... Neuromesodermal progenitors Segmentation clock Embryo Presomitic mesoderm The Myc proto-oncogene family is one of the most exhaustively studied families of vertebrate genes ( Eilers and Eisenman, 2008 ; Meyer and Penn, 2008 ). Since the discovery of cMyc (in chick) ( Alitalo et al., 1983 ; Watson...

... Body skeletal muscles derive from the paraxial mesoderm, which forms in the posterior region of the embryo. Using microarrays, we characterize novel mouse presomitic mesoderm (PSM) markers and show that, unlike the abrupt transcriptome reorganization of the PSM, neural tube differentiation...

... and of the transcriptional co-activator SAGA, is required for the assembly of these complexes in the mouse embryo. We performed Taf10 conditional deletions during mesoderm development and show that Taf10 loss in the presomitic mesoderm (PSM) does not prevent cyclic gene transcription or PSM segmental patterning, whereas...

...Richa Sharma; Maxwell E. R. Shafer; Eric Bareke; Mathieu Tremblay; Jacek Majewski; Maxime Bouchard ABSTRACT Caudal somites are generated from a pool of progenitor cells located in the tailbud region. These progenitor cells form the presomitic mesoderm that gradually differentiates into somites...

... and presomitic mesoderm. In the presence of RA, RARγ2 serves as an activator, facilitating somite differentiation. Treatment with an RARγ-selective inverse agonist (NRX205099) or overexpression of dominant-negative RARγ increases the expression of posterior Hox genes and that of marker genes for presomitic...

...Laurent Yvernogeau; Gwenola Auda-Boucher; Josiane Fontaine-Perus We have combined the use of mouse genetic strains and the mouse-into-chicken chimera system to determine precisely the sequence of forelimb colonization by presomitic mesoderm (PSM)-derived myoblasts and angioblasts, and the possible...

..., suggesting the importance of the Notch on/off state for boundary formation. However, a recent study has shown that mouse embryos expressing Notch-intracellular domain (NICD) throughout the presomitic mesoderm (PSM) can still form more than ten somites, indicating that the NICD on/off state is dispensable...

...Masayuki Oginuma; Yasutaka Niwa; Deborah L. Chapman; Yumiko Saga The metameric structures in vertebrates are based on the periodicity of the somites that are formed one by one from the anterior end of the presomitic mesoderm (PSM). The timing and spacing of somitogenesis are regulated...

...Pedro Rifes; Lara Carvalho; Catarina Lopes; Raquel P. Andrade; Gabriela Rodrigues; Isabel Palmeirim; Sólveig Thorsteinsdóttir The absence of ectoderm impairs somite formation in cultured presomitic mesoderm (PSM) explants, suggesting that an ectoderm-derived signal is essential for somitogenesis...

.... Huppert, S. S., Ilagan, M. X., De Strooper, B. and Kopan, R. ( 2005 ). Analysis of Notch function in presomitic mesoderm suggests a gamma-secretase-independent role for presenilins in somite differentiation. Dev. Cell 8 , 677 -688. Iulianella, A., Melton, K. R. and Trainor, P. A. ( 2003...

...Birgit Aerne; David Ish-Horowicz Segmentation in vertebrate embryos is controlled by a biochemical oscillator (`segmentation clock') intrinsic to the cells in the unsegmented presomitic mesoderm, and is manifested in cyclic transcription of genes involved in establishing somite polarity...

... , but not Myf5 expression;however, we show that the source of the Wnt signal is intrinsic to the mesoderm. Gain- and loss-of-function experiments indicate that Wnt5b ,which is expressed in the presomitic mesoderm, represents the MyoD -activating cue. Despite Wnt5b expression in the presomitic mesoderm, MyoD...

... to all segments, from their most anterior to their most posterior borders. Therefore, it appears that mediolateral regionalisation of myotomal precursor cells is a property established before bilateralisation of the presomitic mesoderm and that coherent clonal growth accompanies cell dispersion along...

... Presomitic mesoderm Somitogenesis Notch signaling her1 her7 Zebrafish Segmentation of the body is a feature of development common to many animals. There are at least two modes of segmentation: the division of an existing tissue and the sequential division of a continuously growing tissue...

... evidence indicates that a molecular oscillator, the ‘segmentation clock’, operates in the presomitic mesoderm (PSM) to direct periodic expression of c-hairy1 and lunatic fringe (l-fng) . Here, we report the identification and characterisation of a second avian hairy -related gene, c-hairy2 , which also...

... Notch hindbrain presomitic mesoderm neurogenesis mouse Drosophila Intercellular signaling is a fundamental feature underlying the establishment of pattern during embryonic development ( Jessell and Melton, 1992 ). Theoretical models predict that an important aspect of embryonic...

...Patrick P. L. Tam Orthotopic grafts of wheat germ agglutinin-colloidal gold conjugate (WGA-gold) labelled cells were used to demonstrate differences in the segmental fate of cells in the presomitic mesoderm of the early-somite-stage mouse embryos developing in vitro . Labelled cells in the anterior...

...P. P. L. Tam ABSTRACT Consistently six to seven somites were formed in explants of presomitic mesoderm of 8·5-day to 11·5-day mouse embryos and this number correlated well with the number of somitomeres previously identified in the same tissue by stereo SEM (Tam, Meier & Jacobson, 1982...