...Yuu Usui; Toshihiro Aramaki; Shigeru Kondo; Masakatsu Watanabe ABSTRACT Connexin 39.4 (Cx39.4) and connexin 41.8 (Cx41.8), two gap-junction proteins expressed in both melanophores and xanthophores, are crucial for the intercellular communication among pigment cells that is necessary for generating...

... of LR asymmetric genes in the left LPM. In Sox17 mutant endoderm, the localization of connexin proteins on the cell membrane was greatly reduced, resulting in defective gap junction formation, which appeared to be caused by incomplete development of organized epithelial structures. Our findings suggest...

...(n585) allele used for this experiment ( Fig. 3F ). Experiments with the null allele egl-20(hu120) confirmed that EGL-20 is necessary for the mis-wiring of posterior VAs with gap junctions from AVB in unc-4 but also suggest that a minimum level of EGL-20 activity might be required to promote...

...Chun-hong Xia; Haiquan Liu; Debra Cheung; Catherine Cheng; Eddie Wang; Xin Du; Bruce Beutler; Woo-Kuen Lo; Xiaohua Gong Different mutations of α3 connexin (Cx46 or Gja8 ) andα8 connexin (Cx50 or Gja8 ), subunits of lens gap junction channels, cause a variety of cataracts via unknown mechanisms. We...

...Lilach Gilboa; Alexandria Forbes; Salli I. Tazuke; Margaret T. Fuller; Ruth Lehmann Gap junctions coordinate processes ranging from muscle contraction to ovarian follicle development. Here we show that the gap junction protein Zero population growth (Zpg) is required for germ cell differentiation...

... slices, ESGPs actively migrate into the recipient tissue and establish extensive cell-cell contacts with recipient glia. Gap junction-mediated coupling between donor and host astrocytes permits widespread delivery of dye from single donor cells. During maturation,engrafted donor cells display...

... population growth ( zpg ) locus of Drosophila encodes a germline-specific gap junction protein, Innexin 4, that is required for survival of differentiating early germ cells during gametogenesis in both sexes. Animals with a null mutation in zpg are viable but sterile and have tiny gonads. Adult zpg -null...

... of its cell body and its subsequent intracellular staining with Lucifer Yellow. These preparations provide high-resolution data on motorneuron morphogenesis during the first half of pupal development. Dye-coupling reveals onset of gap-junction formation between the tergotrochanteral motorneuron and other...

... are consistent with our previously proposed model in which the orientation of LR asymmetry in the frog, Xenopus laevis , depends on large-scale partitioning of LR determinants through intercellular gap junction channels (M. Levin and M. Mercola (1998) Developmental Biology 203, 90-105). Here we evaluate whether...

...J. L. Ewart; M. F. Cohen; R. A. Meyer; G. Y. Huang; A. Wessels; R. G. Gourdie; A. J. Chin; S. M. J. Park; B. O. Lazatin; S. Villabon; C. W. Lo ABSTRACT Transgenic mice were generated containing a cytomegaloviral promoter driven construct (CMV43) expressing the gap junction polylpeptide connexin 43...

...D. L. Paul; K. Yu; R. Bruzzone; R. L. Gimlich; D. A. Goodenough ABSTRACT A chimeric construct, termed 3243H7, composed of fused portions of the rat gap junction proteins connexin32 (Cx32) and connexin43 (Cx43) has been shown to have selective dominant inhibitory activity when tested in the Xenopus...

...Florenci Serras; Scott Fraser; Cheng-Ming Chuong ABSTRACT To study the pattern of gap junctional communication in chicken skin and feather development, we injected Lucifer Yellow into single cells and monitored the transfer of the fluorescent dye through gap junctions. Dye coupling is present...

...David L. Becker; Catherine Leclerc-David; Anne Warner In the mouse embryo, gap junctions first appear at the 8-cell stage as compaction is about to take place. Compaction of the embryo is important for the differentiation of the first two cell types; the inner cell mass and the trophectoderm. Our...

...D. J. Nagajski; S. C. Guthrie; C. C. Ford; A. E. Warner ABSTRACT The correlation between cell-to-cell communication through gap junctions at the 32-cell stage and the subsequent patterning of the embryonic axis has been examined in Xenopus laevis embryos. Disturbances of embryonic axis formation...

...Sarah Guthrie; Luca Turin; Anne Warner Cell-cell communication through gap junctions was examined in Xenopus laevis embryos between the 16cell and early blastula stages using Lucifer Yellow, Fluorescein, lead EDTA and dicyanoargentate as probes of junctional permeability. Injections were made...

...Mia Buehr; S. Lee; Anne Mclaren; Anne Warner ABSTRACT Communication through gap junctions was examined in 8-cell zygotes generated by fertilization of eggs of the DDK inbred strain of mice with spermatozoa of the C3H strain. These zygotes spontaneously begin to extrude cells at the late 16-cell...