... cells. A critical event regulated by EGFR signaling is the repression of the sulfotransferase-encoding gene pipe in dorsal follicle cells, but how this occurs remains unclear. Here we show that Mirror (Mirr), a homeodomain transcription factor induced by EGFR signaling in DA follicle cells, directly...

... is determined by the expression of the pipe gene in a subpopulation of ventral follicle cells in the developing egg chamber. The Pipe protein exhibits homology to vertebrate glycosaminoglycan sulfotransferases. In a previous study, we demonstrated that embryonic DV polarity depends upon the sulfotransferase...

...Xianjun Zhu; Jonaki Sen; Leslie Stevens; Jason S. Goltz; David Stein The Drosophila pipe gene encodes ten related proteins that exhibit amino acid sequence similarity to vertebrate heparan sulfate 2-O-sulfotransferase. One of the Pipe isoforms, which is expressed in the ventral follicular...

...Francesca Peri; Martin Technau; Siegfried Roth The restriction of Pipe, a potential glycosaminoglycan-modifying enzyme, to ventral follicle cells of the egg chamber is essential for dorsoventral axis formation in the Drosophila embryo. pipe repression depends on the TGFα-like ligand Gurken, which...

... patterns the somatic follicular epithelium, establishing the dorsoventral asymmetry of eggshell and embryo. Analysis of follicle cell clones homozygous for a null allele of Ras demonstrates that Ras is required cell-autonomously to repress pipe transcription, the critical first step in embryonic...

... ligand, Gurken. As a consequence, pipe is transcribed in a broad ventral domain to initiate the Toll signaling pathway in the embryo, resulting in a gradient of Dorsal nuclear translocation. We show that expression of pipe RNA requires the action of fettucine ( fet ) in ovarian follicle cells. Loss...

... duplication gurken pipe spätzle An enduring problem in developmental biology is understanding how spatial asymmetry is initiated and gradually modified to produce pattern across a body axis. In amphibians, early development involves establishment of the organizer, which was defined by its...

...Jonaki Sen; Jason S. Goltz; Mary Konsolaki; Trudi Schüpbach; David Stein ABSTRACT Drosophila embryonic dorsal-ventral polarity originates in the ovarian follicle through the restriction of pipe gene expression to a ventral subpopulation of follicle cells. Pipe, a homolog of vertebrate...

... . 10.1093/genetics/139.3.1347 Sen , J. , Goltz , J. S. , Stevens , L. and Stein , D. ( 1998 ). Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal- ventral polarity . Cell 95 , 471 – 481 . 10.1016/S0092-8674(00)81615-3...