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Keywords: Single cell
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Journal Articles
In collection:
Plant development
Journal: Development
Development (2022) 149 (6): dev200179.
Published: 14 March 2022
...Mingyuan Zhu; Isaiah W. Taylor; Philip N. Benfey ABSTRACT Understanding the development of tissues, organs and entire organisms through the lens of single-cell genomics has revolutionized developmental biology. Although single-cell transcriptomics has been pioneered in animal systems, from...
Journal Articles
Journal: Development
Development (2022) 149 (2): dev199853.
Published: 28 January 2022
...; Nikolay Ninov ABSTRACT Regeneration-competent species possess the ability to reverse the progression of severe diseases by restoring the function of the damaged tissue. However, the cellular dynamics underlying this capability remain unexplored. Here, we have used single-cell transcriptomics to map de...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (23): dev199465.
Published: 14 December 2021
... sorted using an 85 µm nozzle into Eppendorf tubes that had been pre-coated with PBS/2% BSA. Approximately 2500 sorted cells were directly loaded in sheath fluid onto one lane of a Chromium 10X chip (10X Genomics) and libraries prepared using the normal workflow for Single Cell 3´ v3 libraries (10X...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (12): dev188367.
Published: 25 June 2021
... to organoids, single-cell technology and clinical applications. Funding C.B. is supported by Austrian Science Fund (FWF) (P30599 and P34123). F.T.M. is a New York Stem Cell Foundation Robertson Investigator (NYSCF-R-156), and is supported by the Wellcome Trust and Royal Society (211221/Z/18/Z...
Journal Articles
Journal: Development
Development (2019) 146 (24): dev174441.
Published: 13 December 2019
... the transcription factors TCF21 and WT1. Here, we show that this cell heterogeneity is conserved in human epicardium, regulated by BNC1 and associated with cell fate and function. Single cell RNA sequencing of epicardium derived from human pluripotent stem cells (hPSC-epi) revealed that distinct epicardial...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (12): dev176727.
Published: 27 June 2019
...Urs Mayr; Denise Serra; Prisca Liberali; Allon Klein; Barbara Treutlein ABSTRACT Complex 3D tissues arise during development following tightly organized events in space and time. In particular, gene regulatory networks and local interactions between single cells lead to emergent properties...
Journal Articles
Journal: Development
Development (2019) 146 (12): dev169748.
Published: 27 June 2019
...Samantha A. Morris; Allon Klein; Barbara Treutlein ABSTRACT Fueled by recent advances in single cell biology, we are moving away from qualitative and undersampled assessments of cell identity, toward building quantitative, high-resolution cell atlases. However, it remains challenging to precisely...
Journal Articles
Journal: Development
Development (2019) 146 (12): dev169730.
Published: 27 June 2019
... populations per experiment. A new generation of tools that use molecular recording methods integrated with single cell profiling technologies may provide a solution. Here, we summarize recent breakthroughs in these technologies, outline experimental and computational challenges, and discuss biological...
Journal Articles
Journal: Development
Development (2019) 146 (12): dev172940.
Published: 3 June 2019
... in individual cells during this transition is unclear. Using a FRET-based biosensor, we monitored ERK signalling dynamics of single mouse ESCs during differentiation. ERK activity was highly heterogeneous, with considerable variability in ERK signalling between single cells within ESC colonies. Different...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (6): dev174953.
Published: 27 March 2019
... of spermatogenesis. However, a systematic understanding of this process is still limited. To resolve cellular and molecular heterogeneity in this process, we profiled single cell transcriptomes of undifferentiated germ cells from neonatal mouse testes and employed unbiased clustering and pseudotime ordering analysis...
Includes: Supplementary data
Journal Articles
In collection:
Human development
Journal: Development
Development (2018) 145 (16): dev164038.
Published: 30 August 2018
... single-cell transcriptomics to characterize gene expression in different cell populations, and to study individual cell dynamics and lineage trajectories during development. Single-cell transcriptome analyses of 6414 cells from five individual specimens identified 11 initial clusters of specific renal...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (3): dev158501.
Published: 7 February 2018
...Giuliano G. Stirparo; Thorsten Boroviak; Ge Guo; Jennifer Nichols; Austin Smith; Paul Bertone ABSTRACT Single-cell profiling techniques create opportunities to delineate cell fate progression in mammalian development. Recent studies have provided transcriptome data from human pre-implantation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (19): 3625–3632.
Published: 1 October 2017
...Mike Adam; Andrew S. Potter; S. Steven Potter Single-cell RNA-seq is a powerful technique. Nevertheless, there are important limitations, including the technical challenges of breaking down an organ or tissue into a single-cell suspension. Invariably, this has required enzymatic incubation at 37°C...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (1): 17–32.
Published: 1 January 2017
... the tantalizing prospect of cataloging cell type diversity and developmental dynamics in a quantitative and comprehensive manner. Here, we review how single-cell RNA sequencing has provided key insights into mammalian developmental and stem cell biology, emphasizing the analytical approaches that are specific...
Journal Articles
Journal: Development
Development (2016) 143 (16): 2877–2881.
Published: 15 August 2016
... and adult stem cell biology were discussed, including their respective niches in ageing, disease and regeneration. Many presentations focused on emerging technologies, including single-cell analysis, in vitro organogenesis and direct reprogramming. Here, we summarize the data presented at this exciting...
Journal Articles
Journal: Development
Development (2015) 142 (11): 2014–2025.
Published: 1 June 2015
... modeling predicted that a small fraction of lead cells that detect directional information is optimal for successful stream migration. Single-cell profiling then revealed a unique molecular signature that is consistent and stable over time in a subset of lead cells within the most advanced portion...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (1): 216–225.
Published: 1 January 2013
... financial interests. 25 9 2012 © 2013. Published by The Company of Biologists Ltd RNA visualization Cell state Differentiation Fluorescent in situ hybridization Single cell Single molecule The primary unit of biological control is the cell. Measurement...
Includes: Supplementary data