.../licenses/by/4.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Highlighted Article: Using genomic engineering in mouse embryos and embryonic stem cells, expression of Xist , which regulates X-inactivation...

...Yuko Hoki; Rieko Ikeda; Nathan Mise; Yuka Sakata; Tatsuya Ohhata; Hiroyuki Sasaki; Kuniya Abe; Takashi Sado X chromosome inactivation (X-inactivation) in female mammals is triggered by differential upregulation of the Xist gene on one of the two X chromosomes and subsequent coating of the X in cis...

...Yuko Hoki; Naomi Kimura; Minako Kanbayashi; Yuko Amakawa; Tatsuya Ohhata; Hiroyuki Sasaki; Takashi Sado X-inactivation in female mammals is triggered by the association of non-coding Xist RNA in cis with the X chromosome. Although it has been suggested that the A-repeat located in the proximal part...

...Takashi Sado; Yuko Hoki; Hiroyuki Sasaki Dosage differences of X-linked genes between male and female mammals are compensated for by a mechanism known as X-inactivation, and the noncoding Xist gene plays a crucial role in this process. The expression of Xist is regulated in cis by its noncoding...

... of the epiblast or trophoblast. Chimeras generated by injection of XEN cells into blastocysts showed exclusive contribution to extra-embryonic endoderm cell types. We used female XEN cells to investigate the mechanism of X chromosome inactivation in this lineage. We observed paternally imprinted X-inactivation...

... transiently to one X chromosome in female embryos at the onset of X-inactivation. This accumulation and the associated histone methylation are also lost in Ezh2 mutants, suggesting a role in X inactivation. Thus,Ezh2 has significant and diverse roles during early development, as well as during...

... is reminiscent of asymmetries observed for the human X chromosome in the female soma. The arm of the X chromosome which contains the X-inactivation center, Xq, is enriched for interspersed repeated LINE-1 elements which themselves are enriched in α-heterochromatin ( Bailey et al., 2000 ). Conversely, genes...

..., in regulation of Xist expression and X- inactivation. We show that Tsix is transcribed from two putative promoters and its transcripts are processed. Expression of Tsix is first detected in blastocysts and is imprinted with only the maternal allele transcribed. The imprinted expression of Tsix persists...

...@cvrc.mgh.harvard.edu ) 09 07 1996 © 1996 by Company of Biologists 1996 DNA cytosine methyltransferase de novo methylation genomic imprinting X-inactivation gene targeting embryonic stem cells Methylation of DNA at the C5 position of cytosine residues plays an important role...

..., suggest a purpose for genomic imprinting, and postulate a general means for regulating the time in development at which certain genes become heterochromatically repressed. Copyright © 1990 by Company of Biologists 1990 Drosophila position effect variegation X-inactivation chromosome...

... with the 12E7 antigen. These molecular tools have been used to investigate the genetics and biochemistry of the MIC2 system. Recent results suggest that MIC2 is the most proximal of the currently defined pseudoautosomal markers and that the escape of M1C2 from X-inactivation may be intrinsic to an associated...

... is discussed. * Present address: Murdoch Institute for Research into Birth Defects, Royal Children’s Hospital, Flemington Road, Parkville, Victoria 3052 Australia 24 11 1986 © 1987 by Company of Biologists 1987 gynogenetic parthenogenetic X-inactivation X chromosome imprinting...

... derived X chromosome are preferentially inactivated in the extraembryonic endoderm we investigated whether a line of transgenic mice (47–3–13) bearing an X-linked AFP gene would also exhibit X-inactivation ( Krumlauf et al . 1986 ). In this line of mice the X-linked AFP gene was expressed on both...

... either reversal of inactivation, or the frequency with which the influence of the X chromosome extends to the albino locus. 11 04 1986 Copyright © 1986 by Company of Biologists 1986 X-inactivation mosaics pigmentation genetic activity Cattanach’s insertion albino mouse mutation...