.... Using a combination of cell sorting and DNA methyl-seq capture, we generated a stage-specific mapping of DNAme in nine populations of differentiating germ cells from perinatal life to spermiogenesis. DNAme was found to reach its lowest level at gestational day 18, the last demethylated coding regions...

... DOT1L- or MLLT10-deficient mice. These results demonstrate that H3K79 methylation promotes histone replacement during spermiogenesis. Total protein extract from testis or cultured cells was prepared by homogenization in five volumes of protein extraction buffer [62.5 mM Tris-HCl (pH 6.8), 3% SDS, 10...

... exchange, including genes encoding transition proteins, protamines and the testis-specific bromodomain protein BRDT. Spermiogenesis Fertility Histone Chromatin Protamine DOT1L National Institutes of Health DP2HD108774 R01HD098128 G. Harold and Leila Y. Mathers Foundation...

... in spermiogenesis, resulting in reduced H3K27me3 in normal spermatozoa. Therefore, the absence of epigenetic remodelling, presumably mediated by histone turnover during spermiogenesis, leads to dysregulation of chromatin accessibility and transcription in ROSI embryos. Thus, our results unveil a molecular logic...

...Naila Umer; Sharang Phadke; Farhad Shakeri; Lena Arévalo; Keerthika Lohanadan; Gregor Kirfel; Marc Sylvester; Andreas Buness; Hubert Schorle ABSTRACT Profilin 4 ( Pfn4 ) is expressed during spermiogenesis and localizes to the acrosome-acroplaxome-manchette complex. Here, we generated PFN4-deficient...

.... The spermatozoids of bryophytes harbor distinctive morphological characteristics, including a cell body with a helical shape and two flagella. During spermiogenesis, the shape and cellular contents of the spermatids are dynamically reorganized. However, the reorganization patterns of each organelle remain obscure...

... and neurons, developing spermatids display significant uncoupling of transcription and translation for delayed translation. Therefore, spermiogenesis represents an excellent in vivo model for investigating the mechanism underlying uncoupled transcription and translation. Through full-length poly(A) deep...

... of Biologists Ltd 2021 Summary: Analysis of a graded series of KATNB1 loss-of-function models reveals that katanin proteins are required to regulate the vast majority of microtubule-based processes in male germ cell development. Male fertility Male infertility Microtubules Spermiogenesis...

... spermatids, transition from a meiotic gene-expression program to a post-meiotic and spermatid gene expression program related to DNA condensation is impaired, suggesting that EWSR1 plays an important role in regulation of spermiogenesis-related mRNA synthesis necessary for spermatid differentiation...

.... In the present study, we investigated the role of A-kinase anchoring protein 3 (AKAP3) during mouse spermiogenesis, using both mouse genetics and proteomics. It was found that AKAP3 is essential for the formation of the specific subcellular structure of the sperm flagellum, motility of sperm and male fertility...

...-to-protamine exchanges, controls spermiogenesis. However, the underlying mechanisms of this regulation are largely unknown. Here, we report that PHF7, a testis-specific PHD and RING finger domain-containing protein, is essential for histone-to-protamine exchange in mice. PHF7 is specifically expressed during...

.... , Duteyrat , J.-L. , Morlé , L. , Durand , B. , et al. ( 2015 ). RFX2 is a major transcriptional regulator of spermiogenesis . PLoS Genet.   11 , e1005368 . 10.1371/journal.pgen.1005368 Kolas , N. K. , Marcon , E. , Crackower , M. A. , Höög , C. , Penninger , J. M...

...Daoqin Zhang; Dan Xie; Xiwen Lin; Longfei Ma; Jian Chen; Daoqi Zhang; Yang Wang; Shuguang Duo; Yanmin Feng; Chunwei Zheng; Binjie Jiang; Yan Ning; Chunsheng Han ABSTRACT The postmeiotic development of male germ cells, also known as spermiogenesis, features the coordinated expression of a large...

...-regulated mechanism generating asymmetrical division in cortical precursors . Cell Rep.   6 , 400 - 414 . 10.1016/j.celrep.2013.12.026 Fabian , L. and Brill , J. A. ( 2012 ). Drosophila spermiogenesis: Big things come from little packages . Spermatogenesis   2 , 197 - 212 . 10.4161...

... function for CFAP157 and suggests that this novel FOXJ1 effector is part of a mechanism that acts during spermiogenesis to suppress the formation of supernumerary axonemes and ensures a correct ultrastructure. * Author for correspondence ( gossler.achim@mh-hannover.de ) Competing interests...

...Wei Li; Waixing Tang; Maria E. Teves; Zhengang Zhang; Ling Zhang; Hongfei Li; Kellie J. Archer; Darrell L. Peterson; David C. Williams, Jr; Jerome F. Strauss, III; Zhibing Zhang A key event in the process of spermiogenesis is the formation of the flagella, which enables sperm to reach eggs...

... with the failure of acrosomal expansion during spermiogenesis and resulted in the degeneration and disappearance of the acrosome in mature spermatozoa. Thus, these findings clarify part of the cascade leading to globozoospermia. * Author for correspondence ( okabe@biken.osaka-u.ac.jp ) Competing...

... Spermiogenesis Mouse Spermatozoon is doubtless one of the most specialized cells in mammals and the description and understanding of the molecular aspects of spermiogenesis represents a very challenging task. The acrosome, a giant vesicle of secretion tightly bound to the nucleus via the acroplaxome...

... previously ( Chung et al., 2005 ). Significant differences were assessed by statistical analysis using Student's paired t -test using GraphPad Analysis software. * Author for correspondence (e-mail: djw3@columbia.edu ) 14 4 2009 © 2009. 2009 Retinoid signaling Spermiogenesis...

...Jing Zhou; Ya-Rui Du; Wei-Hua Qin; Ye-Guang Hu; Yan-Nv Huang; Lan Bao; Daishu Han; Ahmed Mansouri; Guo-Liang Xu During spermiogenesis, round spermatids are converted into motile sperm in mammals. The mechanisms responsible for sperm morphogenesis are poorly understood. We have characterized a novel...