... individualization by promoting local accumulation of the E3 ubiquitin ligase inhibitor Soti. Our results further extend the diversity of non-canonical eIF4Es that carry out distinct spatiotemporal roles during spermatogenesis. * Present address: Clarivate Analytics, 150 King St. W, Toronto, Ontario, M5H 1J9...

.... Summary: Non-dividing niche cells in the adult Drosophila testis, which are able to divide to replace missing stem cells, are unable to replace themselves. Stem cell Niche Spermatogenesis Cell ablation GeneSwitch Johns Hopkins University http://dx.doi.org/10.13039/100007880...

... of spermatogonia, the majority of models in the literature come with limitations, including expression of fluorophores in only a subset of spermatogonia (e.g. SSCs and early progenitors during the first wave of spermatogenesis; Chan et al., 2014 ; Helsel et al., 2017 ) or infrequency of genetically appropriate...

... characterize two suppressors of memi-1(sb41) : gskl-1 and gskl-2 . Both genes encode functionally redundant sperm glycogen synthase kinase, type 3 (GSK3) protein kinases. Loss of both genes causes defects in male spermatogenesis, sperm pseudopod treadmilling and paternal-effect embryonic lethality. The two...

.... , Keeney , S. , Flaus , A. , Owen-Hughes , T. et al. ( 2013 ). Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis . PLoS Genet. 9 , e1003945 . 10.1371/journal.pgen.1003945 Gévry , N...

...Jian Chen; Chenxu Gao; Xiwen Lin; Yan Ning; Wei He; Chunwei Zheng; Daoqin Zhang; Lin Yan; Binjie Jiang; Yuting Zhao; Md Alim Hossen; Chunsheng Han ABSTRACT Spermatogonial differentiation and meiotic initiation during spermatogenesis are tightly regulated by a number of genes, including those...

... and for axoneme stability, and is therefore crucial for spermatogenesis. Cilia Flagella MMAF Spermatogenesis Male infertility Radial spoke CSC National Key Research and Development Program of China 2016YFA0500902 Natural Science Foundation of China http://dx.doi.org/10.13039...

... acrosome biogenesis in spermatogenesis have been well described, the molecular mechanism underlying this process is still largely unknown. Family with sequence similarity 71, member F1 and F2 (FAM71F1 and FAM71F2) are testis-enriched proteins that contain a RAB2B-binding domain, a small GTPase involved...

...Na Liu; Fatimunnisa Qadri; Hauke Busch; Stefanie Huegel; Gabin Sihn; Ilya Chuykin; Enno Hartmann; Michael Bader; Franziska Rother ABSTRACT Spermatogenesis is driven by an ordered series of events, which rely on trafficking of specific proteins between nucleus and cytoplasm. The karyopherin α family...

... in Drosophila , Orb2, is a principal player in spermatogenesis. It is required for meiosis and spermatid differentiation. During the later process, orb2 mRNA and protein are localized within the developing spermatid. To evaluate the role of the orb2 mRNA 3′UTR in spermatogenesis, we used the CRISPR/Cas9 system...

...Grigor Varuzhanyan; Mark S. Ladinsky; Shun-ichi Yamashita; Manabu Abe; Kenji Sakimura; Tomotake Kanki; David C. Chan ABSTRACT Male germline development involves choreographed changes to mitochondrial number, morphology and organization. Mitochondrial reorganization during spermatogenesis...

...; Kazuyuki Ohbo ABSTRACT During spermatogenesis, intricate gene expression is coordinately regulated by epigenetic modifiers, which are required for differentiation of spermatogonial stem cells (SSCs) contained among undifferentiated spermatogonia. We have previously found that KMT2B conveys H3K4me3...

...Xiaoli Wang; Yujiao Wen; Jin Zhang; Grace Swanson; Shuangshuang Guo; Congcong Cao; Stephen A. Krawetz; Zhibing Zhang; Shuiqiao Yuan ABSTRACT Mitochondria play a crucial role in spermatogenesis and are regulated by several mitochondrial fusion proteins. However, their functional importance...

... is identified as an essential factor for C. elegans spermatogenesis, functioning with the kinase SPE-6 to sequester the cytoskeletal element MSP into fibrous bodies. Major sperm protein Intrinsically disordered protein Caenorhabditis elegans Spermatogenesis Cytoskeletal assembly spe-18...

... mutants. In contrast to bmpr2a , the loss of bmpr2b ( bmpr2b −/− ) had no effect on males, which showed normal testicular development and spermatogenesis. By contrast, the female bmpr2b mutant showed severe reproductive defects. The mutant females were infertile because the follicles could grow only...

...James Matthew Ragle; Abigail L. Aita; Kayleigh N. Morrison; Raquel Martinez-Mendez; Hannah N. Saeger; Guinevere A. Ashley; Londen C. Johnson; Katherine A. Schubert; Diane C. Shakes; Jordan D. Ward ABSTRACT In sexually reproducing metazoans, spermatogenesis is the process by which uncommitted germ...

...-seq Germ cells Proliferation Differentiation Spermatogenesis Drosophila Maintenance of a multicellular organism during homeostasis and tissue repair requires active replenishment of depleted or injured cells during aging and regeneration. Adult stem cells fulfill this requirement owing...

...Guihua Du; Xinrui Wang; Mengcheng Luo; Weiya Xu; Tao Zhou; Mei Wang; Luping Yu; Lufan Li; Li'e Cai; P. Jeremy Wang; John Zhong Li; Jon M. Oatley; Xin Wu ABSTRACT Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells...

... with spermatogenesis arrested at MII. Knockout of Cxxc1 led to a decrease in the H3K4me3 level from the pachytene to the MII stage and caused transcriptional disorder. Many spermatogenesis pathway genes were expressed early leading to abnormal acrosome formation in arrested MII cells. In meiotic prophase, deletion...

... of Drosophila gametogenesis, spermatocytes undergo robust gene transcription and store many transcripts in the cytoplasm in a repressed state, until translational activation of select mRNAs in later steps of spermatogenesis. Here, we characterize the Drosophila Doublefault (Dbf) protein as a C2H2 zinc-finger...