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Keywords: Single cell RNA sequencing
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Journal Articles
Journal: Development
Development (2022) 149 (20): dev200693.
Published: 30 August 2022
... expanded into organoids that possessed NKX2-1 + /CPM + BTP-like cells, which increased in number over time. Single cell RNA-sequencing analysis revealed a high degree of transcriptional similarity between induced BTPs (iBTPs) and in vivo BTPs. Using FACS, iBTPs were purified and expanded as induced bud tip...
Includes: Supplementary data
Journal Articles
In collection:
Neural development
Journal: Development
Development (2022) 149 (13): dev200448.
Published: 4 July 2022
... developed a more complex binary vomeronasal system, containing vomeronasal sensory neurons (VSNs) expressing receptors of either the V1R or V2R family. In rodents, V1R/apical and V2R/basal VSNs originate from a common pool of progenitors. Using single cell RNA-sequencing, we identified differential...
Includes: Supplementary data
Journal Articles
In collection:
Neural development
Journal: Development
Development (2020) 147 (6): dev184143.
Published: 16 March 2020
.... In zebrafish, there is spatial patterning of neurogenesis in which non-neurogenic zones form at boundaries and segment centres, in part mediated by Fgf20 signalling. To further understand the control of neurogenesis, we have carried out single cell RNA sequencing of the zebrafish hindbrain at three different...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (23): dev182097.
Published: 5 December 2019
... of FLK1 + mesoderm generation and its allocation to various cell fates remain obscure. Recent single cell RNA-sequencing studies of early embryos or in vitro -differentiated human embryonic stem (ES) cells have provided unprecedented information on the spatiotemporal resolution of cells in embryogenesis...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (12): dev174888.
Published: 17 June 2019
... the fusion site to allow mesenchyme continuity. Although several genes required for fusion have been identified, an integrated molecular and cellular description of the overall process is lacking. Here, we employ single cell RNA sequencing of the developing mouse face to identify ectodermal, mesenchymal...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (12): dev173849.
Published: 17 June 2019
...) reporter mouse line, that closely mirrors the transient endogenous Ngn3 protein expression. To define an in vivo roadmap of endocrinogenesis, we performed single cell RNA sequencing of 36,351 pancreatic epithelial and NVF + cells during secondary transition. This allowed Ngn3 low endocrine progenitors...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (12): dev173476.
Published: 14 June 2019
... exhibit distinct patterns of gene expression, which are thought to contribute to heart development and morphogenesis. Single cell RNA sequencing allows genome-wide analysis of gene expression at the single cell level. Here, we have analyzed cardiac cells derived from early stage developing hearts...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (12): dev173047.
Published: 12 March 2019
.../ Summary: Transcriptomic single cell analysis of postnatal developing heart valves identifies novel cell populations and uncovers interstitial cell heterogeneity. Heart valve Development Single cell RNA sequencing Valve cell lineages Cardiovascular science Mature heart valves...
Includes: Supplementary data