immunocytochemistry (to identify gastric antral gastrin cells...

... of Biologists 1990 IGF peptides immunocytochemistry chick embryo development The functions of growth factors in embryos are of major interest in developmental biology. Whilst there is a considerable body of information dealing with the effects of growth factors on cultured cells, relatively...

... © 1990 by Company of Biologists 1990 ontogeny immunocytochemistry process outgrowth pathway formation axonal growth pattern propriospinal spinal cord avian Previous studies, including two reports from our labora-tory, have shown that intersegmental projection neur-ons...

... polyclonal antiserum to desmosomal glycoproteins 2 and 3 (dg 2&3) (desmocollins) has been described and characterised ( Cowin and Garrod, 1983 ). For immunocytochemistry, kidneys were snap frozen in liquid nitrogen and sectioned at 4 μm on a cryostat and some human kidneys were fixed in 10...

... . * Author for correspondence © 1989 by Company of Biologists 1989 keratan sulfate chondroitin sulfate immunocytochemistry limb development Keratan sulfate is a glycosaminoglycan that has been identified as a matrix component of two types of connective tissues, corneal stroma...

... reflect aspects of the divergent differen-tiational pathways of the two follicle cell types. 29 04 1987 © 1987 by Company of Biologists 1987 differentiation immunocytochemistry yolk polypeptides Sarcophaga bullata Diptera ovary follicle cells Meroistic polytrophic egg...

...: Department of Histopathology, St George’s Hospital Medical School, Tooting, London, UK. 04 08 1986 Copyright © 1986 by Company of Biologists 1986 mouse embryo neural crest cells type-IV collagen basement membranes immunocytochemistry Trunk neural crest cells (NCCs...

..., and should facilitate systematic investigations of their functions. 16 07 1985 Copyright © 1985 by Company of Biologists 1985 carbohydrate structures carbohydrate antigens embryonic antigens differentiation antigens mouse embryo saccharides immunocytochemistry monoclonal antibodies...