...Timothy J. Duerr; Eun Kyung Jeon; Kaylee M. Wells; Antonio Villanueva; Ashley W. Seifert; Catherine D. McCusker; James R. Monaghan ABSTRACT Regulation of cell cycle progression is essential for cell proliferation during regeneration following injury. After appendage amputation, the axolotl...

...Sarah E. Walker; Keith Z. Sabin; Micah D. Gearhart; Kenta Yamamoto; Karen Echeverri ABSTRACT Axolotls are an important model organism for multiple types of regeneration, including functional spinal cord regeneration. Remarkably, axolotls can repair their spinal cord after a small lesion injury...

... BMP signaling is blocked, but ectopic injection of either human or axolotl protein did not rescue the defects. By administering LDN193189 treatments at different time points during regeneration, we show clearly that limb regeneration progresses in a proximal to distal fashion. This demonstrates...

... an overview of salamanders as a model system for studying regeneration, outlining the mechanisms deployed during salamander regeneration and highlighting how the study of salamanders has increased our understanding of regeneration more broadly. CNS Axolotl Genome Limb Model organism Newt...

..., axolotl and Xenopus laevis , in as little as 1-5 days, while preserving a broad range of fluorescent proteins, including GFP, mCherry, Brainbow and Alexa-conjugated fluorophores. Ethyl cinnamate is non-toxic and can easily be used in multi-user microscope facilities. This method opens up tissue clearing...

... blastemal progenitors remain unknown. We transcriptionally profiled dividing and non-dividing cells in regenerating stump tissues, as well as the wound epidermis, during early axolotl limb regeneration. Our analysis revealed unique transcriptional signatures of early dividing cells and, unexpectedly...

.... Procedures for care and manipulation of all animals used in this study were performed in compliance with the Animals (Scientific Procedures) Act 1986, approved by the United Kingdom Home Office. Axolotl eggs ( Ambystoma mexicanum ) were obtained from Neil Hardy Aquatica (Croydon, UK) and maintained...

...Jean-François Denis; Fadi Sader; Samuel Gatien; Éric Villiard; Anie Philip; Stéphane Roy Axolotls are unique among vertebrates in their ability to regenerate tissues, such as limbs, tail and skin. The axolotl limb is the most studied regenerating structure. The process is well characterized...

...Johanna E. Farkas; Polina D. Freitas; Donald M. Bryant; Jessica L. Whited; James R. Monaghan The Mexican axolotl ( Ambystoma mexicanum ) is capable of fully regenerating amputated limbs, but denervation of the limb inhibits the formation of the post-injury proliferative mass called the blastema...

... Opossum Turtle Axolotl Xenopus Increased heterogeneity in neural progenitor pools and the precise regulation of progenitor dynamics are essential for the evolution of intricate brain architectures. In the developing mammalian neocortex, two types of neuronal progenitors are distinguished...

... to identify the ancestral mechanism of PGC specification in vertebrates, we studied PGC specification in embryos from the axolotl (Mexican salamander), a model for the tetrapod ancestor. In the axolotl, PGCs develop within mesoderm, and classic studies have reported their induction from primitive ectoderm...

...G. Parker Flowers; Andrew T. Timberlake; Kaitlin C. Mclean; James R. Monaghan; Craig M. Crews Among tetrapods, only urodele salamanders, such as the axolotl Ambystoma mexicanum , can completely regenerate limbs as adults. The mystery of why salamanders, but not other animals, possess this ability...

...Jessica L. Whited; Stephanie L. Tsai; Kevin T. Beier; Jourdan N. White; Nadine Piekarski; James Hanken; Constance L. Cepko; Clifford J. Tabin Axolotls are poised to become the premiere model system for studying vertebrate appendage regeneration. However, very few molecular tools exist for studying...

... is dependent on the expression of Nanog . Pluripotency is conserved in the primitive ectoderm of embryos from mammals and urodele amphibians, and here we report the isolation of a Nanog ortholog from axolotls ( axNanog ). axNanog does not contain a tryptophan repeat domain and is expressed as a monomer...

...Levan Mchedlishvili; Hans H. Epperlein; Anja Telzerow; Elly M. Tanaka Complete regeneration of the spinal cord occurs after tail regeneration in urodele amphibians such as the axolotl. Little is known about how neural progenitor cells are recruited from the mature tail, how they populate...

... of proximal cell identity during limb development. To understand the molecular basis for specifying proximal positional identities during regeneration, we isolated the axolotl Meis homeobox family. Axolotl Meis genes are RA-regulated during both regeneration and embryonic limb development. During limb...

... determines the DV patterning of the regenerating spinal cord as well as the patterning of surrounding tissues such as cartilage. We investigated this phenomenon on a molecular level. Both the mature and regenerating axolotl spinal cord express molecular markers of DV progenitor cell domains found during...

...Andrea R. Morris; Julie Drawbridge; Malcolm S. Steinberg The epidermis overlying the migrating axolotl pronephric duct is known to participate in duct guidance. This epidermis deposits an extracellular matrix onto the migrating duct and its pathway that is a potential source of directional guidance...

.... To test this idea, tissue contacts during gastrulation were manipulated systematically in axolotl embryos, and the subsequent ability of the pharyngeal endoderm to generate taste buds was assessed. Disruption of both putative planar and vertical signals from neurectoderm failed to prevent...

...Hans-Henning Epperlein; Daniel Meulemans; Marianne Bronner-Fraser; Herbert Steinbeisser; Mark A. J. Selleck ABSTRACT We have examined the ability of normal and heterotopically transplanted neural crest cells to migrate along cranial neural crest pathways in the axolotl using focal DiI injections...