... embryos. Mechanistically, Csde1 regulates HSPC generation through modulating Wnt/β-catenin signaling activity. We demonstrate that Csde1 binds to ctnnb1 mRNAs (encoding β-catenin, an effector of Wnt signaling) and regulates translation but not stability of ctnnb1 mRNA, which further enhances β-catenin...

... ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: Commissural axons leave the floor plate and turn rostral by a smooth transition from Robo to Wnt signaling. Cables1 links these pathways by transferring...

... canonical Wnt signaling intracellularly in larval wings, and that it activates canonical Wnt signaling as well as Fz/PCP at pupal stages ( Banerjee et al., 2023 ). Our data support the proposed Wnt antagonist role. However, we also found that Fz4 lost its KTxxxW domain in Diptera and Lepidoptera ( Fig. S1...

.... , Diecke , S. , Huber , B. , Mordwinkin , N. M. et al. ( 2014 ). Chemically defined generation of human cardiomyocytes . Nat. Methods 11 , 855 - 860 . 10.1038/nmeth.2999 Cadigan , K. M. and Waterman , M. L. ( 2012 ). TCF/LEFs and Wnt signaling in the nucleus...

... of canonical Wnt signaling, accumulated. We identified Wnt7b as a factor with increased expression in growth plate cartilage in Smad4 cKO mice. Wnt7b mRNA was expressed in differentiated chondrocytes and suppressed by BMP4 stimulation. Ablation of Wnt7b blunted the increase in bone in adult Smad4 cKO mice...

... control of cell subpopulations can be used to uncover signaling feedback mechanisms between neighboring cell types. These findings reveal that cell-to-cell variability in Wnt signaling is sufficient to generate tissue-scale patterning and establish a hESC model system for investigating feedback mechanisms...

...Pavel Tsaytler; Jinhua Liu; Gaby Blaess; Dennis Schifferl; Jesse V. Veenvliet; Lars Wittler; Bernd Timmermann; Bernhard G. Herrmann; Frederic Koch ABSTRACT Cardiac lineage specification in the mouse is controlled by TGFβ and WNT signaling. From fly to fish, BMP has been identified...

... canonical Wnt signaling as a key signaling pathway primarily active in cranial neural crest (CNC)-derived mesenchymal cells surrounding soft palatal myogenic cells. Using Osr2-Cre;β-catenin fl/fl mice, we show that Wnt signaling is indispensable for mesenchymal cell proliferation and subsequently...

... a highly penetrant cleft palate phenotype, shortened limbs compared with wild type and perinatal lethality. Fzd2 D4 craniofacial tissues indicated decreased canonical Wnt signaling. In utero treatment with IIIC3a (a DKK inhibitor) normalized the limb lengths in Fzd2 D4 homozygotes. The in vivo replication...

... (WREs), to activate target gene transcription. Discovering the binding profile of β-catenin is therefore required to unambiguously assign direct targets of WNT signaling. Cleavage under targets and release using nuclease (CUT&RUN) has emerged as prime technique for mapping the binding profile of DNA...

... deletion showed that melanocytes and immune cells dynamically follow epithelial scale:interscale patterns. Importantly, disrupting Wnt-Lef1 function in keratinocytes caused melanocyte mislocalization to interscale epidermis, implicating canonical Wnt signaling in organizing the pigmentation pattern...

... be addressed with in vitro models of human embryos that are derived from embryonic stem cells (hESCs). Our previous work demonstrated that during early stages of hESC differentiation, cells chronicle signaling hierarchy. Only cells that have been exposed (primed) by WNT signaling can respond to subsequent...

... and barrier development, also functions as an integral unit in limb development. Wnt signaling Blood-brain barrier Limb development Mouse Howard Hughes Medical Institute Johns Hopkins University The vertebrate limb has been one of the premier systems...

... pathway have shown that tooth formation is partly inhibited in loss-of-function mutants, and gain-of-function mutants have perturbed tooth morphology. However, the stage at which Wnt signaling is first important in tooth formation remains unclear. Here, using an Fgf8 -promoter-driven, and therefore early...

... that have been conserved over hundreds of millions of years of evolution. These enhancer elements also each contain a conserved perfect consensus-binding site for Tcf7/Lef1, which are the Wnt-regulated transcription factors in cells, revealing how Hox factors and Wnt signaling could intersect to promote...

... for reproduction, but how this is achieved is unclear. Here, we show that canonical wingless-type MMTV integration site family (WNT) signaling is pivotal for pre-granulosa cell (pre-GC) activation during PFA. We identified several WNT ligands expressed in pre-GCs that act in an autocrine manner. Inhibition of WNT...

...-posterior axis patterning. Wnt signaling Axin Sea urchin Anterior-posterior axis Animal-vegetal axis Endomesoderm During embryonic development in most bilaterally symmetrical animals (Bilateria), the establishment of the anterior-posterior (AP) and dorsal-ventral (DV) axes are two early...

... by The Company of Biologists Ltd 2021 Summary: To model human birth defects caused by DDX3X mutations, we show that Xenopus DDX3 induces neural crest via the AKT-GSK3β axis, which promotes Wnt signaling and Snai1 stability. DDX3 Neural crest (NC) AKT GSK3β Wnt signaling Xenopus...

... of mesenchymal androgen signaling as cellular niches controlling prostate early development. Androgen signaling Prostate development Wnt signaling Sonic hedgehog signaling Mouse models The prostate develops from the endodermal urogenital sinus (UGS) that is derived from the caudal terminus...

... sustaining the tailbud-derived bipotential neuromesodermal progenitors. Hox genes Brachyury Wnt signaling Neuromesodermal progenitors A hallmark of early embryonic vertebrate development is the progressive formation of the posterior embryonic body from anterior to posterior ( Kimelman...