...; Nicolas Rohner; C. Ben Lovely; Kristen M. Kwan; Alexa Burger; Christian Mosimann ABSTRACT Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible modular system. Here...

...Jennifer Anne Roberts; Irene Miguel-Escalada; Katherine Joan Slovik; Kathleen Theodora Walsh; Yavor Hadzhiev; Remo Sanges; Elia Stupka; Elizabeth Kate Marsh; Jorune Balciuniene; Darius Balciunas; Ferenc Müller Zebrafish transgenesis is increasingly popular owing to the optical transparency...

... of biological systems, the ability to move transgenesis experiments efficiently between organisms becomes essential. DNA constructions used in transgenesis usually contain four elements, including sequences that facilitate transgene genome integration, a selectable marker and promoter elements driving a coding...

... statement The authors declare no competing financial interests. 5 9 2011 © 2011. Transgenesis Er71 ( Etv2 ) knockout Mouse Transcriptional networks, signaling cascades, microRNAs and other factors coordinately regulate stem and progenitor cell populations to give rise...

... recapitulate endogenous expression patterns and levels is often difficult and using large genomic DNA fragments, such as bacterial artificial chromosomes (BACs), makes the process of transgenesis less reliable. Here we provide a detailed protocol that allows for BAC selection and subsequent rapid modification...

... and qualitative analysis of genomic integration events . Genetics 167 , 737 - 746 . Pavlopoulos A. , Oehler S. , Kapetanaki M. G. , Savakis C. ( 2007 ). The DNA transposon Minos as a tool for transgenesis and functional genomic analysis in vertebrates and invertebrates . Genome...

... regulated genes in zebrafish . Dev. Cell 7 , 133 - 144 . Kikuta H. , Kawakami K. ( 2009 ). Transient and stable transgenesis using tol2 transposon vectors . Methods Mol. Biol. 546 , 69 - 84 . Kwan K. M. , Fujimoto E. , Grabher C. , Mangum B. D. , Hardy...

... define Tbx20 as a direct transcriptional target of the BMP/SMAD1 signaling pathway during cardiac maturation. For transient expression in zebrafish, the XTbx20 (–334)-EGFP reporter construct was flanked by Tol2 arms in a pT2 vector for Tol2 transposase-mediated transgenesis ( Fisher et al., 2006...

... for organogenesis, knowledge of the regulation of Wt1 expression is scarce. Here, we have used transgenesis in zebrafish harboring two wt1 genes, wt1a and wt1b , in order to define regulatory elements that drive wt1 expression in the kidney. Stable transgenic lines with approximately 30 kb of the upstream genomic...

... 14 12 2004 ©2005. 2005 Tbx5 Gata4 Nkx2-5 Smooth muscle Transgenesis Knowledge of the regulatory mechanisms underlying cardiac development is essential for understanding the molecular basis of congenital heart defects. During embryonic heart development, differentiation...

..., the potentialities and fate of MH precursors in these conditions have not been directly determined. To follow the dynamics of MH maintenance in vivo, we used artificial chromosome transgenesis in zebrafish to construct lines where egfp transcription is driven by the complete set of regulatory elements of her5...

...-mail: brand@mpi-cbg.de ) 8 4 2002 © 2002. 2002 Pax Transgenesis no isthmus Fgf8 engrailed Zebrafish Danio rerio Cell signaling CNS Midbrain Hindbrain Isthmus Organizer Pattern formation During pattern formation in vertebrate neural development regional gene...

... 2001 © 2001. 2001 Lens Differentiation TGFβ TGFβ receptors Transgenesis Mouse Previous studies have provided evidence that members of the fibroblast growth factor (FGF) and transforming growth factor β (TGFβ) growth factor families play important roles in lens biology. Numerous...

...@nimr.mrc.ac.uk ) 14 03 2000 10 05 2000 © 2000 by Company of Biologists 2000 Brachyury Mesoderm Repression SIP1 Transcriptional regulation Transgenesis Xbra Xenopus The Xenopus blastula is patterned into three domains: the animal pole region, the marginal zone...

.... * These authors contributed equally to this manuscript ‡ Author for correspondence (e-mail: rmg9p@virginia.edu ) 17 10 1997 © 2000 by Company of Biologists 2000 Xenopus tropicalis Crystallin Lens Timing Transgenesis Differentiation We have used Xenopus tropicalis...

.... More recently, greater attention has been focused on the regulatory circuits that control the regionally specific localisation and activity of these molecules. Xenopus Nodal Xnr1 Organizer Endoderm Transgenesis T-box VegT © 2000 by Company of Biologists 2000 03 01 2000...

... embryos. * Author for correspondence (e-mail: jim@nimr.mrc.ac.uk ) 07 09 1999 17 07 1999 © 1999 by Company of Biologists 1999 T-box VegT Endoderm Xenopus laevis Transgenesis Antisense depletion analysis One interpretation of the inability of a single T-box...

... Evolutionary conservation Pancreas Retina Spinal cord Transgenesis The eye forms during vertebrate embryogenesis through a series of inductive interactions involving neuroectoderm, surface ectoderm, neural crest and mesoderm cell populations. This process, which has long served as a paradigm...

... reporter gene, it should be possible to visualize the origin and migration of any lineage-specific progenitor cells in a living embryo. * Author for correspondence (e-mail: slin@mail.mcg.edu ) 06 08 1997 © 1997 by Company of Biologists 1997 transgenesis hematopoiesis zebrafish...

... mouse development. * Author for correspondence (e-mail: thompson@biotec.jouy.inra.fr ) 11 06 1997 © 1995 by Company of Biologists 1995 chromatin histone H4 acetylation HMG-I(Y) scaffold attachment regions transgenesis There are also indications of a more global...