.... Handling Editor: James Wells * Author for correspondence ( ehashino@iu.edu ) Lilly Endowment http://dx.doi.org/10.13039/100006976 National Institutes of Health http://dx.doi.org/10.13039/100000002 Highlighted Article: Longitudinal scRNA-seq analysis of human...

... (scRNA-seq) analysis, and also compared their transcriptomes with embryonic RGCs. Our approach enabled us to: (1) investigate why, despite Pten KO in all RGC types, some do not regenerate axons, others (such as αRGCs) regenerate short-distance axons ( Jacobi et al., 2022 ), and only a rare subset...

... Nitex mesh, counted on a hemocytometer and diluted to reach the appropriate concentration for the scRNA-seq protocol. Equal numbers of embryos were used in each time point and at no time were cells or embryos pelleted in a centrifuge ( Oulhen et al., 2019 ). Evidence suggests...

... identified regulatory genes of cell differentiation in poplar apex, establishing a comparison between a model annual herbaceous and a woody perennial plant species. Populus Vascular development scRNA-seq Developmental trajectory Shoot apex Cell differentiation U.S. Department of Energy...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Summary: This study establishes a transcriptional atlas of the cell fate decisions underlying LPM specification, subdivision and development of the chicken forelimbs. EMT LPM Limb Chicken scRNA...

... four branchial arches that may be mined for insights into cell fate decisions. Previous knowledge derived from our sc-qPCR and scRNA-seq profiling of purified neural crest cells combined here with the innovative label-free, non-isolation cell profiling of the entire branchial arches provided confidence...

...Abdull J. Massri; Laura Greenstreet; Anton Afanassiev; Alejandro Berrio; Gregory A. Wray; Geoffrey Schiebinger; David R. McClay ABSTRACT Using scRNA-seq coupled with computational approaches, we studied transcriptional changes in cell states of sea urchin embryos during development to the larval...

... with chondrogenic progression inhibit AER cell formation and restrict limb regeneration potential. Limb regeneration Xenopus Apical-ectodermal-ridge scRNA-Seq Ex vivo limbs Amphibian limb regeneration relies on a specialized wound epidermis (also known as the apical-epithelial-cap, AEC...

... and involvement of alx3 in skeletal cell morphology and differentiation timing affecting neurocranium shape. Zebrafish Craniofacial skeleton Neurocranium scRNA-seq Differentiation Neural crest cells Cartilage Bone Alx The vertebrate head skeleton has many cartilaginous and bony elements...

...: The transcription-factor Runx1 orchestrates the injury response of many cardiac cell types, balancing collagen and fibrin deposition and clearance, as well as affecting myocyte proliferation and survival in zebrafish. Heart Regeneration Runx1 ScRNA-seq Zebrafish Heart regeneration potential varies...

... profiles, identifying co-regulated genes that mediate a temporal programme of neuronal subtype specification. Spinal cord Neural development Neural tube ScRNA-seq Single cell transcriptomics Neuronal circuits in the spinal cord receive and process incoming sensory information from...