... Theiler stage 12-13 mouse embryos to identify 8921 putative notochord enhancers. In addition, we established a new model for generating notochord-like cells in culture, and found 3728 of these enhancers occupied by the essential notochord control factors brachyury (T) and/or Foxa2. We describe...

... their development. Primordial germ cell Gastruloid Embryo Stem cell Cell interactions Francis Crick Institute http://dx.doi.org/10.13039/100010438 Cancer Research UK http://dx.doi.org/10.13039/501100000289 CC2186 Medical Research Council http://dx.doi.org/10.13039...

...Peter J. Rugg-Gunn; Naomi Moris; Patrick P. L. Tam ABSTRACT Recent years have seen exciting progress across human embryo research, including new methods for culturing embryos, transcriptional profiling of embryogenesis and gastrulation, mapping lineage trajectories, and experimenting on stem cell...

... microtubules. Recruitment of SAC proteins to unattached kinetochores generates an inhibitory signal that prolongs mitotic duration. Chordate embryos are atypical in that spindle defects do not delay mitotic progression during early development, implying that either the SAC is inactive or the cell-cycle target...

... (IL1) is an important regulator of aorta HSC development by limiting the differentiation of HSCs along the myeloid lineage ( Orelio et al., 2008 ). Il1 and its receptor Il1r1 are expressed at the mid-gestation stage of the mouse embryo, around the AGM, with Il1r being concentrated at the ventral...

... at the presomitic to the 3-somite stage in the avian embryo . Development 103 , 101 - 113 . 10.1242/dev.103.Supplement.101 Dhouailly , D. ( 1973 ). Dermo-epidermal interactions between birds and mammals: differentiation of cutaneous appendages . J. Embryol. Exp. Morphol. 30 , 587 - 603...

... moellendorffii (SmBRX) in an A. thaliana wild-type background confers robustly enhanced root growth vigor that persists throughout the life cycle. This effect can be traced to a substantial increase in seed and embryo size, is associated with enhanced vascular tissue proliferation, and can be reproduced...

... by The Company of Biologists Ltd 2022 Summary: This Review presents the intrinsic and extrinsic mechanical forces acting on the embryonic gut and their determining role in structuring the development of this organ. Biomechanics Intestine Embryo Mouse Chicken Human Université de Paris...

... of the mechanistic underpinnings of neuroblastoma. Embryo Epithelial-to-mesenchymal transition Neural crest Neuroblastoma European Social Fund http://dx.doi.org/10.13039/501100004895 MIS 5033021 State Scholarships Foundation http://dx.doi.org/10.13039/501100003447...

... , J. C. and Van Wyk , J. H. ( 2021 ). Lethal and teratogenic impacts of imazapyr, diquat dibromide, and glufosinate ammonium herbicide formulations using frog embryo teratogenesis assay- Xenopus (FETAX) . Arch. Environ. Contam. Toxicol. 80 , 708 - 716 . 10.1007/s00244-020-00756-5...

... reprogramming contributes significantly to the dynamic gene expression during ZGA; however, the mechanism has yet to be resolved. Here, we find histone deacetylases 1 and 2 (HDAC1/2) can regulate ZGA through lysine deacetylase activity. Notably, in mouse embryos, overexpression of a HDAC1/2 dominant-negative...

...Ming-Zhe Dong; Ying-Chun Ouyang; Shi-Cai Gao; Xue-Shan Ma; Yi Hou; Heide Schatten; Zhen-Bo Wang; Qing-Yuan Sun ABSTRACT Mammalian early embryo cells have complex DNA repair mechanisms to maintain genomic integrity, and homologous recombination (HR) plays the main role in response to double-strand...

... reproduction. Despite our knowledge of mitotic mis-segregation in somatic cells, the molecular pathways regulating chromosome fidelity during the error-prone cleavage-stage of mammalian embryogenesis remain largely undefined. Using bovine embryos and live-cell fluorescent imaging, we observed frequent micro...

... to implantation. Unfortunately, our understanding of these key processes for embryo survival is limited because current culture systems cannot sustain ungulate embryo development beyond hatching . Here, we report a culture system that recapitulates most developmental landmarks of gastrulating ovine embryos...

... and transcriptome analyses of two-cell embryos revealed that, although ZGA took place, there were subtle defects in embryos that lacked maternal Dppa2/4. Strikingly, heterozygous offspring that inherited the null allele maternally had higher preweaning lethality than those that inherited the null allele paternally...

... is the prime organizing center for axial elongation in mouse embryos. The T-box transcription factor brachyury (T) is essential for both formation of the notochord and maintenance of NMPs, and thus is a key regulator of trunk and tail development. The T promoter controlling T expression in NMPs and nascent...

... surrounding non-embryonic tissues . We have coupled fluorescence-activated nuclei sorting together with single-nucleus mRNA-sequencing to construct a gene expression atlas of Arabidopsis thaliana early embryos at single-cell resolution. In addition to characterizing cell-specific transcriptomes, we found...

... determination occurs after PGC colonization of the gonads, and that germ cell tumors reflect a failure of this process. Determination Embryo Germ cell tumor Germline Potency Primordial germ cell The germline is the cell lineage, from zygote to gamete, with the capacity or potential...

...-computed tomography (micro-CT), have been incorporated into the high-throughput embryo pipeline of the International Mouse Phenotyping Consortium (IMPC). This project generates large volumes of raw data that cannot be immediately exploited without significant resources of personnel and expertise. Thus...

... marks a stage of developmental divergence between key model organisms, and thus potential variance in the cell and molecular mechanisms for PGC specification. In humans, PGC specification is very difficult to study in vivo . To address this, the combined use of human and nonhuman primate embryos...