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Keywords: Time-lapse microscopy
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Journal Articles
Journal: Development
Development (2017) 144 (7): 1350–1361.
Published: 1 April 2017
... analysis and machine learning methods identify cell divisions oriented by mechanical cues to reduce strain. Image analysis Machine learning Time-lapse microscopy Morphogenesis Oriented cell division Laser ablation Axis elongation is a conserved morphogenetic process in which embryos...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (23): 4151–4157.
Published: 1 December 2015
... is understudied. We recently developed a technique to simultaneously analyse flow dynamics and vascular remodelling by time-lapse microscopy in the capillary plexus of avian embryos and used this to study the hemodynamic environment present during angiogenic sprouting. We found that sprouts always form from...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (23): 4158–4167.
Published: 1 December 2015
... and blood flow in avian embryos allows precise quantification of the hemodynamic parameters that regulate vascular remodelling. Angiogenesis Arteriogenesis Hemodynamics Time-lapse microscopy Micro-particle image velocimetry Computational fluid dynamics Fig. 4. Fluid dynamic analysis...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (11): 2279–2288.
Published: 1 June 2014
.... Inner cell mass Trophectoderm Mouse embryogenesis Pre-implantation lineage specification Time-lapse microscopy The mouse zygote undergoes near synchronous cleavage divisions to give rise to a morula composed of eight roughly spherical blastomeres. These cells become polarised along...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (6): 1381–1391.
Published: 15 March 2014
... attributed. Fgf signaling Wnt signaling Embryonic elongation Segmentation clock Signal gradient Time-lapse microscopy Segmentation of the anteroposterior axis is a widespread feature of animal body plans. Interpretation of positional information from signaling gradients...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (2): 459–470.
Published: 15 January 2013
... temporal resolution and throughput, we trapped cells in hydrogel microwell arrays for time-lapse microscopy ( Gobaa et al., 2011 ; Roccio et al., 2012 ) ( supplementary material Fig. S1A ). Individual microwells can be functionalized with desired biomolecules in order to expose cells to components...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2012) 139 (17): 3242–3247.
Published: 1 September 2012
... Time-lapse microscopy Mounting Light sheet microscopy SPIM Fluorescence microscopy is an invaluable tool for in vivo studies of developmental processes. However, excessive light exposure in commonly employed techniques, such as confocal, two-photon or spinning disc systems, causes...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2011) 138 (13): 2833–2843.
Published: 1 July 2011
... was initiated 1 hour after plating and continued at 10-minute intervals, except for Movie 6 in the supplementary material , which was performed 24 hours after plating. Hemangioblast Hemogenic endothelium Serum-free differentiation Single cell colonial differentiation Time-lapse microscopy...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2009) 136 (18): 3195–3203.
Published: 15 September 2009
... cells Time-lapse microscopy Tissue graft Cre-lox Colon Mouse The enteric nervous system (ENS) is formed primarily from neural crest cells (NCCs) from the post-otic hindbrain (vagal NCCs) and by a smaller contribution from the sacral neural tube (sacral NCCs)( Burns et al., 2000 ). Vagal...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (23): 4613–4617.
Published: 1 December 2006
... signalling pathways can generate cyclopic phenotypes by disrupting normal cell movements or introducing new erroneous behaviours. * Author for correspondence (e-mail: rja46@cam.ac.uk ) 4 10 2006 © 2006. 2006 Zebrafish Time-lapse microscopy Neurulation Cyclopia Quantitative...
Includes: Multimedia, Supplementary data