..., immune function and cancer progression. As invasion through BM is often stochastic, capturing gene expression profiles of actively invading cells in vivo remains elusive. Using the stereotyped timing of Caenorhabditis elegans anchor cell (AC) invasion, we generated an AC transcriptome during BM breaching...

... , B. , Gonatopoulos-Pournatzis , T. , Frey , B. , Irimia , M. and Blencowe , B. J. ( 2014 ). Widespread intron retention in mammals functionally tunes transcriptomes . Genome Res. 24 , 1774 - 1786 . 10.1101/gr.177790.114 Chakravarty , A. K. and Shuman , S...

... vertebrate organogenesis. However, little is known about the cellular heterogeneity of the mesenchymal progenitors in early limb buds that ultimately contribute to the chondrogenic condensations prefiguring the skeleton. We combined flow cytometric and transcriptome analyses to identify the molecular...

... single cell RNA sequencing to profile transcriptomes of dissociated cells from the diencephalon of E12.5 mouse embryos. We identified the divergence of different progenitors, intermediate progenitors, and emerging neurons. By mapping the identified cell groups to their spatial origins, we characterized...

.... However, the cellular and molecular basis of tail regeneration in amphioxus remains largely ill-defined. We confirmed that the tail regeneration of amphioxus Branchiostoma japonicum is a vertebrate-like epimorphosis process. We performed transcriptome analysis of tail regenerates, which provided many...

...; Majlinda Lako ABSTRACT The scarcity of embryonic/foetal material as a resource for direct study means that there is still limited understanding of human retina development. Here, we present an integrated transcriptome analysis combined with immunohistochemistry in human eye and retinal samples from 4 to 19...

... organism. This interest has been further fueled by the advent of high-throughput transcriptomic analyses that provide virtual mountains of data. Here, we present N ematostella vectensis Embryogenesis and Regeneration Transcriptomics (NvERTx), a platform for comparing gene expression during embryogenesis...

... a comprehensive Ciona notochord transcriptome. We identify 1364 genes with enriched expression and extensively validate the results by in situ hybridization. These genes are highly enriched for Gene Ontology terms related to the extracellular matrix, cell adhesion and cytoskeleton. Orthologs of 112 of the Ciona...

... Mouse Transcriptome Dosage compensation of X-linked genes is a crucial epigenetic event that occurs at around the peri-implantation stages to allow successful development of female mice. The X inactive-specific transcript ( Xist ) gene encodes a long noncoding RNA that is monoallelically...

...J. Gray Camp; Barbara Treutlein Innovative methods designed to recapitulate human organogenesis from pluripotent stem cells provide a means to explore human developmental biology. New technologies to sequence and analyze single-cell transcriptomes can deconstruct these ‘organoids’ into constituent...

... of mouse embryonic stem cell identity upon differentiation immediately follows collapse of the naïve pluripotency transcription factor circuitry and precedes upregulation of lineage-specific factors. ES cells Rex1 Epiblast Methylome Pluripotency Transcriptome Epiblast cells, founders...

... . Plant Cell   25 , 2798 - 2812 . 10.1105/tpc.113.112227 Feng , B. , Lu , D. , Ma , X. , Peng , Y. , Sun , Y. , Ning , G. and Ma , H. ( 2012 ). Regulation of the Arabidopsis anther transcriptome by DYT1 for pollen development . Plant J.   72 , 612 - 624 . 10.1111...

... 2016. Published by The Company of Biologists Ltd 2016 Summary: Using laser capture micro-dissection followed by array hybridization, the temporal and spatial gene expression changes occurring during rice embryogenesis are captured. Embryogenesis Rice Transcriptome Gene expression...

... the same embryo. As predicted by the transcriptomic data, immunolocalization of EOMES, T brachyury, GDF15 and active β-catenin revealed differential expression among blastomeres of 8- to 10-cell human embryos. The UCSFB lines formed derivatives of the three germ layers and CDX2-positive progeny, from which...

... for correspondence ( irie@bs.s.u-tokyo.ac.jp ; saizo@cdb.riken.jp ) Competing interests The authors declare no competing financial interests. © 2014. Published by The Company of Biologists Ltd 2014 Body plan Evo-devo Evolution Hourglass model Phylotype Transcriptome Animals...

... identified. We have undertaken a transcriptome analysis of mouse limb tendon cells that were isolated at different stages of development based on scleraxis ( Scx ) expression. Microarray comparisons allowed us to establish a list of genes regulated in tendon cells during mouse limb development...

... of transcriptome change in progressively more differentiated neural cell populations in vivo . Microarray data, validated against direct RNA sequencing, identified: (1) a gene cohort characteristic of the multi-potent stem zone epiblast, which contains neuro-mesodermal progenitors that progressively generate...

... requires the identification of crucial differentially expressed control genes and, ideally, the determination of the complete transcriptomes of each individual cell type. Here, we have analyzed the transcriptomes of six major tissues dissected from mid-gestational (TS12) mouse embryos. Approximately one...

... potential of the subgranular zone (SGZ) of the hippocampal dentate gyrus is likely to be regulated by molecular cues arising from its complex heterogeneous cellular environment. Through transcriptome analysis using laser microdissection coupled with DNA microarrays, in combination with analysis of genome...

... and zygotic transcriptomes during this period. Here we use exome sequencing and RNA-seq to achieve such discrimination and in doing so have identified the first zygotic genes to be expressed in the embryo. Our work revealed different profiles of maternal mRNA post-transcriptional regulation prior to zygotic...