..., Leydig cells and Tcf21- creER + stromal cells in the testicular interstitium. (A) Confocal imaging of testis sections from 6-week-old Scf GFP mice that were stained with anti-GFP and anti-α-SMA antibodies. Arrows indicate peritubular α-SMA + cells and arrowheads indicate perivascular α-SMA...

...-to-posterior pattern is maintained during ovary differentiation ( Suzuki et al., 2015 ). In contrast, testis differentiation, including upregulation of Sry and Sox9 is reported to occur first in the center of the gonad ( Bullejos and Koopman, 2001 , 2005 ), followed by expansion to the gonadal poles...

... initiation in vitro and isolate pure populations of millions of cells undergoing the mitosis-to-meiosis transition for biochemical assays. Testis Spermatogenesis Meiosis Spermatogonia Spermatocytes National Institutes of Health http://dx.doi.org/10.13039/100000002 HD090083 HD090007...

... were then added to cell lysate overnight at 4°C. Before performing the western blot, beads were washed five times with PBS. The expressions of Sohlh1 and Sohlh2 were detected by in situ hybridization with digoxigenin (DIG)-labeled probes on frozen testis sections. The slides were fixed...

... of proteins mediates movement of specific cargo proteins when bound to karyopherin β. Karyopherin α genes have distinct expression patterns in mouse testis, implying they may have unique roles during mammalian spermatogenesis. Here, we use a loss-of-function approach to determine specifically the role...

... by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Summary: Zebrafish Nedd8 plays an important role in controlling ovarian maturation through suppressing androgen receptor activity. Nedd8 Zebrafish Androgen receptor Ovary Testis Neddylation...

...Aya Uchida; Sadman Sakib; Elodie Labit; Sepideh Abbasi; R. Wilder Scott; T. Michael Underhill; Jeff Biernaskie; Ina Dobrinski ABSTRACT In mammalian testis, contractile peritubular myoid cells (PMCs) regulate the transport of sperm and luminal fluid, while secreting growth factors and extracellular...

.... , Wegner , M. and Scherer , G. ( 2009 ). Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8 . Dev. Biol.   327 , 301 - 312 . 10.1016/j.ydbio.2008.12.011 Becht , E. , McInnes , L. , Healy , J...

...John E. La Marca; Sarah T. Diepstraten; Amy L. Hodge; Hongyan Wang; Adam H. Hart; Helena E. Richardson; W. Gregory Somers ABSTRACT One fundamental property of a stem cell niche is the exchange of molecular signals between its component cells. Niche models, such as the Drosophila melanogaster testis...

...Ellen K. Velte; Bryan A. Niedenberger; Nicholas D. Serra; Anukriti Singh; Lorena Roa-DeLaCruz; Brian P. Hermann; Christopher B. Geyer; Allon Klein; Barbara Treutlein ABSTRACT In the mammalian testis, sustained spermatogenesis relies on spermatogonial stem cells (SSCs); their progeny either remain...

... pathways, including the Egfr/Ras/MAPK pathway ( Biteau and Jasper, 2011 ; Buchon et al., 2010 ; Jiang et al., 2011 ; Xu et al., 2011 ) and, as we discuss below, the JAK/STAT pathway. But how conserved are these functions for JAK/STAT signaling? In the mouse testis, Stat3 is not required...

... relationship between FLCs and ALCs has not been clarified yet. Here, we reveal that a subset of FLCs dedifferentiate at fetal stages to give rise to ALCs at the pubertal stage. Moreover, the dedifferentiated cells contribute to the peritubular myoid cell and vascular pericyte populations in the neonatal testis...

.../ Summary: Dynamic cytoplasmic projections connect mammalian spermatogonia and provide patent connections for the sharing of macromolecules during testis development. TEX14 Intercellular bridge Spermatogenesis Spermatogonia Testis Mouse Baboon The foundation of mammalian spermatogenesis...

.... Additionally, blocking RA signaling in Leydig cells resulted in increased permeability of the blood-testis barrier, decreased levels of the steroidogenic enzyme cytochrome P450 17a1 and decreased testosterone levels. Surprisingly, the epididymides of the mutant mice also displayed an abnormal phenotype...

... cyst stem cells (CySCs) in the Drosophila testis is actively promoted by PI3K/Tor signaling, as CySCs lacking PI3K/Tor activity cannot differentiate properly. We find that an insulin peptide produced by somatic cells immediately outside of the stem cell niche acts locally to promote somatic...

...Chang Liu; Karina Rodriguez; Humphrey H.-C. Yao Testis morphogenesis is a highly orchestrated process involving lineage determination of male germ cells and somatic cell types. Although the origin and differentiation of germ cells are known, the developmental course specific for each somatic cell...

... synthesis and degradation. A group of genes that are required for mRNA splicing and protein translation contributes to both GSC self-renewal and early germ cell differentiation. Loss of genes in the protein degradation pathway in cyst cells leads to testis tumors consisting of overproliferated germ cells...

... by The Company of Biologists Ltd 2016 Summary: This Spotlight revisits a landmark paper in developmental biology, published 25 years ago, that reported the sex reversal of an XX mouse carrying an Sry transgene. Sry Sex determination Transgenic mouse Testis One of the most fundamental...

... development and disease progression. However, its function in adult stem cell maintenance is largely unknown. Here, we report the function of SUMOylation in somatic cyst stem cell (CySC) self-renewal in adult Drosophila testis. The SUMO pathway cell-autonomously regulates CySC maintenance. Reduction...

... immunoprecipitation assays (ChIPs) performed on mouse testis at day 5 using an anti-RARG antibody revealed that RARG is present at the RARE region upstream of the Hist1 cluster, at similar levels to the Stra8 promoter ( Fig. 5 B). Compared with controls, there was a significant decrease in acetylated H4 (H4Ac...