...Laura J. Harrison; Daniel Bose ABSTRACT Enhancers confer precise spatiotemporal patterns of gene expression in response to developmental and environmental stimuli. Over the last decade, the transcription of enhancer RNAs (eRNAs) – nascent RNAs transcribed from active enhancers – has emerged...

... expression remains unclear. Depletion of molecular factors (e.g. CTCF) underlying topologically associating domains (TADs) leads to modest alterations in gene expression, whereas genomic rearrangements involving TAD boundaries disrupt normal gene expression and can lead to pathological phenotypes. Here, we...

... the sexes. Sex difference Diet Insulin Gene expression Metabolism Body size Drosophila Canadian Institutes of Health Research http://dx.doi.org/10.13039/501100000024 PJT-153072 GS4-171365 Natural Sciences and Engineering Research Council of Canada http://dx.doi.org...

... cells rapidly diversify from each other, and investigation of the corresponding gene expression dynamics can help elucidate underlying cellular differentiation programs. However, current plant embryonic transcriptome datasets either lack cell-specific information or have RNA contamination from...

... and concomitantly undergo stronger downregulation by knockdown of either the intracellular Ca 2+ release channel IP 3 R or Set2. IP 3 /Ca 2+ signals thus regulate gene expression through Set2-mediated H3K36 marks on select neuronal genes for the larval to pupal transition. We thank the Central Imaging and Flow...

...Ian J. Begeman; Kwangdeok Shin; Daniel Osorio-Méndez; Andrew Kurth; Nutishia Lee; Trevor J. Chamberlain; Francisco J. Pelegri; Junsu Kang ABSTRACT Heart regeneration in regeneration-competent organisms can be accomplished through the remodeling of gene expression in response to cardiac injury...

... to also play a role in promoting TA. Some long non-coding RNAs (lncRNAs) are known to activate gene expression by directly binding to the COMPASS (complex proteins associated with Set1) complex and increasing H3K4me3 marks on target gene promoters ( Khalil et al., 2009 ). Accordingly, it was reported...

... maturation are unknown. The transition from the developmental to maturation phase is accompanied by a transcriptional switch in the expression of ∼700 genes that occurs from E12 to E13.5 ( Knox and Baker, 2008 ). These dramatic gene expression changes imply a role for global transcriptional regulators...

...Anna Reade; Laura B. Motta-Mena; Kevin H. Gardner; Didier Y. Stainier; Orion D. Weiner; Stephanie Woo ABSTRACT Here, we describe an optogenetic gene expression system optimized for use in zebrafish. This system overcomes the limitations of current inducible expression systems by enabling robust...

...Swetansu K. Hota; Benoit G. Bruneau Precise gene expression ensures proper stem and progenitor cell differentiation, lineage commitment and organogenesis during mammalian development. ATP-dependent chromatin-remodeling complexes utilize the energy from ATP hydrolysis to reorganize chromatin...

... of genes along the apical-basal and dorsal-ventral axes in the globular embryo, which tended to fluctuate in later developmental stages. We also analyzed gene expression patterns in the early globular embryo and how this relates to expression in embryonic organs at later stages. We confirmed the accuracy...

...L. Carine Stapel; Benoit Lombardot; Coleman Broaddus; Dagmar Kainmueller; Florian Jug; Eugene W. Myers; Nadine L. Vastenhouw Analysis of differential gene expression is crucial for the study of cell fate and behavior during embryonic development. However, automated methods for the sensitive...

... and Newcastle, UK, was established to provide embryonic and fetal material for a variety of human studies ranging from single gene expression analysis to large-scale genomic/transcriptomic studies. Increasingly, HDBR material is enabling the derivation of stem cell lines and contributing towards developments...

... gene expression during mesoderm induction. We find that Brg1 co-localizes with H3K27ac at distal enhancers and is required for robust H3K27 acetylation at distal enhancers that are activated during mesoderm induction. Brg1 is also required to maintain Polycomb-mediated repression of non-mesodermal...

...Ahmet Ay; Jack Holland; Adriana Sperlea; Gnanapackiam Sheela Devakanmalai; Stephan Knierer; Sebastian Sangervasi; Angel Stevenson; Ertuğrul M. Özbudak The vertebrate segmentation clock is a gene expression oscillator controlling rhythmic segmentation of the vertebral column during embryonic...

... permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Pancreas development Endocrine progenitor cells Gene expression Transcription factors Mouse The genetic program responsible for the generation of pancreatic...

... the atlas-builder software to benefit a large proportion of the developmental biology community, and the eNeuro atlas to serve as a publicly accessible hub for integrating neuronal attributes – cell lineage, gene expression patterns, axon/dendrite projections, neurotransmitters – and linking them...

... division Gene expression RNA FISH Time-lapse Timing Transcription The process of animal embryonic development begins at fertilization and the creation of a single cell zygote. From there, this cell will divide into many more cells, and these cells will eventually acquire different...

...Benedetta Artegiani; Federico Calegari Generation of transgenic mice, in utero electroporation and viral injection are common approaches to manipulate gene expression during embryonic development of the mammalian brain. While very powerful in many contexts, these approaches are each characterized...

... statement The authors declare no competing financial interests. 6 8 2012 © 2012. 2012 Cohesin Polycomb Gene expression y wapl AG w UAS-Wapl-S/FM0 virgin females were crossed to arm-Gal4 homozygous males. Progeny were 14 males of the genotype y wapl AG w UAS-Wapl-S...