...Alaa Hachem; Jonathan Godwin; Margarida Ruas; Hoi Chang Lee; Minerva Ferrer Buitrago; Goli Ardestani; Andrew Bassett; Sebastian Fox; Felipe Navarrete; Petra de Sutter; Björn Heindryckx; Rafael Fissore; John Parrington ABSTRACT Activation of the egg by the sperm is the first, vital stage...

... for single-frequency CARS microscopy of LDs. Living mouse oocytes, eggs and early embryos were imaged label-free using differential interference contrast (DIC) and CARS microscopy. Fig. 1 A-G shows DIC images in a single, approximately equatorial z -plane of all developmental stages, from germinal vesicle...

... and other viscera. In the radially symmetric egg of Xenopus laevis , the earliest steps in DV axis determination are driven by microtubule-dependent localization of maternal components toward the prospective dorsal side. LR axis determination is linked in time to this DV-determining process...

...Rémi Dumollard; Petros Marangos; Greg Fitzharris; Karl Swann; Michael Duchen; John Carroll At fertilisation, repetitive increases in the intracellular Ca 2+ concentration, [Ca 2+ ] i , drive the completion of meiosis and initiate the development of the quiescent egg into an embryo. Although...

...Karolina Piotrowska; Magdalena Zernicka-Goetz The first cleavage of the fertilised mouse egg divides the zygote into two cells that have a tendency to follow distinguishable fates. One divides first and contributes its progeny predominantly to the embryonic part of the blastocyst, while the other...

...Xudong Shi; Simin Amindari; Kavitha Paruchuru; Dale Skalla; Heather Burkin; Barry D. Shur; David J. Miller ABSTRACT ZP3 is a protein in the mammalian egg coat (zona pellucida) that binds sperm and stimulates acrosomal exocytosis, enabling sperm to penetrate the zona pellucida. The nature of the ZP3...

... normal cleavage to the 8-cell stage. We discuss that although polarity of the postimplantation embryo can be traced back to the 8-cell stage and in turn to the organisation of the egg, it is not absolutely fixed by this time. The polar bodies from the meiotic divisions define the animal pole of the egg...

...Tie-Shan Tang; Jian-Bo Dong; Xiu-Ying Huang; Fang-Zhen Sun ABSTRACT At fertilization in mammals, the sperm activates the egg by inducing a series of oscillations in the intracellular free Ca 2+ concentration. There is evidence showing that this oscillatory event is triggered by a sperm-derived...

...Fabrice Roegiers; Chakib Djediat; Ré Dumollard; Christian Rouviè; Christian Sardet ABSTRACT Many eggs undergo reorganizations that localize determinants specifying the developmental axes and the differentiation of various cell types. In ascidians, fertilization triggers spectacular reorganizations...

...Joanne Shearer; Céline De Nadai; Françoise Emily-Fenouil; Christian Gache; Michael Whitaker; Brigitte Ciapa ABSTRACT It is well known that stimulation of egg metabolism after fertilization is due to a rise in intracellular free calcium concentration. In sea urchin eggs, this first calcium signal...

...Keith T. Jones; Christian Soeller; Mark B. Cannell SUMMARY Mouse sperm-egg fusion was examined using two-photon and confocal microscopy. A delay of several minutes occurred between the first observable event of fusion (which was the diffusion of Ca 2+ -sensitive dyes from egg into sperm) and any...

...Evelyn Houliston ABSTRACT The development of dorsal axial structures in frogs depends on a process of cortical rotation in which the cortex of the fertilised egg becomes displaced with respect to the cytoplasm. An array of aligned microtubules that develops between the vegetal cortex and cytoplasm...

... egg embryo plasma membrane oolemma fertilization Fertilization begins with membrane fusion between the spermatozoon and oocyte and ends with the mingling of the male and female genome. Although the morphology of sperm-oocyte membrane fusion in mammals has been studied extensively using...

...J. M. W. Slack; H. V. Isaacs Mesoderm-inducing activity can be extracted from Xenopus embryos, eggs or whole ovary. It binds to heparin and can be neutralized by heparin or anti-bFGF but not by anti-TGF β . Two molecular forms can be identified by Western blotting and have molecular weights...

...C. Aimar ABSTRACT The role of the cytoplasm and nucleus in the control of the length of the division cycle was investigated in Pleurodeles waltl eggs. Injection of spermine into enucleated eggs showed that the ability to cleave was not restricted just to the period of normal cytokinesis (T = 1·0...

...Sabera Ruzdijic; Thoru Pederson ABSTRACT Psoralen crosslinking of RNA–RNA intermolecular duplexes in sea urchin egg extracts reveals that some maternal poly(A) + RNA molecules are complexed with U1 RNA, a cofactor in somatic nuclear pre-mRNA splicing. Reaction of egg extracts with a monoclonal...

...Klaus Kalthoff; Ahmed Elbetieha Copyright © 1986 by Company of Biologists 1986 Chironomus microinjection egg determinants anterior transplantation. In most animal embryos early determinative steps appear to be controlled by uneven distributions of cytoplasmic components...