... overlapping but distinct expression profiles, with high NANOGP1 expression restricted to early epiblast cells and naïve-state pluripotent stem cells. Sequence analysis and epitope-tagging revealed that NANOGP1 is protein coding with an intact homeobox domain. The duplication that created NANOGP1 occurred...

...Anish Dattani; Tao Huang; Corin Liddle; Austin Smith; Ge Guo ABSTRACT Propagation of human naïve pluripotent stem cells (nPSCs) relies on the inhibition of MEK/ERK signalling. However, MEK/ERK inhibition also promotes differentiation into trophectoderm (TE). Therefore, robust self-renewal requires...

... oxygen tension) induces WNT pathway activity and is harnessed to enhance differentiation of embryonic stem cells into gastruloids. Hypoxia Hif1a WNT Gastruloid Pluripotency Max-Planck-Gesellschaft Bundesinstitut für Risikobewertung http://dx.doi.org/10.13039/501100008751...

... and MYC (OSKM) enables reprogramming of differentiated cells into pluripotent embryonic stem cells. Methods based on partial and reversible in vivo reprogramming are a promising strategy for tissue regeneration and rejuvenation. However, little is known about the barriers that impair reprogramming...

... ABSTRACT Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the embryo uncertain. Here, we avoided using feeder...

... and function, microtubules have received less attention when unravelling the regulatory circuitry of pluripotency. Here, we summarise the role of microtubule organisation and microtubule-dependent events required for the formation of pluripotent cells in vivo by deciphering the process of early embryogenesis...

...Nicola Festuccia; Nick Owens; Almira Chervova; Agnès Dubois; Pablo Navarro ABSTRACT The maintenance of pluripotency in mouse embryonic stem cells (ESCs) is governed by the action of an interconnected network of transcription factors. Among them, only Oct4 and Sox2 have been shown to be strictly...

... et al., 2011 , 2012 ) ( Fig. 2 A). Importantly, EpiLCs are considered equivalent to post-EPI cells in the E5.5 embryo, and are thought to represent a ‘formative state ’ of pluripotency ( Smith, 2017 ). In contrast, mouse ESCs cultured in 2i+LIF are equivalent to pre-EPI cells of the blastocyst...

..., they are referred to as pluripotent, rather than totipotent. Here, we consider the experimental potential of various ESC populations and a number of recently identified in vitro culture systems producing states beyond pluripotency and reminiscent of those observed during pre-implantation development. We also...

...Zhenhua Zhu; Yan Zhang; Xiaoxiao Wang; Xiaohu Wang; Shou-Dong Ye ABSTRACT The identification of novel mechanisms to maintain embryonic stem cell (ESC) pluripotency is of crucial importance, because the currently used culture conditions are not suitable for ESCs from all species. In this study, we...

...Sofiane Hamidi; Yukiko Nakaya; Hiroki Nagai; Cantas Alev; Takeya Kasukawa; Sapna Chhabra; Ruda Lee; Hitoshi Niwa; Aryeh Warmflash; Tatsuo Shibata; Guojun Sheng ABSTRACT The pluripotent epiblast gives rise to all tissues and organs in the adult body. Its differentiation starts at gastrulation, when...

... development and in embryonic stem cells by maintaining pluripotency or by regulating differentiation. In this Primer, we provide an updated overview of TET functions in development and stem cells. We discuss the catalytic and non-catalytic activities of TETs, and their roles as epigenetic regulators of both...

.... , McManus , M. T. , Plath , K. , Meshorer , E. , et al. ( 2009 ). Chd1 regulates open chromatin and pluripotency of embryonic stem cells . Nature   460 , 863 - 868 . 10.1038/nature08212 Gaspar-Maia , A. , Alajem , A. , Meshorer , E. and Ramalho-Santos , M. ( 2011...

...Deniz Gökbuget; Robert Blelloch ABSTRACT Pluripotent stem cells give rise to all cells of the adult organism, making them an invaluable tool in regenerative medicine. In response to differentiation cues, they can activate markedly distinct lineage-specific gene networks while turning off...

...Alexander M. Tsankov; Marc H. Wadsworth, II; Veronika Akopian; Jocelyn Charlton; Samuel J. Allon; Aleksandra Arczewska; Benjamin E. Mead; Riley S. Drake; Zachary D. Smith; Tarjei S. Mikkelsen; Alex K. Shalek; Alexander Meissner ABSTRACT Maintenance of pluripotency and specification towards a new...

... during their transition to primitive endoderm. He reported that, despite the transcriptional repression of certain pluripotency genes during differentiation, the TFs that regulate these genes remain stable at the protein level and can remain bound to their corresponding enhancers, thus rapidly...

...://www.biologists.com/user-licence-1-1/ Summary: A novel nuclear inhibitor holds Tcf7l1 in a transcriptional inhibitory state and promotes silencing of Wnt target genes. Organizer Groucho/Tle Tcf/Lef Barhl2 Hdac Stem cells Wnt Pluripotency Transcription References Abramoff , M. D...

..., gene regulation and network control of naive pluripotency, and the transition path from pluripotency to lineage commitment ( Blaschke et al., 2013 ; Carey et al., 2014 ; Dunn et al., 2014 ; Ficz et al., 2013 ; Habibi et al., 2013 ; Hackett et al., 2017 ; Hayashi et al., 2012 , 2011 ; Kalkan et...

... expression signatures are species, stage and lineage specific. The pluripotency network in the primate epiblast lacks certain regulators that are operative in mouse, but encompasses WNT components and genes associated with trophoblast specification. Sequential activation of GATA6, SOX17 and GATA4 markers...

... for HDAC activity initiates in naïve blastula cells; HDAC inhibition causes loss of pluripotency gene expression and blocks the ability of blastula stem cells to contribute to lineages of the three embryonic germ layers. We find that pluripotent naïve blastula cells and neural crest cells are both...