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Keywords: RNAi
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Journal Articles
Journal: Development
Development dev.200692.
Published: 25 July 2022
... by The Company of Biologists Ltd 2022 http://www.biologists.com/user-licence-1-1/ C. elegans germline RNAi Genome surveillance siRNA suppression Transposon Office of Extramural Research, National Institutes of Health https://dx.doi.org/10.13039/100006955 R01GM111752 Target...
Journal Articles
Journal: Development
Development (2015) 142 (3): 597–606.
Published: 1 February 2015
... tissue. More recently, large collections of RNAi lines or cDNAs expressed under Gal4/ UAS control have been used to alter gene expression uniformly in specific tissues. However, these newer approaches are not easily combined with the efficient generation of genetic mosaics. The CoinFLP system described...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (12): 2494–2505.
Published: 15 June 2014
... interference (RNAi), was performed in pre-migratory FNC cells. Soon after electroporation of RNAi molecules, Smad1 inactivation abolished the expression of Foxg1 in the chick telencephalon, resulting in dramatic microcephaly and partial holoprosencephaly. In addition, the depletion of Foxg1 activity altered...
Journal Articles
Journal: Development
Development (2012) 139 (8): 1399–1404.
Published: 15 April 2012
... to megagametogenesis. ARGONAUTE5 ( AGO5 ), a putative effector of small RNA (sRNA) silencing pathways, was found to be expressed around reproductive cells during megasporogenesis, and a novel semi-dominant ago5-4 insertion allele showed defects in the initiation of megagametogenesis. Expression of a viral RNAi...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2011) 138 (10): 1893–1902.
Published: 15 May 2011
... about how cell numbers are controlled in specific cell cohorts along the dorsoventral axis of the neural tube. Here, we show that FatJ cadherin, identified in a large-scale RNA interference (RNAi) screen of cadherin genes expressed in the neural tube, is localised to progenitors in intermediate regions...
Includes: Supplementary data
Journal Articles
Journal: Development
Series: Primer Series
Development (2010) 137 (21): 3535–3543.
Published: 1 November 2010
... for subsequent transformation with the same selectable marker ( Schaefer and Zryd, 2001 ). Frequently, transforming DNA * Author for correspondence ( bezanilla@bio.umass.edu ) Competing interests statement © 2010. Physcomitrella patens RNAi Homologous recombination...
Journal Articles
Journal: Development
Development (2010) 137 (20): 3469–3476.
Published: 15 October 2010
... play a central role in establishing proper fates along the entire DV axis in this animal; laser ablation of midline cells causes a failure to form neurogenic ectoderm and Ph-sim RNAi results in severely dorsalized embryos lacking both neurogenic ectoderm and the appendage-bearing lateral ectoderm...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2010) 137 (18): 3057–3066.
Published: 15 September 2010
... of the animals and repression of aboral structures (i.e. stolons). The expression of Wnt3, Tcf and Brachyury was upregulated and became ubiquitous. Rescue experiments using Tcf RNAi resulted in normal metamorphosis and quantitatively normal Wnt3 and Brachyury expression. Isolated, decapitated polyps regenerated...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2010) 137 (8): 1263–1273.
Published: 15 April 2010
... of the availability of parental RNAi in the spider Achaearanea tepidariorum to carry out a function-based screen for genes involved in cell migration during embryogenesis. The knockdown of some genes affected CM cell migration but had no apparent effects on earlier events, such as blastoderm, germ disc and CM cell...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2008) 135 (8): 1439–1449.
Published: 15 April 2008
...Jianwu Bai; Richard Binari; Jian-Quan Ni; Marina Vijayakanthan; Hong-Sheng Li; Norbert Perrimon To facilitate the genetic analysis of muscle assembly and maintenance, we have developed a method for efficient RNA interference (RNAi) in Drosophila primary cells using double-stranded RNAs (dsRNAs...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2008) 135 (5): 983–993.
Published: 1 March 2008
.... In addition, DEPS-1 is required for RNA interference(RNAi) of germline-expressed genes, possibly because DEPS-1 promotes the accumulation of RDE-4, a dsRNA-binding protein required for RNAi. A genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1...
Journal Articles
Journal: Development
Development (2006) 133 (15): 2973–2981.
Published: 1 August 2006
..., rather than being a component of this signal. siRNA RNAi Heterochrony Arabidopsis References Adenot, X., Elmayan, T., Lauressergues, D., Boutet, S., Bouche,N., Gasciolli, V. and Vaucheret, H. ( 2006 ). DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7...
Journal Articles
Journal: Development
Development (2006) 133 (12): 2347–2357.
Published: 15 June 2006
... , was specifically expressed at the dorsal side of each limb bud that had started to extend( Fig. 5J ), as described for Cupiennius omb ( Prpic et al.,2003 ). In At-sog RNAi embryos exhibiting severe phenotypes, the domains of At-omb expression were fused to cover the entire width of the germ band ( Fig. 5L...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (7): 1263–1275.
Published: 1 April 2006
... is poorly understood. In C. elegans , the body wall muscles (BWMs)also have plasma membrane extensions called muscle arms that are guided to the motor axons where they form the postsynaptic element of the NMJ. To investigate the regulation of muscle membrane extension, we screened 871 genes by RNAi...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (4): 711–723.
Published: 15 February 2006
... and identified distinct classes of upregulated genes during this process. Fifty-seven genes were then tested functionally by RNAi. We found six genes affecting various aspects of cellular architecture: membrane growth, organelle transport or organisation and junction assembly. We focus here on charleston ( char...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (10): 2463–2474.
Published: 15 May 2005
... Embryo Motoneurons RNAi Optical projection tomography The T-box transcription factors are a family of developmentally regulated DNA-binding proteins that play important roles in organogenesis( Papaioannou, 2001 ). Their function is conserved throughout evolution, and they participate...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (9): 2081–2092.
Published: 1 May 2005
... insect Oncopeltus fasciatus . We find that in Oncopeltus, even-skipped striped expression initiates in a segmental and not pair-rule pattern. Weak RNAi suppression of Oncopeltus even-skipped shows no apparent pair-rule like phenotype, while stronger RNAi suppression shows deletion of nearly the entire...
Journal Articles
Journal: Development
Development (2005) 132 (9): 2069–2079.
Published: 1 May 2005
..., Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA 14 2 2005 ©2005. 2005 We also observed that Gb'Antp was expressed in the prospective labial and thoracic segments in the early stages( Fig. 7A ). In the shortened and widened Gb'hb RNAi embryos...
Journal Articles
Journal: Development
Development (2005) 132 (8): 1863–1874.
Published: 15 April 2005
... RNA interference (RNAi), we demonstrate that gene silencing of DjPum dramatically reduces the number of neoblasts, thus supporting the intriguing hypothesis that stem cell maintenance may be an ancestral function of PUF proteins. † Author for correspondence (e-mail: gremigni@biomed.unipi.it...
Journal Articles
Journal: Development
Development (2004) 131 (18): 4567–4579.
Published: 15 September 2004
... the blastoderm and germband stages of embryogenesis. In order to investigate the function of Krüppel in Oncopeltus segmentation, we generated knockdown phenotypes using RNAi. Loss of Krüppel activity in Oncopeltus results in a large gap phenotype, with loss of the mesothoracic through fourth abdominal segments...