..., the effects of morphological changes on intercellular signaling remain to be fully elucidated. In this study, we examined the effect of morphological changes in Wnt-producing cells on intercellular signaling in the spinal cord. After mid-gestation, roof-plate cells stretched along the dorsoventral axis...

... trunk NC derivatives, including SG, SC precursors, DRG and melanocytes ( n =6/6, Fig. 1D , insets I-IV, 1E,H) as well as in definitive roof plate (RP) cells that co-expressed the RP marker MafB ( Lecoin et al., 2004 ; Liem et al., 1997 ) ( Fig. 1E-E″ ). By contrast, when given at 30 ss, the progeny...

... activity is directed dorsally to the roof plate and dorsal-most (dI1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights...

... that it fails to develop because reducing FGF signaling perturbs the balance between vermis and roof plate development in rhombomere 1. We suggest a molecular explanation for this phenomenon by providing evidence that FGF signaling functions to inhibit the BMP signaling that promotes roof plate development...

...Nina L. Hunter; Susan M. Dymecki Both hindbrain roof plate epithelium (hRPe) and hindbrain choroid plexus epithelium (hCPe) produce morphogens and growth factors essential for proper hindbrain development. Despite their importance, little is known about how these essential structures develop...

... of the neural tube. Mechanisms specifying distinct populations within the early cerebellar anlage are less defined. Using a newly developed gene expression map of the early cerebellar anlage, we demonstrate that secreted signals from the rhombomere 1 roof plate are both necessary and sufficient...

...Paula Alexandre; Isabelle Bachy; Morgane Marcou; Marion Wassef The roof plate (RP) of the midbrain shows an unusual plasticity, as it is duplicated or interrupted by experimental manipulations involving the mid/hindbrain organizer or FGF8. In previous experiments, we have found that FGF8 induces...

... double- and Hes1;Hes3;Hes5 triple-null embryos( Fig. 6K,L ). In addition, we found that neurons (TuJ1-positive cells) are ectopically differentiated in the roof plate and the floor plate of Hes1;Hes3;Hes5 triple-null embryos(data not shown). Because cell death is not increased in Hes-null mice...

... depends on DM cells in a non-cell-autonomous fashion. * Author for correspondence (e-mail: emonuki@uci.edu ) 23 5 2005 ©2005. 2005 Roof plate Fate map Genetic ablation Bmp Gdf7 Transgenic mice Cre recombinase Diphtheria toxin Mouse The choroid plexus (CP) produces...

...Victor V. Chizhikov; Kathleen J. Millen Numerous studies have identified the roof plate as an embryonic signaling center critical for dorsal central nervous system patterning, but little is known about mechanisms that control its formation and its separation from clonally related neural crest cells...

...Ying Liu; Amy W. Helms; Jane E. Johnson Patterning of the dorsal neural tube involves Bmp signaling, which results in activation of multiple pathways leading to the formation of neural crest,roof plate and dorsal interneuron cell types. We show that constitutive activation of Bmp signaling at early...

..., is required for the migration of cells forming the roof plate of the neural tube and, subsequently, for neural crest migration. These cells are properly determined but remain at their site of origin. Consistent with a possible role in cell movement, Vg1 RBP asymmetrically localizes to extended processes...

... domain located at the mid/hindbrain boundary produce the roof plate of the mid/hindbrain domain. Cells migrating anteriorly from this region populate the caudal midbrain roof plate whereas cells migrating posteriorly populate the cerebellar roof plate. In addition, the adjacent paramedial isthmic...

... of transplantation and ablation experiments in avian embryos, we show here that, in the caudal midbrain, a restricted dorsal domain of the isthmic organizer, that we call the isthmic node, is both necessary and sufficient for the formation and positioning of the roof plate, a signaling structure that marks...

... and Bmp7 are required for cushion formation and septation in the developing mouse heart. Dev. Biol. 235 , 449 -466. Lee, K. J., Dietrich, P. and Jessell, T. M. ( 2000 ). Genetic ablation reveals that the roof plate is essential for dorsal interneuron specification. Nature 403 , 734 -740. Lee...

... by expression of dopamine-β-hydroxylase and tyrosine hydroxylase. Bmp5 is expressed in the dorsal neuroepithelium in proximity to Phox2 -expressing cells. BMP inhibition in stage 10 chick embryos resulted in the lack of LC neurones or in their generation at the dorsal midline, and loss of roof plate and rhombic...

...Kenji Shimamura; Shinji Hirano; Andrew P. McMahon; Masatoshi Takeichi ABSTRACT E-cadherin is transiently expressed in local regions of the embryonic mouse brain, which include several patchy areas on the mesencephalon and diencephalon and their roof plate and part of cerebellar rudiments...