... is known about pig pancreas development. To fill this knowledge gap, we investigated fetal and neonatal pig pancreas at multiple, crucial developmental stages using modern experimental approaches. Purification of islet β-, α- and δ-cells followed by transcriptome analysis (RNA-seq) and immunohistology...

...Tristan J. Vaughan; Peter S. James; John C. Pascall; Kenneth D. Brown ABSTRACT Expression of mRNA for transforming growth factoralpha (TGF-α), epidermal growth factor (EGF) and the epidermal growth factor receptor (EGF-R) during early pig development was evaluated by reverse transcription-PCR...

... in frequency and amplitude. Overall, the results demonstrate that, although electrostimulation induces both cortical granule exocytosis and protein reprogramming in porcine eggs, it does not reproduce the pattern of [Ca 2+ ] i changes induced by sperm entry at fertilization. Pig ovaries were obtained from...

...A. Whyte; H. J. Stewart ABSTRACT Blastocyst material was obtained from 25 pigs during the period 10 to 33 days post coitum , and fixed thin sections of tissue were hybridized in situ to sense and antisense fos RNA probes synthesized using the expression vector Bluescribe M13 + . Indirect...

...V. E. Papaioannou; K. M. Ebert Total cell number as well as differential cell numbers representing the inner cell mass (ICM) and trophectoderm were determined by a differential staining technique for preimplantation pig embryos recovered between 5 and 8 days after the onset of oestrus. Total cell...

...C. J. A. H. V. Van Vorstenbosch; E. Spek; B. Colenbrander; C. J. G. Wensing The development of germ cells in the male pig was investigated ultrastructurally in normal and decapitated fetuses. The age ranged respectively from 30 days p . c . till one month after birth and from 52 days p . c . until...