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Development (2003) 130 (23): 5803–5813.
Published: 1 December 2003
... incubation of the slices in 0.5 U/ml PI-PLC (Molecular Probes, Eugene, OR) for 2 hours at 37°C. Some of the slices were fixed immediately after the incubation to examine the effect of PI-PLC. After time-lapse imaging, which normally took 1.5 hours, the slices were fixed and immunostained for Tag1( Yamamoto...
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